Inoculation Needle
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Inoculation Needle
An inoculation needle is a laboratory equipment used in the field of microbiology to transfer and inoculate living microorganisms. It is one of the most commonly implicated biological laboratory tools and can be disposable or re-usable. A standard reusable inoculation needle is made from nichrome or platinum wire affixed to a metallic handle. A disposable inoculation needle is often made from plastic resin. The base of the needle is dulled, resulting in a blunted end. Uses Inoculation needles are primarily applied in microbiology for studying bacteria and fungi on semi-solid media. Biotechnology, cell biology and immunology may also utilize needle-oriented culture methods. The application of inoculation needles focuses on the inoculation and isolation of very defined regions of the cultures and the requirements of least disturbance between two closely crowded microbial colonies. It can also be used in harpooning under a low magnification microscope. Streaking on streak plates, ...
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Tip Of Inoculation Needle
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Microbiological Culture
A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as a research tool in molecular biology. The term ''culture'' can also refer to the microorganisms being grown. Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both. It is one of the primary diagnostic methods of microbiology and used as a tool to determine the cause of infectious disease by letting the agent multiply in a predetermined medium. For example, a throat culture is taken by scraping the lining of tissue in the back of the throat and blotting the sample into a medium to be able to screen for harmful microorganisms, such as '' Streptococcus pyogenes'', the causative agent of strep throat. Furthermore, the term culture is more generally used inform ...
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Inoculation Loop
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate.Ronald Westphal (1988): Microbiological Techniques in School', page 34. Document No. 28 in the series ''Science and Technology Education''. This process is called inoculation. The tool consists of a thin handle with a loop about 5 mm wide or smaller at the end. It was originally made of twisted metal wire (such as platinum, tungsten or nichrome), but disposable molded plastic versions are now common. See also * Cell spreader * Micropipette A pipette (sometimes spelled as pipett) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with diff ... References Labo ...
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Streaking (microbiology)
In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested. The modern streak plate method has progressed from the efforts of Robert Koch and other microbiologists to obtain microbiological cultures of bacteria in order to study them. The dilution or isolation by streaking method was first developed by Loeffler and Gaffky in Koch's laboratory, which involves the dilution of bacteria by systematically streaking them over the exterior of the agar in a Petri dish to obtain isolated colonies which will then grow into quantity of cells, or isolated colonies. If the agar surface grows microorganisms which are all genetically same, the culture is then considered as a microbiological culture. Technique Streaking is rapid and ideally a simple process ...
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Inoculation
Inoculation is the act of implanting a pathogen or other microorganism. It may refer to methods of artificially inducing immunity against various infectious diseases, or it may be used to describe the spreading of disease, as in "self-inoculation," the spreading of disease from one part of the body to another, or even to the spreading of bacteria in a Petri dish for culturing purposes. The terms "inoculation", "vaccination", and "immunization" are often used synonymously, but there are some important differences among them. Inoculation is the act of implanting a disease inside a person or animal, vaccination is the act of implanting or giving someone a vaccine specifically, and immunization is what happens to the immune system as a result. Terminology Until the early 1800s inoculation referred only to variolation (from the Latin word ''variola'' = smallpox), the predecessor to the smallpox vaccine. The smallpox vaccine, introduced by Edward Jenner in 1796, was called cowpox ...
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Stab Cultures
STAB or stab may refer to: *Stabbing, penetration or contact with a sharp object * Stab, Kentucky, US * Stab (b-boy move), a breakdance technique * Stab (music), an element in musical composition * Stab (Luftwaffe designation), during World War II, a German designation for command aircraft or headquarters units *Johann Stab, Johannes Stabius (1450–1522), Austrian cartographer *''Stab'', the film-within-a-film from the ''Scream'' franchise *"Stab", a song by Built to Spill from ''There's Nothing Wrong with Love'' Acronyms and abbreviations * Sodium triacetoxyborohydride, a reducing agent used in organic synthesis *St. Anne's-Belfield School, a college preparatory school in Charlottesville, Virginia, US *Same-type attack bonus, a scoring element in the gameplay of ''Pokémon'' video games * Symbol table, a data structure used by a language translator * Stab jacket or buoyancy compensator, a piece of diving equipment * Stabilizer (aircraft) *Strike Assault Boat See also * stabs ...
