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Fast Protein Liquid Chromatography
Fast protein liquid chromatography (FPLC), is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different Affinity (chemistry), affinities for two materials, a moving fluid (the Elution, mobile phase) and a porous solid (the Stationary phase (chemistry), stationary phase). In FPLC the mobile phase is an aqueous solution, or "Buffer solution, buffer". The buffer flow rate is controlled by a positive-displacement pump and is normally kept constant, while the composition of the buffer can be varied by drawing fluids in different proportions from two or more external reservoirs. The stationary phase is a resin composed of beads, usually of cross-linked agarose, packed into a cylindrical glass or plastic column. FPLC resins are available in a wide range of bead sizes and surface ligands depending on the application. In the most comm ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chromatography
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the ''stationary phase'' is fixed. Because the different constituents of the mixture tend to have different affinities for the stationary phase and are retained for different lengths of time depending on their interactions with its surface sites, the constituents travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and the stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation. Chromatography may be preparative or analytical. The purpose of preparativ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ion Exchange
Ion exchange is a reversible interchange of one kind of ion present in an insoluble solid with another of like charge present in a solution surrounding the solid with the reaction being used especially for softening or making water demineralised, the purification of chemicals and separation of substances. Ion exchange usually describes a process of purification of aqueous solutions using solid polymeric ion-exchange resin. More precisely, the term encompasses a large variety of processes where ions are exchanged between two electrolytes. Aside from its use to purify drinking water, the technique is widely applied for purification and separation of a variety of industrially and medicinally important chemicals. Although the term usually refers to applications of synthetic (man-made) resins, it can include many other materials such as soil. Typical ion exchangers are ion-exchange resins (functionalized porous or gel polymer), zeolites, montmorillonite, clay, and soil humus. Ion exc ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Size Exclusion Chromatography
Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. The chromatography column is packed with fine, porous beads which are commonly composed of dextran, agarose, or polyacrylamide polymers. The pore sizes of these beads are used to estimate the dimensions of macromolecules. SEC is a widely used polymer characterization method because of its ability to provide good molar mass distribution (Mw) results for polymers. Applications The main application of gel-filtration chr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Flow Limiter
A flow limiter or flow restrictor is a device to restrict the flow of a fluid, in general a gas or a liquid. Some designs use single stage or multi stage orifice plates to handle high and low flow rates. Flow limiters are often used in manufacturing plants as well as households. Safety is usually the main purpose of using a flow limiter. An example is manufacturing facilities and laboratories using flow limiters to prevent injury or death from noxious gases that are in use. The flow limiter prevents gases from causing injury or death by reducing its cross-sectional area where gas flows. Uses * Reduce flow of fluid (velocity) through a system, e.g. to reduce water usage in a shower * Reduce the amount of gas passing through a system * Reduce pressure in a system * Applications in medical instrumentation * As a safety valve to provide limited flow after closing in the event of a broken hose. (See '' Hydraulic fuse''). Specifications *Orifice diameter *Flow tolerance *Media temperat ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Degasser
A degasser is a device used in the upstream oil industry to remove dissolved and entrained gases from a liquid. In drilling it is used to remove gasses from drilling fluid which could otherwise form bubbles. In a produced water treatment plant it is part of the process to clean produced water prior to disposal. Degasser For a small amount of entrained gas in a drilling fluid, the degasser can play a major role of removing small bubbles that a liquid film has enveloped and entrapped. In order for it to be released and break out the air and gas such as methane, H2S and CO2 from the mud to the surface, the drilling fluid must pass through a degassing technique, and it can be accomplished by the equipment called a degasser, which is also a major part of mud systems. Another function of a degasser in the oil industry is to remove dissolved gases from a produced water stream as part of the water clean up process prior to its disposal. Types of Degasser Vacuum Tank Degasser Vacuum Type ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Peristaltic Pump
