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Immune Electron Microscopy
Immune electron microscopy (more often called immunoelectron microscopy) is the equivalent of immunofluorescence, but it uses electron microscopy rather than light microscopy. Immunoelectron microscopy identifies and localizes a molecule of interest, specifically a protein of interest, by attaching it to a particular antibody. This bond can form before or after embedding the cells into slides. A reaction occurs between the antigen and antibody, causing this label to become visible under the microscope. Scanning electron microscopy is a viable option if the antigen is on the surface of the cell, but transmission electron microscopy may be needed to see the label if the antigen is within the cell. Process Antigens and their respective antibodies (usually two) interact in the section. Transmission electron microscopy then detects the antibody and, therefore, the protein. The second antibody is typically bound to gold because gold has a high atomic number, making it very dense. Col ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rotavirus With Gold- Labelled Monoclonal Antibody
''Rotavirus'' is a genus of double-stranded RNA viruses in the Family (taxonomy), family ''Reoviridae''. Rotaviruses are the most common cause of Diarrhea, diarrhoeal disease among infants and young children. Nearly every child in the world is infected with a rotavirus at least once by the age of five. Immunity (medical), Immunity develops with each infection, so subsequent infections are less severe. Adults are rarely affected. There are nine species of the genus, referred to as A, B, C, D, F, G, H, I and J. ''Rotavirus A'', the most common species, causes more than 90% of rotavirus infections in humans. The virus is transmitted by the fecal-oral route, faecal-oral route. It infects and damages the enterocyte, cells that line the small intestine and causes gastroenteritis (which is often called "stomach flu" despite having no relation to influenza). Although rotavirus was discovered in 1973 by Ruth Bishop and her colleagues by electron micrograph images and accounts for approx ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
