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Doubled Haploidy
A doubled haploid (DH) is a genotype formed when haploid cells undergo chromosome doubling. Artificial production of doubled haploids is important in plant breeding. Haploid cells are produced from pollen or egg cells or from other cells of the gametophyte, then by induced or spontaneous chromosome doubling, a doubled haploid cell is produced, which can be grown into a doubled haploid plant. If the original plant was diploid, the haploid cells are monoploid, and the term doubled monoploid may be used for the doubled haploids. Haploid organisms derived from tetraploids or hexaploids are sometimes called dihaploids (and the doubled dihaploids are, respectively, tetraploid or hexaploid). Conventional inbreeding procedures take six generations to achieve approximately complete homozygosity, whereas doubled haploidy achieves it in one generation. Dihaploid plants derived from tetraploid crop plants may be important for breeding programs that involve diploid wild relatives of the crops ...
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Haploid
Ploidy () is the number of complete sets of chromosomes in a cell, and hence the number of possible alleles for autosomal and pseudoautosomal genes. Sets of chromosomes refer to the number of maternal and paternal chromosome copies, respectively, in each homologous chromosome pair, which chromosomes naturally exist as. Somatic cells, tissues, and individual organisms can be described according to the number of sets of chromosomes present (the "ploidy level"): monoploid (1 set), diploid (2 sets), triploid (3 sets), tetraploid (4 sets), pentaploid (5 sets), hexaploid (6 sets), heptaploid or septaploid (7 sets), etc. The generic term polyploid is often used to describe cells with three or more chromosome sets. Virtually all sexually reproducing organisms are made up of somatic cells that are diploid or greater, but ploidy level may vary widely between different organisms, between different tissues within the same organism, and at different stages in an organism's life cycle. Half ...
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In Vitro
''In vitro'' (meaning in glass, or ''in the glass'') studies are performed with microorganisms, cells, or biological molecules outside their normal biological context. Colloquially called "test-tube experiments", these studies in biology and its subdisciplines are traditionally done in labware such as test tubes, flasks, Petri dishes, and microtiter plates. Studies conducted using components of an organism that have been isolated from their usual biological surroundings permit a more detailed or more convenient analysis than can be done with whole organisms; however, results obtained from ''in vitro'' experiments may not fully or accurately predict the effects on a whole organism. In contrast to ''in vitro'' experiments, ''in vivo'' studies are those conducted in living organisms, including humans, and whole plants. Definition ''In vitro'' ( la, in glass; often not italicized in English usage) studies are conducted using components of an organism that have been isolated fro ...
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Backcross Conversion
Backcrossing is a crossing of a hybrid with one of its parents or an individual genetically similar to its parent, to achieve offspring with a genetic identity closer to that of the parent. It is used in horticulture, animal breeding, and production of gene knockout organisms. Backcrossed hybrids are sometimes described with acronym "BC"; for example, an F1 hybrid crossed with one of its parents (or a genetically similar individual) can be termed a BC1 hybrid, and a further cross of the BC1 hybrid to the same parent (or a genetically similar individual) produces a BC2 hybrid. Plants Advantages * If the recurrent parent is an elite genotype, at the end of the backcrossing programme, an elite genotype is recovered. * As no "new" recombination results, the elite combination is not lost. Disadvantages * It works poorly for quantitative traits. * It is more restricted for recessive traits. * In practice, sections of genome from the nonrecurrent parents are often still present and c ...
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Phenotyping
In genetics, the phenotype () is the set of observable characteristics or traits of an organism. The term covers the organism's morphology or physical form and structure, its developmental processes, its biochemical and physiological properties, its behavior, and the products of behavior. An organism's phenotype results from two basic factors: the expression of an organism's genetic code, or its genotype, and the influence of environmental factors. Both factors may interact, further affecting phenotype. When two or more clearly different phenotypes exist in the same population of a species, the species is called polymorphic. A well-documented example of polymorphism is Labrador Retriever coloring; while the coat color depends on many genes, it is clearly seen in the environment as yellow, black, and brown. Richard Dawkins in 1978 and then again in his 1982 book ''The Extended Phenotype'' suggested that one can regard bird nests and other built structures such as caddis ...
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Quantitative Trait Loci
A quantitative trait locus (QTL) is a locus (section of DNA) that correlates with variation of a quantitative trait in the phenotype of a population of organisms. QTLs are mapped by identifying which molecular markers (such as SNPs or AFLPs) correlate with an observed trait. This is often an early step in identifying the actual genes that cause the trait variation. Definition A quantitative trait locus (QTL) is a region of DNA which is associated with a particular phenotypic trait, which varies in degree and which can be attributed to polygenic effects, i.e., the product of two or more genes, and their environment. . These QTLs are often found on different chromosomes. The number of QTLs which explain variation in the phenotypic trait indicates the genetic architecture of a trait. It may indicate that plant height is controlled by many genes of small effect, or by a few genes of large effect. Typically, QTLs underlie continuous traits (those traits which vary continuously, ...