A peristaltic pump, also commonly known as a roller pump, is a type of positive displacement pump used for pumping a variety of fluids. The fluid is contained in a flexible tube fitted inside a circular pump casing. Most peristaltic pumps work through rotary motion, though linear peristaltic pumps have also been made. The rotor has a number of "wipers" or "rollers" attached to its external circumference, which compress the flexible tube as they rotate by. The part of the tube under compression is closed, forcing the fluid to move through the tube. Additionally, as the tube opens to its natural state after the rollers pass, more fluid is drawn into the tube. This process is called peristalsis and is used in many biological systems such as the gastrointestinal tract. Typically, there will be two or more rollers compressing the tube, trapping a body of fluid between them. The body of fluid is transported through the tube, toward the pump outlet. Peristaltic pumps may run continuous ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Piston Pump
A piston pump is a type of positive displacement pump where the high-pressure seal reciprocates with the piston. Piston pumps can be used to move liquids or compress gases. They can operate over a wide range of pressures. High pressure operation can be achieved without adversely affecting flow rate. Piston pumps can also deal with viscous media and media containing solid particles. This pump type functions through a piston cup, oscillation mechanism where down-strokes cause pressure differentials, filling of pump chambers, where up-stroke forces the pump fluid out for use. Piston pumps are often used in scenarios requiring high, consistent pressure and in water irrigation or delivery systems. Types The two main types of piston pump are the lift pump and the force pump. Both types may be operated either by hand or by an engine. Lift pump In a lift pump, the upstroke of the piston draws water, through a valve, into the lower part of the cylinder. On the downstroke, water passes thr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Protein Purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the specification of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Ideally, to study a protein of interest, it must be separated from other components of the cell so that contaminants won't interfere in the examination of the protein of interest's structure and function. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate. Purpose The protein manufacturing cost remains high and there is a g ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mass Spectrometry
Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio of ions. The results are presented as a ''mass spectrum'', a plot of intensity as a function of the mass-to-charge ratio. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures. A mass spectrum is a type of plot of the ion signal as a function of the mass-to-charge ratio. These spectra are used to determine the elemental or isotopic signature of a sample, the masses of particles and of molecules, and to elucidate the chemical identity or structure of molecules and other chemical compounds. In a typical MS procedure, a sample, which may be solid, liquid, or gaseous, is ionized, for example by bombarding it with a beam of electrons. This may cause some of the sample's molecules to break up into positively charged fragments or simply become positively charged without fragmenting. These ions (fragments) are then separated accordin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Matrix-assisted Laser Desorption/ionization
In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules ( biopolymers such as DNA, proteins, peptides and carbohydrates) and various organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is similar in character to electrospray ionization (ESI) in that both techniques are relatively soft (low fragmentation) ways of obtaining ions of large molecules in the gas phase, though MALDI typically produces far fewer multi-charged ions. MALDI methodology is a three-step process. First, the sample is mixed with a suitable matrix material and applied to a metal plate. Second, a pulsed laser irradiates the sample, triggering ablation and desorption of the sample and matrix materi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Volumetric Flow Rate
In physics and engineering, in particular fluid dynamics, the volumetric flow rate (also known as volume flow rate, or volume velocity) is the volume of fluid which passes per unit time; usually it is represented by the symbol (sometimes ). It contrasts with mass flow rate, which is the other main type of fluid flow rate. In most contexts a mention of ''rate of fluid flow'' is likely to refer to the volumetric rate. In hydrometry, the volumetric flow rate is known as '' discharge''. Volumetric flow rate should not be confused with volumetric flux, as defined by Darcy's law and represented by the symbol , with units of m3/(m2·s), that is, m·s−1. The integration of a flux over an area gives the volumetric flow rate. The SI unit is cubic metres per second (m3/s). Another unit used is standard cubic centimetres per minute (SCCM). In US customary units and imperial units, volumetric flow rate is often expressed as cubic feet per second (ft3/s) or gallons per minute (either ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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