Brain-heart Infusion
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Brain-heart Infusion
Brain heart infusion (BHI) is a growth medium for growing microorganisms. It is a nutrient-rich medium, and can therefore be used to culture a variety of fastidious organisms. In particular, it has been used to culture streptococci, pneumococci and meningococci, which can be otherwise challenging to grow. BHI is made by combining an infusion from boiled bovine or porcine heart and brain with a variety of other nutrients. BHI broth is often used in food safety, water safety, and antibiotic sensitivity tests. Uses BHI is broadly used for culturing a variety of microorganisms, both in clinical and research settings. A number of fastidious organisms, including some bacteria, yeasts, and other fungi, grow well on BHI. It can also be used to differentiate between enterococci and group D streptococci. History The earliest version of brain heart infusion media was made in 1899 when Edward Rosenow combined dextrose broth with calf brain tissue to grow streptococci. This was modified ...
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BHI Media
BHI may refer to: * Beverly Hills Internet, former name of GeoCities, a web hosting service * BHI, station code for Birmingham International railway station, a United Kingdom railway station * Black Hebrew Israelites, African Americans who claim that they are descendants of the ancient Israelites * Black Hole Initiative, an interdisciplinary science program at Harvard University * Bristol Heart Institute, research and medical clinic in Bristol, England * British Horological Institute, the representative body of the horological industry in the United Kingdom * Brain heart infusion broth, a growth medium for growing microorganisms * Bald Head Island, North Carolina, a village located in Brunswick County, North Carolina, United States * BHI, former stock symbol of Baker Hughes, a General Electric (American) industrial service company * BHI, inventory number code assigned to all specimen or sample included in the catalogue of the Black Hills Institute of Geological Research (for example, ...
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Food Safety
Food safety (or food hygiene) is used as a scientific method/discipline describing handling, preparation, and storage of food in ways that prevent food-borne illness. The occurrence of two or more cases of a similar illness resulting from the ingestion of a common food is known as a food-borne disease outbreak. This includes a number of routines that should be followed to avoid potential health hazards. In this way, food safety often overlaps with food defense to prevent harm to consumers. The tracks within this line of thought are safety between industry and the market and then between the market and the consumer. In considering industry to market practices, food safety considerations include the origins of food including the practices relating to food labeling, food hygiene, food additives and pesticide residues, as well as policies on biotechnology and food and guidelines for the management of governmental import and export inspection and certification systems for fo ...
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Agar
Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from ogonori (''Gracilaria'') and "tengusa" (''Gelidiaceae''). As found in nature, agar is a mixture of two components, the linear polysaccharide agarose and a heterogeneous mixture of smaller molecules called agaropectin. It forms the supporting structure in the cell walls of certain species of algae and is released on boiling. These algae are known as agarophytes, belonging to the Rhodophyta (red algae) phylum. The processing of food-grade agar removes the agaropectin, and the commercial product is essentially pure agarose. Agar has been used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture media for microbiological work. Agar can be used as a laxative; an appetite suppressant; a vegan substitute for gelatin; a thickener for soups; in fruit preserves, ice cream, and other ...
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Gelatin
Gelatin or gelatine (from la, gelatus meaning "stiff" or "frozen") is a translucent, colorless, flavorless food ingredient, commonly derived from collagen taken from animal body parts. It is brittle when dry and rubbery when moist. It may also be referred to as hydrolyzed collagen, collagen hydrolysate, gelatine hydrolysate, hydrolyzed gelatine, and collagen peptides after it has undergone hydrolysis. It is commonly used as a gelling agent in food, beverages, medications, drug or vitamin capsules, photographic films, papers, and cosmetics. Substances containing gelatin or functioning in a similar way are called gelatinous substances. Gelatin is an irreversibly hydrolyzed form of collagen, wherein the hydrolysis reduces protein fibrils into smaller peptides; depending on the physical and chemical methods of denaturation, the molecular weight of the peptides falls within a broad range. Gelatin is present in gelatin desserts, most gummy candy and marshmallows, ice creams, di ...
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Disodium Phosphate
Disodium phosphate (DSP), or disodium hydrogen phosphate, or sodium phosphate dibasic, is the inorganic compound with the formula Na2HPO4. It is one of several sodium phosphates. The salt is known in anhydrous form as well as forms with 2, 7, 8, and 12 hydrates. All are water-soluble white powders; the anhydrous salt being hygroscopic. The pH of disodium hydrogen phosphate water solution is between 8.0 and 11.0, meaning it is moderately basic: :HPO42− + H2O H2PO4− + OH− Production and reactions It can be generated by neutralization of phosphoric acid with sodium hydroxide: :H3PO4 + 2 NaOH → Na2HPO4 + 2 H2O Industrially It is prepared in a two-step process by treating dicalcium phosphate with sodium bisulfate, which precipitates calcium sulfate:Klaus Schrödter, Gerhard Bettermann, Thomas Staffel, Friedrich Wahl, Thomas Klein, Thomas Hofmann "Phosphoric Acid and Phosphates" in ''Ullmann’s Encyclopedia of Industrial Chemistry'' 2008, Wiley-VCH, W ...
