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CpDNA
Chloroplast DNA (cpDNA) is the DNA located in chloroplasts, which are photosynthetic organelles located within the cells of some eukaryotic organisms. Chloroplasts, like other types of plastid, contain a genome separate from that in the cell nucleus. The existence of chloroplast DNA was identified biochemically in 1959, and confirmed by electron microscopy in 1962. The discoveries that the chloroplast contains ribosomes and performs protein synthesis revealed that the chloroplast is genetically semi-autonomous. The first complete chloroplast genome sequences were published in 1986, ''Nicotiana tabacum'' (tobacco) by Sugiura and colleagues and ''Marchantia polymorpha'' (liverwort) by Ozeki et al. Since then, a great number of chloroplast DNAs from various species have been sequenced. Molecular structure Chloroplast DNAs are circular, and are typically 120,000–170,000 base pairs long. They can have a contour length of around 30–60 micrometers, and have a mass of about 80–1 ...
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Chloroplast
A chloroplast () is a type of membrane-bound organelle known as a plastid that conducts photosynthesis mostly in plant and algal cells. The photosynthetic pigment chlorophyll captures the energy from sunlight, converts it, and stores it in the energy-storage molecules ATP and NADPH while freeing oxygen from water in the cells. The ATP and NADPH is then used to make organic molecules from carbon dioxide in a process known as the Calvin cycle. Chloroplasts carry out a number of other functions, including fatty acid synthesis, amino acid synthesis, and the immune response in plants. The number of chloroplasts per cell varies from one, in unicellular algae, up to 100 in plants like '' Arabidopsis'' and wheat. A chloroplast is characterized by its two membranes and a high concentration of chlorophyll. Other plastid types, such as the leucoplast and the chromoplast, contain little chlorophyll and do not carry out photosynthesis. Chloroplasts are highly dynamic—they cir ...
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List Of Sequenced Plastomes
A plastome is the genome of a plastid, a type of organelle found in plants and in a variety of protoctists. The number of known plastid genome sequences grew rapidly in the first decade of the twenty-first century. For example, 25 chloroplast genomes were sequenced for one molecular phylogenetic study. The flowering plants are especially well represented in complete chloroplast genomes. As of January, 2017, all of their orders are represented except Commelinales, Picramniales, Huerteales, Escalloniales, Bruniales, and Paracryphiales. A compilation of all available complete plastid genomes is maintained by the NCBI in a public repository. Plants Bryophytes ''s.l.'' Ferns and Lycophytes Gymnosperms Flowering plants This sortable table is expected to compile complete plastid genomes representing the largest range of sizes, number of genes, and angiosperm families. Green algae Red algae Glaucophytes Meta-algae and apicomplexans Meta-algae are organisms with pho ...
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Nicotiana Tabacum
''Nicotiana tabacum'', or cultivated tobacco, is an Annual plant, annually grown herbaceous plant of the ''Nicotiana'' genus. The plant is tropical in origin, is commonly grown throughout the world, and is often found in Cultivation of tobacco, cultivation. It grows to heights between 1 and 2 meters (3' to 6'). Research is ongoing into its ancestry among wild ''Nicotiana'' species, but it is believed to be a hybrid of ''Nicotiana sylvestris'', ''Nicotiana tomentosiformis'', and possibly ''Nicotiana otophora''. It is the most commonly grown of all plants in the genus ''Nicotiana,'' the plants' leaves commercially grown to be processed into tobacco. Description It is an annual plant that grows high and is sticky hairy on all parts. The stems are thick and not very branched. The leaves can be over long with the leaf blade, blades ovate to elliptical, or obovate, pointed towards the front and, at the base, run down the stem or are sessility (botany), sessile, encompassing the stem. ...