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F1 Hybrid
An F1 hybrid (also known as filial 1 hybrid) is the first filial generation of offspring of distinctly different parental types. F1 hybrids are used in genetics, and in selective breeding, where the term F1 crossbreed may be used. The term is sometimes written with a subscript, as F hybrid. Subsequent generations are called F, F, etc. The offspring of distinctly different parental types produce a new, uniform phenotype with a combination of characteristics from the parents. In fish breeding, those parents frequently are two closely related fish species, while in plant and animal breeding, the parents often are two inbred lines. Gregor Mendel focused on patterns of inheritance and the genetic basis for variation. In his cross-pollination experiments involving two true-breeding, or homozygous, parents, Mendel found that the resulting F1 generation was heterozygous and consistent. The offspring showed a combination of the phenotypes from each parent that were genetically dominant. ...
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Offspring
In biology, offspring are the young creation of living organisms, produced either by a single organism or, in the case of sexual reproduction, two organisms. Collective offspring may be known as a brood or progeny in a more general way. This can refer to a set of simultaneous offspring, such as the chicks hatched from one clutch of eggs, or to all the offspring, as with the honeybee. Human offspring ( descendants) are referred to as children (without reference to age, thus one can refer to a parent's " minor children" or " adult children" or " infant children" or " teenage children" depending on their age); male children are sons and female children are daughters (see kinship). Offspring can occur after mating or after artificial insemination. Offspring contains many parts and properties that are precise and accurate in what they consist of, and what they define. As the offspring of a new species, also known as a child or f1 generation, consist of genes of the father and the ...
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Nicotiana Africana
''Nicotiana africana'' is a species of plant in the family Solanaceae. It is endemic to Namibia. Its natural habitat In ecology, the term habitat summarises the array of resources, physical and biotic factors that are present in an area, such as to support the survival and reproduction of a particular species. A species habitat can be seen as the physical ...s are subtropical or tropical dry shrubland and rocky areas. References Endemic flora of Namibia africana Least concern plants Taxonomy articles created by Polbot {{Solanales-stub ...
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Nicotiana Tabacum
''Nicotiana tabacum'', or cultivated tobacco, is an annually grown herbaceous plant of the ''Nicotiana'' genus. The plant is tropical in origin, is commonly grown throughout the world, and is often found in cultivation. It grows to heights between 1 and 2 meters (3' to 6'). Research is ongoing into its ancestry among wild ''Nicotiana'' species, but it is believed to be a hybrid of ''Nicotiana sylvestris'', '' Nicotiana tomentosiformis'', and possibly '' Nicotiana otophora''. It is the most commonly grown of all plants in the genus ''Nicotiana,'' the plants' leaves commercially grown to be processed into tobacco. Description It is an annual plant that grows high and is sticky hairy on all parts. The stems are thick and not very branched. The leaves can be over long with the blades ovate to elliptical, or obovate, pointed towards the front and, at the base, run down the stem or are sessile, encompassing the stem. The scented inflorescences are multi-branched panicles. The flow ...
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Hordeum Bulbosum
''Hordeum bulbosum'', bulbous barley, is a species of barley native to southern Europe, northern Africa, the Middle East and as far east as Afghanistan, with a few naturalized populations in North America, South America and Australia. Since 1970 it has been used in the ''Hordeum bulbosum'' Method (or Technique) to produce doubled haploid (DH) wheat and barley Barley (''Hordeum vulgare''), a member of the grass family, is a major cereal grain grown in temperate climates globally. It was one of the first cultivated grains, particularly in Eurasia as early as 10,000 years ago. Globally 70% of barley p ... plants by crossing it with ''T.aestivum'' or ''H.vulgare'', followed by the elimination of the ''H.bulbosum'' chromosomes from the offspring. These DH plants are important in breeding new varieties of wheat and barley, and in scientific studies. ''H. bulbosum'' is also being looked at as a source of genes for disease resistance and other traits for barley crop improvement. Re ...
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Androgenesis
Androgenesis occurs when a zygote is produced with only paternal nuclear genes. During standard sexual reproduction, one female and one male parent each produce haploid gametes(such as a sperm or egg cell, each containing only a single set of chromosomes), which recombine to create offspring with genetic material from both parents. However, in androgenesis, there is no recombination of maternal and paternal chromosomes, and only the paternal chromosomes are passed down to the offspring (the inverse of this is gynogenesis, where only the maternal chromosomes are inherited, which is more common than androgenesis). The offspring produced in androgenesis will still have maternally inherited mitochondria, as is the case with most sexually reproducing species. One of two things can occur to produce offspring with exclusively paternal genetic material: the maternal nuclear genome can be eliminated from the zygote, or the female can produce an egg with no nucleus, resulting in an embryo d ...
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Ovary
The ovary is an organ in the female reproductive system that produces an ovum. When released, this travels down the fallopian tube into the uterus, where it may become fertilized by a sperm. There is an ovary () found on each side of the body. The ovaries also secrete hormones that play a role in the menstrual cycle and fertility. The ovary progresses through many stages beginning in the prenatal period through menopause. It is also an endocrine gland because of the various hormones that it secretes. Structure The ovaries are considered the female gonads. Each ovary is whitish in color and located alongside the lateral wall of the uterus in a region called the ovarian fossa. The ovarian fossa is the region that is bounded by the external iliac artery and in front of the ureter and the internal iliac artery. This area is about 4 cm x 3 cm x 2 cm in size.Daftary, Shirish; Chakravarti, Sudip (2011). Manual of Obstetrics, 3rd Edition. Elsevier. pp. 1-16. . The ovari ...
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