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Antibiotic Sensitivity
Antibiotic sensitivity testing or antibiotic susceptibility testing is the measurement of the susceptibility of bacteria to antibiotics. It is used because bacteria may have resistance to some antibiotics. Sensitivity testing results can allow a clinician to change the choice of antibiotics from empiric therapy, which is when an antibiotic is selected based on clinical suspicion about the site of an infection and common causative bacteria, to directed therapy, in which the choice of antibiotic is based on knowledge of the organism and its sensitivities. Sensitivity testing usually occurs in a medical laboratory, and uses culture methods that expose bacteria to antibiotics, or genetic methods that test to see if bacteria have genes that confer resistance. Culture methods often involve measuring the diameter of areas without bacterial growth, called zones of inhibition, around paper discs containing antibiotics on agar culture dishes that have been evenly inoculated with bacter ...
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Water Safety
Water safety refers to the procedures, precautions and policies associated with safety in, on, and around bodies of water, where there is a risk of injury or drowning. It has applications in several occupations, sports and recreational activities. Associated industries Manufacture of marine and aquatic safety equipment Associated occupations Lifeguard A lifeguard is a rescuer who supervises the safety and rescue of swimmers, surfers, and other water sports participants such as in a swimming pool, water park, beach, spa, river and lake. Lifeguards are trained in swimming and CPR/AED first aid, certified in water rescue using a variety of aids and equipment depending on requirements of their particular venue. In some areas, lifeguards are part of the emergency services system to incidents and in some communities, lifeguards may function as the primary EMS provider. Air-sea rescue Air-sea rescue (ASR or A/SR, also known as sea-air rescue ) is the coordinated search and rescu ...
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Porcine
The pig (''Sus domesticus''), often called swine, hog, or domestic pig when distinguishing from other members of the genus '' Sus'', is an omnivorous, domesticated, even-toed, hoofed mammal. It is variously considered a subspecies of ''Sus scrofa'' (the wild boar or Eurasian boar) or a distinct species. The pig's head-plus-body length ranges from , and adult pigs typically weigh between , with well-fed individuals even exceeding this range. The size and weight of hogs largely depends on their breed. Compared to other artiodactyls, a pig's head is relatively long and pointed. Most even-toed ungulates are herbivorous, but pigs are omnivores, like their wild relative. Pigs grunt and make snorting sounds. When used as livestock, pigs are farmed primarily for the production of meat, called pork. A group of pigs is called a ''passel'', a ''team'', or a ''sounder''. The animal's bones, hide, and bristles are also used in products. Pigs, especially miniature breeds, are kept as p ...
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Growth Medium
A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss ''Physcomitrella patens''. Different types of media are used for growing different types of cells. The two major types of growth media are those used for cell culture, which use specific cell types derived from plants or animals, and those used for microbiological culture, which are used for growing microorganisms such as bacteria or fungi. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellular parasites and require a growth medium containing living cells. Types The most common growth media ...
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Bovine
Bovines (subfamily Bovinae) comprise a diverse group of 10 genera of medium to large-sized ungulates, including cattle, bison, African buffalo, water buffalos, and the four-horned and spiral-horned antelopes. The evolutionary relationship between the members of the group is still debated, and their classification into loose tribes rather than formal subgroups reflects this uncertainty. General characteristics include cloven hooves and usually at least one of the sexes of a species having true horns. The largest extant bovine is the gaur. In many countries, bovid milk and meat is used as food by humans. Cattle are kept as livestock almost everywhere except in parts of India and Nepal, where they are considered sacred by most Hindus. Bovids are used as draft animals and as riding animals. Small breeds of domestic bovid, such as the Miniature Zebu, are kept as pets. Bovid leather is durable and flexible and is used to produce a wide range of goods including clothing and bags. ...
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Meningococci
''Neisseria meningitidis'', often referred to as meningococcus, is a Gram-negative bacterium that can cause meningitis and other forms of meningococcal disease such as meningococcemia, a life-threatening sepsis. The bacterium is referred to as a coccus because it is round, and more specifically a diplococcus because of its tendency to form pairs. About 10% of adults are carriers of the bacteria in their nasopharynx. As an exclusively human pathogen, it is the main cause of bacterial meningitis in children and young adults, causing developmental impairment and death in about 10% of cases. It causes the only form of bacterial meningitis known to occur epidemically, mainly in Africa and Asia. It occurs worldwide in both epidemic and endemic form. ''N. meningitidis'' is spread through saliva and respiratory secretions during coughing, sneezing, kissing, chewing on toys and through sharing a source of fresh water. It has also been reported to be transmitted through oral sex and caus ...
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