Interferometric Microscopy
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Interferometric Microscopy
Interferometric microscopy or imaging interferometric microscopy is the concept of microscopy which is related to holography, synthetic-aperture imaging, and off-axis-dark-field illumination techniques. Interferometric microscopy allows enhancement of resolution of optical microscopy due to interferometric (holographic) registration of several partial images (amplitude and phase) and the numerical combining. Combining of partial images In interferometric microscopy, the image of a micro-object is synthesized numerically as a coherent combination of partial images with registered amplitude and phase. For registration of partial images, a conventional holographic set-up is used with a reference wave, as is usual in optical holography. Capturing multiple exposures allows the numerical emulation of a large numerical aperture objective from images obtained with an objective lens with smaller-value numerical aperture. Similar techniques allows scanning and precise detection of small par ...
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Holography
Holography is a technique that enables a wavefront to be recorded and later re-constructed. Holography is best known as a method of generating real three-dimensional images, but it also has a wide range of other applications. In principle, it is possible to make a hologram for any type of wave. A hologram is made by superimposing a second wavefront (normally called the reference beam) on the wavefront of interest, thereby generating an interference pattern which is recorded on a physical medium. When only the second wavefront illuminates the interference pattern, it is diffracted to recreate the original wavefront. Holograms can also be computer-generated by modelling the two wavefronts and adding them together digitally. The resulting digital image is then printed onto a suitable mask or film and illuminated by a suitable source to reconstruct the wavefront of interest. Overview and history The Hungarian- British physicist Dennis Gabor (in Hungarian: ''Gábor Déne ...
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Journal Of Physics B
The ''Journal of Physics B: Atomic, Molecular and Optical Physics'' is a biweekly peer-reviewed scientific journal published by IOP Publishing. It was established in 1968 from the division of the earlier title, ''Proceedings of the Physical Society''. In 2006, the ''Journal of Optics B, Journal of Optics B: Quantum and Semiclassical Optics'' was merged with the ''Journal of Physics B''. The editor-in-chief is Marc Vrakking (Max Born Institute for Nonlinear Optics and Short Pulse Spectroscopy). Scope The journal covers research on atomic physics, atomic, molecular physics, molecular, and optical physics. Topics include atomic and molecular structure, spectra and collisions, ultracold matter, quantum optics and non linear optics, quantum information, laser physics, intense laser fields, ultrafast and x-ray physics and atomic and molecular physics in plasmas. The journal publishes research papers, fast track communications, topical reviews, tutorials, and invited articles. It occasi ...
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Interferometry
Interferometry is a technique which uses the '' interference'' of superimposed waves to extract information. Interferometry typically uses electromagnetic waves and is an important investigative technique in the fields of astronomy, fiber optics, engineering metrology, optical metrology, oceanography, seismology, spectroscopy (and its applications to chemistry), quantum mechanics, nuclear and particle physics, plasma physics, remote sensing, biomolecular interactions, surface profiling, microfluidics, mechanical stress/strain measurement, velocimetry, optometry, and making holograms. Interferometers are devices that extract information from interference. They are widely used in science and industry for the measurement of microscopic displacements, refractive index changes and surface irregularities. In the case with most interferometers, light from a single source is split into two beams that travel in different optical paths, which are then combined again to produce ...
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Microscopy
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the o ...
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Diffraction Limited
The resolution of an optical imaging system a microscope, telescope, or camera can be limited by factors such as imperfections in the lenses or misalignment. However, there is a principal limit to the resolution of any optical system, due to the physics of diffraction. An optical system with resolution performance at the instrument's theoretical limit is said to be diffraction-limited. The diffraction-limited angular resolution of a telescopic instrument is inversely proportional to the wavelength of the light being observed, and proportional to the diameter of its objective's entrance aperture. For telescopes with circular apertures, the size of the smallest feature in an image that is diffraction limited is the size of the Airy disk. As one decreases the size of the aperture of a telescopic lens, diffraction proportionately increases. At small apertures, such as f/22, most modern lenses are limited only by diffraction and not by aberrations or other imperfections in the con ...
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Raman Microscope
The Raman microscope is a laser-based microscopic device used to perform Raman spectroscopy.''Microscopical techniques in the use of the molecular optics laser examiner Raman microprobe'', by M. E. Andersen, R. Z. Muggli, Analytical Chemistry, 1981, 53 (12), pp 1772–177/ref> The term MOLE (molecular optics laser examiner) is used to refer to the Raman-based microprobe. The technique used is named after C. V. Raman, who discovered the scattering properties in liquids. Configuration The Raman microscope begins with a standard optical microscope, and adds an excitation laser, laser rejection filters, a spectrometer or monochromator, and an optical sensitive detector such as a charge-coupled device (CCD), or photomultiplier tube, (PMT). Traditionally Raman microscopy was used to measure the Raman spectrum of a point on a sample, more recently the technique has been extended to implement Raman spectroscopy for direct chemical imaging over the whole field of view on a 3D sample. ...
