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Maxicell
Maxicell is a system for identifying plasmid-encoded proteins that was developed by Aziz Sancar, Sancar, AM Hack, and WD Rupp.Sancar A, Hack AM, Rupp WD. J Bacteriol. 1979 Simple method for identification of plasmid-coded proteins. 137(1):692-3. The method uses a mutant strain of the bacterium ''Escherichia coli'' that is defective in repairing DNA damage. Irradiating the mutant cells with UV light leads to degradation of the bacterial chromosome. Plasmids in the cells mostly escape the UV-induced damage because of their small size. Proteins encoded by genes on the bacterial chromosome will no longer be synthesized, while proteins encoded by genes on the plasmid DNA molecules will continue to be made. The addition of radioactively-labeled amino acids following the UV treatment allows the plasmid-encoded proteins to be specifically visualized, because proteins synthesized prior to the UV treatment will not contain the radioactive label. The method was used for amplification of photo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Aziz Sancar
Aziz Sancar (born 8September 1946) is a Turkish molecular biologist specializing in DNA repair, cell cycle checkpoints, and circadian clock. In 2015, he was awarded the Nobel Prize in Chemistry along with Tomas Lindahl and Paul L. Modrich for their mechanistic studies of DNA repair. He has made contributions on photolyase and nucleotide excision repair in bacteria that have changed his field. Sancar is currently the Sarah Graham Kenan Professor of Biochemistry and Biophysics at the University of North Carolina School of Medicine and a member of the UNC Lineberger Comprehensive Cancer Center. He is the co-founder of the Aziz & Gwen Sancar Foundation, which is a non-profit organization to promote Turkish culture and to support Turkish students in the United States. Early life Aziz Sancar was born on 8 September 1946 to a lower-middle-class family in the Savur district of Mardin Province, southeastern Turkey. His oldest brother Kenan Sancar is a retired brigadier general in th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