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Agar Plate
An agar plate is a Petri dish that contains a growth medium solidified with agar, used to culture microorganisms. Sometimes selective compounds are added to influence growth, such as antibiotics. Individual microorganisms placed on the plate will grow into individual colonies, each a clone genetically identical to the individual ancestor organism (except for the low, unavoidable rate of mutation). Thus, the plate can be used either to estimate the concentration of organisms in a liquid culture or a suitable dilution of that culture using a colony counter, or to generate genetically pure cultures from a mixed culture of genetically different organisms. Several methods are available to plate out cells. One technique is known as " streaking". In this technique, a drop of the culture on the end of a thin, sterile loop of wire, sometimes known as an inoculator, is streaked across the surface of the agar leaving organisms behind, a higher number at the beginning of the streak and a lo ...
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Vortex Mixer
A vortex mixer, or vortexer, is a simple device used commonly in laboratories to mix small vials of liquid. It consists of an electric motor with the drive shaft oriented vertically and attached to a cupped rubber piece mounted slightly off-center. As the motor runs the rubber piece oscillates rapidly in a circular motion. When a test tube or other appropriate container is pressed into the rubber cup (or touched to its edge) the motion is transmitted to the liquid inside and a vortex is created. Most vortex mixers are designed with 2 or 4-plate formats, have variable speed settings ranging from 100 to 3,200 rpm, and can be set to run continuously, or to run only when downward pressure is applied to the rubber piece. Use Vortex mixers are quite commonplace in bioscience laboratories. In cell culture and microbiology laboratories they may be used to suspend cells. In a biochemical or analytical laboratory they may be used to mix the reagents of an assay or to mix an experimen ...
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Inoculum
In biology, inoculum refers to the source material used for inoculation. ''Inoculum'' may refer to: * In medicine, material that is the source of the inoculation in a vaccine * In microbiology, propagules: cells, tissue, or viruses that are used to inoculate a new culture * Microbial inoculant, the beneficial introduction of microbes to improve plant health * A method of propagation of fungal plant disease transmission * Fermentation starter, in food production See also: * ''Fear Inoculum'', a 2019 album by American rock band Tool A tool is an object that can extend an individual's ability to modify features of the surrounding environment or help them accomplish a particular task. Although many animals use simple tools, only human beings, whose use of stone tools dates b ...
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Propagule
In biology, a propagule is any material that functions in propagating an organism to the next stage in its life cycle, such as by dispersal. The propagule is usually distinct in form from the parent organism. Propagules are produced by organisms such as plants (in the form of seeds or spores), fungi (in the form of spores), and bacteria (for example endospores or microbial cysts). In disease biology, pathogens are said to generate infectious propagules, the units that transmit a disease. These can refer to bacteria, viruses, fungi, or protists, and can be contained within host material. For instance, for influenza, the infectious propagules are carried in droplets of host saliva or mucus that are expelled during coughing or sneezing. In horticulture, a propagule is any plant material used for the purpose of plant propagation. In asexual reproduction, a propagule is often a stem cutting. In some plants, a leaf section or a portion of root can be used. In sexual reproduction, a ...
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Alcohol Burner
An alcohol burner or spirit lamp is a piece of laboratory equipment used to produce an open flame. It can be made from brass, glass, stainless steel or aluminium. Uses Alcohol burners are preferred for some uses over Bunsen burners for safety purposes, and in laboratories where natural gas is not available. Their flame is limited to approximately 5 centimeters (two inches) in height, with a comparatively lower temperature than the gas flame of the Bunsen burner. While they do not produce flames as hot as other types of burners, they are sufficiently hot for performing some chemistries, standard microbiology laboratory procedures, and can be used for flame sterilization of other laboratory equipment. Operation Typical fuel is denatured alcohol, methanol, or isopropanol. A cap is used as a snuffer for extinguishing the flame. See also * Bunsen burner * Heating mantle * Beverage-can stove * Portable stove A portable stove is a cooking stove specially designed to be ...
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