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Numerical Aperture
In optics, the numerical aperture (NA) of an optical system is a dimensionless number that characterizes the range of angles over which the system can accept or emit light. By incorporating index of refraction in its definition, NA has the property that it is constant for a beam as it goes from one material to another, provided there is no refractive power at the interface. The exact definition of the term varies slightly between different areas of optics. Numerical aperture is commonly used in microscopy to describe the acceptance cone of an objective (and hence its light-gathering ability and resolution), and in fiber optics, in which it describes the range of angles within which light that is incident on the fiber will be transmitted along it. General optics In most areas of optics, and especially in microscopy, the numerical aperture of an optical system such as an objective lens is defined by :\mathrm = n \sin \theta, where is the index of refraction of the medium in ...
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Holography
Holography is a technique that enables a wavefront to be recorded and later re-constructed. Holography is best known as a method of generating real three-dimensional images, but it also has a wide range of other applications. In principle, it is possible to make a hologram for any type of wave. A hologram is made by superimposing a second wavefront (normally called the reference beam) on the wavefront of interest, thereby generating an interference pattern which is recorded on a physical medium. When only the second wavefront illuminates the interference pattern, it is diffracted to recreate the original wavefront. Holograms can also be computer-generated by modelling the two wavefronts and adding them together digitally. The resulting digital image is then printed onto a suitable mask or film and illuminated by a suitable source to reconstruct the wavefront of interest. Overview and history The Hungarian- British physicist Dennis Gabor (in Hungarian: ''Gábor Déne ...
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Digital Holographic Microscopy
Digital holographic microscopy (DHM) is digital holography applied to microscopy. Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image of the object. Instead, the light wave front information originating from the object is digitally recorded as a hologram, from which a computer calculates the object image by using a numerical reconstruction algorithm. The image forming lens in traditional microscopy is thus replaced by a computer algorithm. Other closely related microscopy methods to digital holographic microscopy are interferometric microscopy, optical coherence tomography and diffraction phase microscopy. Common to all methods is the use of a reference wave front to obtain amplitude (intensity) ''and'' phase information. The information is recorded on a digital image sensor or by a photodetector from which an image of the object is created (reconstructed) by a computer. In traditional microscopy, which do not u ...
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Atomic De Broglie Microscope
The atomic de Broglie microscope (also atomic nanoscope, neutral beam microscope, or scanning helium microscope when helium is used as the probing atom) is an imaging system which is expected to provide resolution at the nanometer scale. It is sometimes referred to as a "nanoscope." History The resolution of optical microscopes is limited to a few hundred nanometers by the wave properties of the light. The idea of imaging with atoms instead of light is widely discussed in the literature since the past century. Atom optics using neutral atoms instead of light could provide resolution as good as the electron microscope and be completely non-destructive, because short wavelengths on the order of a nanometer can be realized at low energy of the probing particles. "It follows that a helium microscope with nanometer resolution is possible. A helium atom microscope will be unique non-destructive tool for reflection or transmission microscopy." Focusing of neutral atoms Currently, t ...
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Synthetic Aperture
Aperture synthesis or synthesis imaging is a type of interferometry that mixes signals from a collection of telescopes to produce images having the same angular resolution as an instrument the size of the entire collection. At each separation and orientation, the lobe-pattern of the interferometer produces an output which is one component of the Fourier transform of the spatial distribution of the brightness of the observed object. The image (or "map") of the source is produced from these measurements. Astronomical interferometers are commonly used for high-resolution optical, infrared, submillimetre and radio astronomy observations. For example, the Event Horizon Telescope project derived the first image of a black hole using aperture synthesis. Technical issues Aperture synthesis is possible only if both the amplitude and the phase of the incoming signal are measured by each telescope. For radio frequencies, this is possible by electronics, while for optical frequencies, the ...
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Energetic Neutral Atom
Energetic Neutral Atom (ENA) imaging, often described as "seeing with atoms", is a technology used to create global images of otherwise invisible phenomena in the magnetospheres of planets and throughout the heliosphere. The solar wind is composed of plasma (physics), plasma that is emitted from the Sun. The solar wind plasma is mostly composed of hydrogen, bare electrons and protons, along with some other kinds of Atomic nucleus, nuclei, mostly helium. The interstellar medium, space between solar systems is similar, but they come from other stars in our galaxy. These Charged particle, charged particles can be redirected by magnetic fields; for instance, Earth's magnetic field deflects these solar wind particles around the Earth. Occasionally, charged particles within plasmas steal electrons from neutral atoms they run into, making them neutral and not subject to large-scale Electromagnetic field, electromagnetic fields and waves. Still moving very fast, they tend to travel mostl ...
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