Cryopreserved
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Cryopreserved
Cryo-preservation or cryo-conservation is a process where organisms, organelles, cells, tissues, extracellular matrix, organs, or any other biological constructs susceptible to damage caused by unregulated chemical kinetics are preserved by cooling to very low temperatures (typically using solid carbon dioxide or using liquid nitrogen). At low enough temperatures, any enzymatic or chemical activity which might cause damage to the biological material in question is effectively stopped. Cryopreservation methods seek to reach low temperatures without causing additional damage caused by the formation of ice crystals during freezing. Traditional cryopreservation has relied on coating the material to be frozen with a class of molecules termed cryoprotectants. New methods are being investigated due to the inherent toxicity of many cryoprotectants. Cryoconservation of animal genetic resources is done with the intention of conservation of the breed. Natural cryopreservation Tardigra ...
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Cryopreservation USDA Gene Bank
Cryo-preservation or cryo-conservation is a process where organisms, organelles, cells, tissues, extracellular matrix, organs, or any other biological constructs susceptible to damage caused by unregulated chemical kinetics are preserved by cooling to very low temperatures (typically using solid carbon dioxide or using liquid nitrogen). At low enough temperatures, any enzymatic or chemical activity which might cause damage to the biological material in question is effectively stopped. Cryopreservation methods seek to reach low temperatures without causing additional damage caused by the formation of ice crystals during freezing. Traditional cryopreservation has relied on coating the material to be frozen with a class of molecules termed cryoprotectants. New methods are being investigated due to the inherent toxicity of many cryoprotectants. Cryoconservation of animal genetic resources is done with the intention of conservation of the breed. Natural cryopreservation Tardigr ...
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Cryoconservation Of Animal Genetic Resources
Cryoconservation of animal genetic resources is a strategy wherein samples of animal genetic materials are preserved cryogenically."Cryoconservation of Animal Genetic Resources", Rep. Rome: Food and Agriculture Organization of the United Nations, 2012. FAO Animal Production and Health Guidelines No. 12. Print. Animal genetic resources, as defined by the Food and Agriculture Organization of the United Nations, are "those animal species that are used, or may be used, for the production of food and agriculture, and the populations within each of them. These populations within each species can be classified as wild and feral populations, landraces and primary populations, standardised breeds, selected lines, varieties, strains and any conserved genetic material; all of which are currently categorized as Breeds." Genetic materials that are typically cryogenically preserved include sperm, oocytes, embryos and somatic cells.Mazur, P., S.p Leibo, and G. E. Seidel.Cryopreservation of th ...
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Tardigrade
Tardigrades (), known colloquially as water bears or moss piglets, are a phylum of eight-legged segmented micro-animals. They were first described by the German zoologist Johann August Ephraim Goeze in 1773, who called them Kleiner Wasserbär ("little water bear"). In 1777, the Italian biologist Lazzaro Spallanzani named them Tardigrada (), which means "slow steppers". They have been found in diverse regions of Earth's biospheremountaintops, the deep sea, tropical rainforests, and the Antarctic. Tardigrades are among the most resilient animals known, with individual species able to survive extreme conditions – such as exposure to extreme temperatures, extreme pressures (both high and low), air deprivation, radiation, dehydration, and starvation – that would quickly kill most other known forms of life. Tardigrades have survived exposure to outer space. There are about 1,300 known species in the phylum Tardigrada, a part of the superphylum Ecdysozoa consisting of animals th ...
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Trehalose
Trehalose (from Turkish '' tıgala'' – a sugar derived from insect cocoons + -ose) is a sugar consisting of two molecules of glucose. It is also known as mycose or tremalose. Some bacteria, fungi, plants and invertebrate animals synthesize it as a source of energy, and to survive freezing and lack of water. Extracting trehalose was once a difficult and costly process, but around 2000, the Hayashibara company ( Okayama, Japan) discovered an inexpensive extraction technology from starch. Trehalose has high water retention capabilities, and is used in food, cosmetics and as a drug. A procedure developed in 2017 using trehalose allows sperm storage at room temperatures. Structure Trehalose is a disaccharide formed by a bond between two α-glucose units. It is found in nature as a disaccharide and also as a monomer in some polymers. Two other isomers exist, α,β-trehalose, otherwise known as neotrehalose, and β,β-trehalose (also referred to as isotrehalose). Neotrehalose has ...
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Terrapene Carolina
The common box turtle (''Terrapene carolina'') is a species of box turtle with six existing subspecies. It is found throughout the Eastern United States and Mexico. The box turtle has a distinctive hinged lowered shell (the box) that allows it to completely enclose itself. Its upper jaw is long and curved. The turtle is primarily terrestrial and eats a wide variety of plants and animals. The females lay their eggs in the summer. Turtles in the northern part of their range hibernate over the winter. Common box turtle numbers are declining because of habitat loss, roadkill, and capture for the pet trade. The species is classified as vulnerable to threats to its survival by the IUCN Red List. Three U.S. states name subspecies of the common box turtle as their official reptile. Classification ''Terrapene carolina'' was first described by Carl Linnaeus in his landmark 1758 10th edition of ''Systema Naturae''. It is the type species for the genus ''Terrapene'' and has more subs ...
