Filter Fluorometer

	Filter Fluorometer A filter fluorometer is a type of fluorometer that may be employed in fluorescence spectroscopy. In the fluorometer, a light source emits light of an excitation wavelength that is relevant to the compound to be measured. Filter fluorometers produce specific excitation and emission wavelengths by using optical filters. The filter blocks other wavelengths but transmits wavelengths relevant to the compound. The light passes through the sample to be measured, and a certain wavelength is absorbed while a longer wavelength is emitted. The emitted light is measured by a detector. By changing the optical filter, different substances can be measured. Applications Filter fluorometers can be highly sensitive, so are well suited to precise scientific research. The optical filters are relatively inexpensive and are easy to change, so filter fluorometers are commonly used in experimental applications which repeatedly measure different compounds. Filters There are two filters for the fluorom ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]
picture info	Fluorometer A fluorometer, fluorimeter or fluormeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. These parameters are used to identify the presence and the amount of specific molecules in a medium. Modern fluorometers are capable of detecting fluorescent molecule concentrations as low as 1 part per trillion. Fluorescence analysis can be orders of magnitude more sensitive than other techniques. Applications include chemistry/biochemistry, medicine, environmental monitoring. For instance, they are used to measure chlorophyll fluorescence to investigate plant physiology. Components and Design Typically fluorometers utilize a double beam. These two beams work in tandem to decrease the noise created from radiant power fluctuations. The upper beam is passed through a filter or monochromator and passes through the sample. The lower beam is passed through an ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]
picture info	Fluorescence Spectroscopy Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light. A complementary technique is absorption spectroscopy. In the special case of single molecule fluorescence spectroscopy, intensity fluctuations from the emitted light are measured from either single fluorophores, or pairs of fluorophores. Devices that measure fluorescence are called fluorometers. Theory Molecules have various states referred to as energy levels. Fluorescence spectroscopy is primarily concerned with electronic and vibrational states. Generally, the species being examined has a ground electronic state (a low energy state) of interest, and an excited electronic state of higher energy. Within each of these elec ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]
picture info	Fluorescence Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, than the absorbed radiation. A perceptible example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the electromagnetic spectrum (invisible to the human eye), while the emitted light is in the visible region; this gives the fluorescent substance a distinct color that can only be seen when the substance has been exposed to UV light. Fluorescent materials cease to glow nearly immediately when the radiation source stops, unlike phosphorescent materials, which continue to emit light for some time after. Fluorescence has many practical applications, including mineralogy, gemology, medicine, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, vacu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]
	Incident Incident may refer to: * A property of a graph in graph theory * ''Incident'' (film), a 1948 film noir * Incident (festival), a cultural festival of The National Institute of Technology in Surathkal, Karnataka, India * Incident (Scientology), a concept in Scientology * Incident ray, a ray of light that strikes a surface See also * Accident * The Incident (other) * Incidence (other) * Incident management (ITSM), an IT service management process to identify and correct service operation failures * Incident management, the activities of an organization to identify, analyze and correct organizational hazards * Nuclear and radiation accidents and incidents A nuclear and radiation accident is defined by the International Atomic Energy Agency (IAEA) as "an event that has led to significant consequences to people, the environment or the facility. Examples include lethal effects to individuals, lar ... , an irregularity with a nuclear installation not classi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]
picture info	Fluorescent Light A fluorescent lamp, or fluorescent tube, is a low-pressure mercury-vapor gas-discharge lamp that uses fluorescence to produce visible light. An electric current in the gas excites mercury vapor, which produces short-wave ultraviolet light that then causes a phosphor coating on the inside of the lamp to glow. A fluorescent lamp converts electrical energy into useful light much more efficiently than an incandescent lamp. The typical luminous efficacy of fluorescent lighting systems is 50–100 lumens per watt, several times the efficacy of incandescent bulbs with comparable light output. For comparison, the luminous efficacy of an incandescent bulb may only be 16 lumens per watt. Fluorescent lamp fixtures are more costly than incandescent lamps because, among other things, they require a ballast to regulate current through the lamp, but the initial cost is offset by a much lower running cost. Compact fluorescent lamps are now available in the same popular sizes as incand ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]
picture info	Emission Spectrum The emission spectrum of a chemical element or chemical compound is the spectrum of frequencies of electromagnetic radiation emitted due to an electron making a atomic electron transition, transition from a high energy state to a lower energy state. The photon energy of the emitted photon is equal to the energy difference between the two states. There are many possible electron transitions for each atom, and each transition has a specific energy difference. This collection of different transitions, leading to different radiated wavelengths, make up an emission spectrum. Each element's emission spectrum is unique. Therefore, spectroscopy can be used to identify elements in matter of unknown composition. Similarly, the emission spectra of molecules can be used in chemical analysis of substances. Emission In physics, emission is the process by which a higher energy quantum mechanical state of a particle becomes converted to a lower one through the emission of a photon, resulting in ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]
picture info	Excitation Spectrum Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light. A complementary technique is absorption spectroscopy. In the special case of single molecule fluorescence spectroscopy, intensity fluctuations from the emitted light are measured from either single fluorophores, or pairs of fluorophores. Devices that measure fluorescence are called fluorometers. Theory Molecules have various states referred to as energy levels. Fluorescence spectroscopy is primarily concerned with electronic and vibrational states. Generally, the species being examined has a ground electronic state (a low energy state) of interest, and an excited electronic state of higher energy. Within each of these elect ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]