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Genome Reduction
Genome size is the total amount of DNA contained within one copy of a single complete genome. It is typically measured in terms of mass in picograms (trillionths (10−12) of a gram, abbreviated pg) or less frequently in daltons, or as the total number of nucleotide base pairs, usually in megabases (millions of base pairs, abbreviated Mb or Mbp). One picogram is equal to 978 megabases. In diploid organisms, genome size is often used interchangeably with the term C-value. An organism's complexity is not directly proportional to its genome size; total DNA content is widely variable between biological taxa. Some single-celled organisms have much more DNA than humans, for reasons that remain unclear (see non-coding DNA and C-value enigma). Origin of the term The term "genome size" is often erroneously attributed to a 1976 paper by Ralph Hinegardner, even in discussions dealing specifically with terminology in this area of research (e.g., Greilhuber 2005). Notably, Hinegardner us ...
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Endosymbiont
An ''endosymbiont'' or ''endobiont'' is any organism that lives within the body or cells of another organism most often, though not always, in a mutualistic relationship. (The term endosymbiosis is from the Greek: ἔνδον ''endon'' "within", σύν ''syn'' "together" and βίωσις ''biosis'' "living".) Examples are nitrogen-fixing bacteria (called rhizobia), which live in the root nodules of legumes, single-cell algae inside reef-building corals and bacterial endosymbionts that provide essential nutrients to insects. There are two types of symbiont transmissions. In horizontal transmission, each new generation acquires free living symbionts from the environment. An example is the nitrogen-fixing bacteria in certain plant roots. Vertical transmission takes place when the symbiont is transferred directly from parent to offspring. It is also possible for both to be involved in a mixed-mode transmission, where symbionts are transferred vertically for some generation befor ...
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Stroma (fluid)
Stroma, in botany, refers to the colorless fluid surrounding the grana within the chloroplast. Within the stroma are grana (stacks of thylakoid), and the sub-organelles or daughter cells, where photosynthesis is commenced before the chemical changes are completed in the stroma. Kramer & Scott ''flower'' iv. 80 1979 Photosynthesis occurs in two stages. In the first stage, ''light-dependent reactions'' capture the energy of light and use it to make the energy-storage molecules ATP and NADPH. During the second stage, the '' light-independent reactions'' use these products to fix carbon by capturing and reducing carbon dioxide. The series of biochemical redox reactions which take place in the stroma are collectively called the Calvin cycle or '' light-independent reactions''. There are three phases: carbon fixation, reduction reactions, and ribulose 1,5-bisphosphate (RuBP) regeneration. The stroma is also the location of chloroplast DNA and chloroplast ribosomes, and thus a ...
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Green Algae
The green algae (singular: green alga) are a group consisting of the Prasinodermophyta and its unnamed sister which contains the Chlorophyta and Charophyta/ Streptophyta. The land plants (Embryophytes) have emerged deep in the Charophyte alga as sister of the Zygnematophyceae. Since the realization that the Embryophytes emerged within the green algae, some authors are starting to properly include them. The completed clade that includes both green algae and embryophytes is monophyletic and is referred to as the clade Viridiplantae and as the kingdom Plantae. The green algae include unicellular and colonial flagellates, most with two flagella per cell, as well as various colonial, coccoid and filamentous forms, and macroscopic, multicellular seaweeds. There are about 22,000 species of green algae. Many species live most of their lives as single cells, while other species form coenobia (colonies), long filaments, or highly differentiated macroscopic seaweeds. A few other org ...
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Nucleoid
The nucleoid (meaning ''nucleus-like'') is an irregularly shaped region within the prokaryotic cell that contains all or most of the genetic material. The chromosome of a prokaryote is circular, and its length is very large compared to the cell dimensions, so it needs to be compacted in order to fit. In contrast to the nucleus of a eukaryotic cell, it is not surrounded by a nuclear membrane. Instead, the nucleoid forms by condensation and functional arrangement with the help of chromosomal architectural proteins and RNA molecules as well as DNA supercoiling. The length of a genome widely varies (generally at least a few million base pairs) and a cell may contain multiple copies of it. There is not yet a high-resolution structure known of a bacterial nucleoid, however key features have been researched in ''Escherichia coli'' as a model organism. In ''E. coli'', the chromosomal DNA is on average negatively supercoiled and folded into plectonemic loops, which are confined to d ...