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Chrysemys Picta
The painted turtle (''Chrysemys picta'') is the most widespread native turtle of North America. It lives in slow-moving fresh waters, from southern Canada to northern Mexico, and from the Atlantic to the Pacific. They have been shown to prefer large wetlands with long periods of inundation and emergent vegetation. This turtle is a member of the genus ''Chrysemys'', which is part of the pond turtle family Emydidae. Fossils show that the painted turtle existed 15 million years ago. Three regionally based subspecies (the eastern, midland, and western) evolved during the last ice age. The southern painted turtle (''C. dorsalis'') is alternately considered the only other species in ''Chrysemys'', or another subspecies of ''C. picta''. The adult painted turtle is 13–25 cm (5–10 in) long; the male is smaller than the female. The turtle's top shell is dark and smooth, without a ridge. Its skin is olive to black with red, orange, or yellow stripes on its extremities. T ...
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Thamnophis Sirtalis
The common garter snake (''Thamnophis sirtalis'') is a species of thamnophis snake, in the natricine subfamily, which is indigenous to North America and found widely across the continent. Most common garter snakes have a pattern of yellow stripes on a black, brown or green background, and their average total length (including tail) is about , with a maximum total length of about . The average body mass is . Common garter snakes are the state reptile of Massachusetts. Description Common garter snakes are thin snakes. Few grow over about long, and most stay smaller. Most have longitudinal stripes in many different colors. Common garter snakes come in a wide range of colors, including green, blue, yellow, gold, red, orange, brown, and black. Life history The common garter snake is a diurnal snake. In summer, it is most active in the morning and late afternoon; in cooler seasons or climates, it restricts its activity to the warm afternoons. In warmer southern areas, the snake ...
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Salamandrella Keyserlingii
''Salamandrella keyserlingii'', the Siberian salamander, is a species of salamander found in Northeast Asia. It lives in wet woods and riparian groves. Distribution It is found primarily in Siberia east of the Sosva River and the Urals, in the East Siberian Mountains, including the Verkhoyansk Range, northeast to the Anadyr Highlands, east to the Kamchatka Peninsula and south into Manchuria, with outlying populations also in northern Kazakhstan and Mongolia, northeastern China, and on the Korean Peninsula. It is believed to be extirpated from South Korea South Korea, officially the Republic of Korea (ROK), is a country in East Asia, constituting the southern part of the Korean Peninsula and sharing a land border with North Korea. Its western border is formed by the Yellow Sea, while its eas .... An isolated population exists on Hokkaidō, Japan, in the Kushiro Shitsugen National Park. A breeding ground of Siberian salamanders in Paegam, South Hamgyong, is des ...
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Hyla Chrysoscelis
Cope's gray treefrog (''Dryophytes chrysoscelis'') is a species of treefrog found in the United States. It is almost indistinguishable from the gray treefrog ('' Dryophytes versicolor''), and shares much of its geographic range. Both species are variable in color, mottled gray to gray-green, resembling the bark of trees. These are treefrogs of woodland habitats, though they will sometimes travel into more open areas to reach a breeding pond. The only readily noticeable difference between the two species is the mating call — Cope's has a faster-paced and slightly higher-pitched call than ''D. versicolor''. In addition, ''D. chrysoscelis'' is reported to be slightly smaller, more arboreal, and more tolerant of dry conditions than ''D. versicolor''.Martof, B. S., et al. (1980). ''Amphibians and Reptiles of the Carolinas and Virginia''. Chapel Hill: University of North Carolina Press. . Taxonomy Edward Drinker Cope described the species in 1880. The specific name, ''chrysoscelis' ...
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Hyla Versicolor
The gray treefrog (''Dryophytes versicolor'') is a species of small arboreal holarctic tree frog native to much of the eastern United States and southeastern Canada. It is sometimes referred to as the eastern gray treefrog, northern gray treefrog, common gray treefrog, or tetraploid gray treefrog to distinguish it from its more southern, genetically disparate relative, Cope's gray treefrog. Description As the scientific name implies, gray treefrogs are variable in color. This ability to vary their color provides them with the ability to camouflage themselves from gray to green or brown, depending on the environment around them. ''H. versicolor'' can change from nearly black to nearly white. They change color at a slower rate than a chameleon. A unique aspect of the appearance of gray treefrogs is that its legs feature a dark band-like pattern which then contrast sharply with the black-marked bright yellow or orange under the sides of its legs and arms. Dead gray treefrogs and ...
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Hyla Crucifer
The spring peeper (''Pseudacris crucifer'') is a small chorus frog widespread throughout the eastern United States and Canada. They prefer permanent ponds due to their advantage in avoiding predation; however, they are very adaptable with respect to the habitat they can live in. Due to their presence in northern regions, the frog is able to endure below freezing temperatures. They are so called because of their chirping call that marks the beginning of spring. ''Crucifer'' is derived from the Latin root meaning "cross-bearing." This could be a reference to the cross-like pattern on the spring peeper's dorsal side. These chirping calls are significant for communication in mating as females choose their mates based on the frequency and volume associated with them. Satellite males who do not make any calls also strategically place themselves near those that make louder calls in an attempt to intercept females. Temperature plays a large role in when the spring peeper begins br ...
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