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Gapmer
Gapmers are short DNA antisense oligonucleotide structures with RNA-like segments on both sides of the sequence. These linear pieces of genetic information are designed to hybridize to a target piece of RNA and silence the gene through the induction of Ribonuclease H, RNase H cleavage. Binding of the gapmer to the target has a higher affinity due to the modified RNA flanking regions, as well as resistance to degradation by nucleases.QIAGEN. (2017). Antisense LNA GapmeRs Handbook: LNA-optimized oligonucleotides for strand-specific knockdown of mRNA and IncRNA. Germantown, MD: Author Gapmers are currently being developed as therapeutics for a variety of cancers, viruses, and other chronic genetic disorders. Chemical Structure Gapmers are composed of short DNA strands flanked by strands of RNA mimics. The mimics are typically composed of locked nucleic acids (LNA), 2′-O-methylation, 2'-OMe, or 2'-F modified bases. LNA sequences are RNA analogues "locked" into an ideal Base pair, Wa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gapmer Molecular Structure
Gapmers are short DNA antisense oligonucleotide structures with RNA-like segments on both sides of the sequence. These linear pieces of genetic information are designed to hybridize to a target piece of RNA and silence the gene through the induction of Ribonuclease H, RNase H cleavage. Binding of the gapmer to the target has a higher affinity due to the modified RNA flanking regions, as well as resistance to degradation by nucleases.QIAGEN. (2017). Antisense LNA GapmeRs Handbook: LNA-optimized oligonucleotides for strand-specific knockdown of mRNA and IncRNA. Germantown, MD: Author Gapmers are currently being developed as therapeutics for a variety of cancers, viruses, and other chronic genetic disorders. Chemical Structure Gapmers are composed of short DNA strands flanked by strands of RNA mimics. The mimics are typically composed of locked nucleic acids (LNA), 2′-O-methylation, 2'-OMe, or 2'-F modified bases. LNA sequences are RNA analogues "locked" into an ideal Base pair, Wa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