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Histone
In biology, histones are highly basic proteins abundant in lysine and arginine residues that are found in eukaryotic cell nuclei. They act as spools around which DNA winds to create structural units called nucleosomes. Nucleosomes in turn are wrapped into 30- nanometer fibers that form tightly packed chromatin. Histones prevent DNA from becoming tangled and protect it from DNA damage. In addition, histones play important roles in gene regulation and DNA replication. Without histones, unwound DNA in chromosomes would be very long. For example, each human cell has about 1.8 meters of DNA if completely stretched out; however, when wound about histones, this length is reduced to about 90 micrometers (0.09 mm) of 30 nm diameter chromatin fibers. There are five families of histones which are designated H1/H5 (linker histones), H2, H3, and H4 (core histones). The nucleosome core is formed of two H2A-H2B dimers and a H3-H4 tetramer. The tight wrapping of DNA around h ...
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Nucleoids
The nucleoid (meaning ''nucleus-like'') is an irregularly shaped region within the prokaryotic cell that contains all or most of the genetic material. The chromosome of a prokaryote is circular, and its length is very large compared to the cell dimensions, so it needs to be compacted in order to fit. In contrast to the nucleus of a eukaryotic cell, it is not surrounded by a nuclear membrane. Instead, the nucleoid forms by condensation and functional arrangement with the help of chromosomal architectural proteins and RNA molecules as well as DNA supercoiling. The length of a genome widely varies (generally at least a few million base pairs) and a cell may contain multiple copies of it. There is not yet a high-resolution structure known of a bacterial nucleoid, however key features have been researched in ''Escherichia coli'' as a model organism. In ''E. coli'', the chromosomal DNA is on average negatively supercoiled and folded into plectonemic loops, which are confined to dif ...
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Porphyra
''Porphyra'' is a genus of coldwater seaweeds that grow in cold, shallow seawater. More specifically, it belongs to red algae phylum of laver species (from which comes laverbread), comprising approximately 70 species.Brodie, J.A. and Irvine, L.M. 2003. ''Seaweeds of the British Isles.'' Volume 1 Part 3b. The Natural History Museum, London. It grows in the intertidal zone, typically between the upper intertidal zone and the splash zone in cold waters of temperate oceans. In East Asia, it is used to produce the sea vegetable products '' nori'' (in Japan) and '' gim'' (in Korea). There are considered to be 60 to 70 species of ''Porphyra'' worldwide Kain, J.M. 1991. Cultivation of attached seaweeds. in Guiry, M.D. and Blunden, G. 1992. ''Seaweed Resources in Europe: Uses and Potential.'' John Wiley and Sons, Chichester and seven around Britain and Ireland where it has been traditionally used to produce edible sea vegetables on the Irish Sea coast.Hardy, F.G. and Guiry, M.D. 2006. ...
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Peas
The pea is most commonly the small spherical seed or the seed-pod of the flowering plant species ''Pisum sativum''. Each pod contains several peas, which can be green or yellow. Botanically, pea pods are fruit, since they contain seeds and develop from the ovary of a (pea) flower. The name is also used to describe other edible seeds from the Fabaceae such as the pigeon pea (''Cajanus cajan''), the cowpea (''Vigna unguiculata''), and the seeds from several species of ''Lathyrus''. Peas are annual plants, with a life cycle of one year. They are a cool-season crop grown in many parts of the world; planting can take place from winter to early summer depending on location. The average pea weighs between 0.1 and 0.36 gram. The immature peas (and in snow peas the tender pod as well) are used as a vegetable, fresh, frozen or canned; varieties of the species typically called field peas are grown to produce dry peas like the split pea shelled from a matured pod. These are the ba ...
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