|
Critical Illumination
Critical illumination or Nelsonian illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy. Critical illumination focuses an image of a light source on to the specimen for bright illumination. Critical illumination generally has problems with evenness of illumination as an image of the illumination source (for example a halogen lamp filament) is visible in the resulting image. Köhler illumination has largely replaced critical illumination in modern scientific light microscopy although it requires additional optics which may not be present in less expensive and simpler light microscopes. Optical principles Critical illumination acts to form an image of the light source on the specimen to illuminate it. This image is formed by the condenser or collector lens. This illumination is bright but not always even, as any structure in the light source (for example the filament of a light bulb) will be visi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Laboratory Specimen
Need In medicine, a laboratory specimen is a biological specimen of a medical patient's tissue, fluids, or other material used for laboratory analysis to assist in differential diagnosis or staging of a disease process. For example, to detect breast cancer, the breast tissue is biopsied, and the extracted specimen is sent to a lab for analysis and testing. This method of testing often yields extremely high levels of accuracy, with a reported 1-2% of cases having incorrect biopsy results General areas for cellular tissue extraction: * Bone marrow aspiration * Cardiac * Core * Endometrial biopsy * Endoscopic biopsy * Excisional and incisional * Fine-needle aspiration * Lymph node Preparation After extraction, all specimen containers are required to be labeled with at least two of the following identifiers (at the time of collection): patient's name, date of birth, hospital number, test request form number, accession number, or unique random number. Afterwards, all specimen ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Illumination (lighting)
Lighting or illumination is the deliberate use of light to achieve practical or aesthetic effects. Lighting includes the use of both artificial light sources like lamps and light fixtures, as well as natural illumination by capturing daylight. Daylighting (using windows, skylights, or light shelves) is sometimes used as the main source of light during daytime in buildings. This can save energy in place of using artificial lighting, which represents a major component of energy consumption in buildings. Proper lighting can enhance task performance, improve the appearance of an area, or have positive psychological effects on occupants. Indoor lighting is usually accomplished using light fixtures, and is a key part of interior design. Lighting can also be an intrinsic component of landscape projects. History With the discovery of fire, the earliest form of artificial lighting used to illuminate an area were campfires or torches. As early as 400,000 years ago, fire was kindled ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Optical Microscopy
Optics is the branch of physics that studies the behaviour and properties of light, including its interactions with matter and the construction of instruments that use or detect it. Optics usually describes the behaviour of visible, ultraviolet, and infrared light. Because light is an electromagnetic wave, other forms of electromagnetic radiation such as X-rays, microwaves, and radio waves exhibit similar properties. Most optical phenomena can be accounted for by using the classical electromagnetic description of light. Complete electromagnetic descriptions of light are, however, often difficult to apply in practice. Practical optics is usually done using simplified models. The most common of these, geometric optics, treats light as a collection of rays that travel in straight lines and bend when they pass through or reflect from surfaces. Physical optics is a more comprehensive model of light, which includes wave effects such as diffraction and interference that cannot be ac ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Köhler Illumination
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy. Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image. Köhler illumination is the predominant technique for sample illumination in modern scientific light microscopy. It requires additional optical elements which are more expensive and may not be present in more basic light microscopes. History and motivation Prior to Köhler illumination critical illumination was the predominant technique for sample illumination. Critical illumination has the major limitation that the image of the light source (typically a light bulb) falls in the same plane as the image of the specimen, i.e. the bulb filament is visible in the final image. The image of the light source is often referred to as the filament ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Critical Illumination
Critical illumination or Nelsonian illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy. Critical illumination focuses an image of a light source on to the specimen for bright illumination. Critical illumination generally has problems with evenness of illumination as an image of the illumination source (for example a halogen lamp filament) is visible in the resulting image. Köhler illumination has largely replaced critical illumination in modern scientific light microscopy although it requires additional optics which may not be present in less expensive and simpler light microscopes. Optical principles Critical illumination acts to form an image of the light source on the specimen to illuminate it. This image is formed by the condenser or collector lens. This illumination is bright but not always even, as any structure in the light source (for example the filament of a light bulb) will be visi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Condenser (optics)
A condenser is an optical lens which renders a divergent beam from a point source into a parallel or converging beam to illuminate an object. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes. The concept is applicable to all kinds of radiation undergoing optical transformation, such as electrons in electron microscopy, neutron radiation and synchrotron radiation optics. Microscope condenser Condensers are located above the light source and under the sample in an upright microscope, and above the stage and below the light source in an inverted microscope. They act to gather light from the microscope's light source and concentrate it into a cone of light that illuminates the specimen. The aperture and angle of the light cone must be adjusted (via the size of the diaphragm) for each different objective lens with different numerical apertures. Condensers typically consist of a variable-aperture diaphragm and ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Light Bulb
