Optimal Cutting Temperature Compound
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Optimal Cutting Temperature Compound
Optimal cutting temperature compound (OCT compound) is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a sample onto slides for analysis. Components As stated on the bottle: * 10.24% polyvinyl alcohol * 4.26% polyethylene glycol * 85.5% non-reactive ingredients" See also * Microtome *Cryostat A cryostat (from ''cryo'' meaning cold and ''stat'' meaning stable) is a device used to maintain low cryogenic temperatures of samples or devices mounted within the cryostat. Low temperatures may be maintained within a cryostat by using various r ... References Further reading * Histology {{pathology-stub ...
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Tissue For Frozen Section In Cryostat
Tissue may refer to: Biology * Tissue (biology), an ensemble of similar (or dissimilar in structure but same in origin) cells that together carry out a specific function * ''Triphosa haesitata'', a species of geometer moth ("tissue moth") found in North America * ''Triphosa dubitata'', a species of geometer moth ("tissue") found in Afro-Eurasia Paper products * Tissue paper, a type of thin, gauzy translucent paper used for wrapping and cushioning items ** Facial tissue, tissue paper used for cleaning the face ** Japanese tissue, tissue paper from Japan made of vegetable fibers ** Toilet paper, tissue paper used for cleaning the anus ** Wrapping tissue, tissue paper used for wrapping and cushioning items Other * Aerial tissue Aerial silks (also known as aerial contortion, aerial ribbons, aerial tissues, fabric, ribbon, or ''tissu'') is a type of performance in which one or more artists perform aerial acrobatics while hanging from a specialist fabric. The fabric may ..., an ac ...
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Frozen Section Procedure
The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery. The technical name for this procedure is cryosection. The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome. The quality of the slides produced by frozen section is of lower quality than formalin fixed paraffin embedded tissue processing. While diagnosis can be rendered in many cases, fixed tissue processing is preferred in many conditions for more accurate diagnosis. The intraoperative consultation is the name given to the whole intervention by the pathologist, which includes not only frozen section but also gross evaluation of the specimen, examination of cytology preparations taken on the specimen (e.g. touch imprints), and aliquoting of the specimen for special studies (e.g. molecular pathology techniques, flow cytometry). The report given by the pathologist is often limited to ...
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Microtome
A microtome (from the Greek ''mikros'', meaning "small", and ''temnein'', meaning "to cut") is a cutting tool used to produce extremely thin slices of material known as ''sections''. Important in science, microtomes are used in microscopy, allowing for the preparation of samples for observation under transmitted light or electron radiation. Microtomes use steel, glass or diamond blades depending upon the specimen being sliced and the desired thickness of the sections being cut. Steel blades are used to prepare histological sections of animal or plant tissues for light microscopy. Glass knives are used to slice sections for light microscopy and to slice very thin sections for electron microscopy. Industrial grade diamond knives are used to slice hard materials such as bone, teeth and tough plant matter for both light microscopy and for electron microscopy. Gem-quality diamond knives are also used for slicing thin sections for electron microscopy. Microtomy is a method for the p ...
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Cryostat
A cryostat (from ''cryo'' meaning cold and ''stat'' meaning stable) is a device used to maintain low cryogenic temperatures of samples or devices mounted within the cryostat. Low temperatures may be maintained within a cryostat by using various refrigeration methods, most commonly using cryogenic fluid bath such as liquid helium. Hence it is usually assembled into a vessel, similar in construction to a vacuum flask or Dewar. Cryostats have numerous applications within science, engineering, and medicine. Types Closed-cycle cryostats Closed-cycle cryostats consist of a chamber through which cold helium vapour is pumped. An external mechanical refrigerator extracts the warmer helium exhaust vapour, which is cooled and recycled. Closed-cycle cryostats consume a relatively large amount of electrical power, but need not be refilled with helium and can run continuously for an indefinite period. Objects may be cooled by attaching them to a metallic coldplate inside a vacuum ch ...
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Microtome
A microtome (from the Greek ''mikros'', meaning "small", and ''temnein'', meaning "to cut") is a cutting tool used to produce extremely thin slices of material known as ''sections''. Important in science, microtomes are used in microscopy, allowing for the preparation of samples for observation under transmitted light or electron radiation. Microtomes use steel, glass or diamond blades depending upon the specimen being sliced and the desired thickness of the sections being cut. Steel blades are used to prepare histological sections of animal or plant tissues for light microscopy. Glass knives are used to slice sections for light microscopy and to slice very thin sections for electron microscopy. Industrial grade diamond knives are used to slice hard materials such as bone, teeth and tough plant matter for both light microscopy and for electron microscopy. Gem-quality diamond knives are also used for slicing thin sections for electron microscopy. Microtomy is a method for the p ...
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Cryostat
A cryostat (from ''cryo'' meaning cold and ''stat'' meaning stable) is a device used to maintain low cryogenic temperatures of samples or devices mounted within the cryostat. Low temperatures may be maintained within a cryostat by using various refrigeration methods, most commonly using cryogenic fluid bath such as liquid helium. Hence it is usually assembled into a vessel, similar in construction to a vacuum flask or Dewar. Cryostats have numerous applications within science, engineering, and medicine. Types Closed-cycle cryostats Closed-cycle cryostats consist of a chamber through which cold helium vapour is pumped. An external mechanical refrigerator extracts the warmer helium exhaust vapour, which is cooled and recycled. Closed-cycle cryostats consume a relatively large amount of electrical power, but need not be refilled with helium and can run continuously for an indefinite period. Objects may be cooled by attaching them to a metallic coldplate inside a vacuum ch ...
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