An electric light, lamp, or light bulb is an electrical component that produces light. It is the most common form of artificial lighting. Lamps usually have a base made of ceramic, metal, glass, or plastic, which secures the lamp in the socket of a light fixture, which is often called a "lamp" as well. The electrical connection to the socket may be made with a screw-thread base, two metal pins, two metal caps or a bayonet cap. The three main categories of electric lights are incandescent lamps, which produce light by a filament heated white-hot by electric current, gas-discharge lamps, which produce light by means of an electric arc through a gas, such as fluorescent lamps, and LED lamps, which produce light by a flow of electrons across a band gap in a semiconductor. Before electric lighting became common in the early 20th century, people used candles, gas lights, oil lamps, and fires. Vasily Vladimirovich Petrov developed the first persistent electric arc in 1802, and ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Flame
A flame (from Latin ''flamma'') is the visible, gaseous part of a fire. It is caused by a highly exothermic chemical reaction taking place in a thin zone. When flames are hot enough to have ionized gaseous components of sufficient density they are then considered plasma. Mechanism Color and temperature of a flame are dependent on the type of fuel involved in the combustion, as, for example, when a lighter is held to a candle. The applied heat causes the fuel molecules in the candle wax to vaporize (if this process happens in inert atmosphere without oxidizer, it is called pyrolysis). In this state they can then readily react with oxygen in the air, which gives off enough heat in the subsequent exothermic reaction to vaporize yet more fuel, thus sustaining a consistent flame. The high temperature of the flame causes the vaporized fuel molecules to decompose, forming various incomplete combustion products and free radicals, and these products then react with each other and with ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sunlight
Sunlight is a portion of the electromagnetic radiation given off by the Sun, in particular infrared, visible, and ultraviolet light. On Earth, sunlight is scattered and filtered through Earth's atmosphere, and is obvious as daylight when the Sun is above the horizon. When direct solar radiation is not blocked by clouds, it is experienced as sunshine, a combination of bright light and radiant heat. When blocked by clouds or reflected off other objects, sunlight is diffused. Sources estimate a global average of between 164 watts to 340 watts per square meter over a 24-hour day; this figure is estimated by NASA to be about a quarter of Earth's average total solar irradiance. The ultraviolet radiation in sunlight has both positive and negative health effects, as it is both a requisite for vitamin D3 synthesis and a mutagen. Sunlight takes about 8.3 minutes to reach Earth from the surface of the Sun. A photon starting at the center of the Sun and changing direction eve ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ground Glass
Ground glass is glass whose surface has been ground to produce a flat but rough (matte) finish, in which the glass is in small sharp fragments. Ground glass surfaces have many applications, ranging from ornamentation on windows and table glassware to scientific uses in optics and laboratory glassware. Uses Photography In photography, a sheet of ground glass is used for the manual focusing in some still and movie cameras; the ground-glass viewer is inserted in the back of the camera, and the lens opened to its widest aperture. This projects the scene on the ground glass upside down. The photographer focuses and composes using this projected image, sometimes with the aid of a magnifying glass (or loupe). In order to see the image better, a dark cloth is used to block out light, whence came the image of the old-time photographer with his head stuck under a large black cloth. A ground glass is also used in the reflex finder of an SLR or TLR camera. In motion-picture cameras, t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Milk Glass
Milk glass is an opaque or translucent, milk white or colored glass that can be blown or pressed into a wide variety of shapes. First made in Venice in the 16th century, colors include blue, pink, yellow, brown, black, and white. Principle Milk glass contains dispersion of particles with refractive index significantly different from the glass matrix, which light scattering, scatter light by the Tyndall scattering mechanism. The size distribution and density of the particles control the overall effect, which may range from mild opalization to opaque white. Some glasses are somewhat more blue from the side, and somewhat red-orange in pass-through light. The particles are produced via addition of opacifiers to the melt. Some opacifiers can be insoluble and only dispersed in the melt. Others are added as precursors and react in the melt, or dissolve in the molten glass and then precipitate as crystals on cooling; this is similar to color production in Glass coloring and color mark ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Optical Microscopy
Optics is the branch of physics that studies the behaviour and properties of light, including its interactions with matter and the construction of instruments that use or detect it. Optics usually describes the behaviour of visible, ultraviolet, and infrared light. Because light is an electromagnetic wave, other forms of electromagnetic radiation such as X-rays, microwaves, and radio waves exhibit similar properties. Most optical phenomena can be accounted for by using the classical electromagnetic description of light. Complete electromagnetic descriptions of light are, however, often difficult to apply in practice. Practical optics is usually done using simplified models. The most common of these, geometric optics, treats light as a collection of rays that travel in straight lines and bend when they pass through or reflect from surfaces. Physical optics is a more comprehensive model of light, which includes wave effects such as diffraction and interference that cannot be ac ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
