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Beaming
BEAMing, which stands for beads, emulsion, amplification, magnetics, is a highly sensitive digital PCR method that combines emulsion PCR and flow cytometry to identify and quantify specific somatic mutations present in DNA. Process BEAMing begins with the isolation of DNA from a patient’s blood or plasma sample. Target regions of the purified DNA undergo a pre-amplification step with conventional PCR utilizing primers of known sequences to amplify the genetic regions of interest. The amplified DNA templates are then introduced to primers that are covalently bound to magnetic beads via streptavidin- biotin interactions and are compartmentalized into aqueous microdroplets of a water-in-oil emulsion. The aqueous phase is emulsified with the oil, creating millions of individual water droplets having a diameter of 3-10 microns. Within each droplet, a separate PCR reaction is performed. Due to the small size, each water droplet contains on average a single DNA molecule and a magneti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Digital PCR
Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnology, biotechnological refinement of conventional polymerase chain reaction methods that can be used to directly quantify and clonally amplify nucleic acids strands including DNA, cDNA, or RNA. The key difference between dPCR and traditional PCR lies in the method of measuring nucleic acids amounts, with the former being a more precise method than PCR, though also more prone to error in the hands of inexperienced users. A "digital" measurement quantitatively and discretely measures a certain variable, whereas an “analog” measurement extrapolates certain measurements based on measured patterns. PCR carries out one reaction per single sample. dPCR also carries out a single reaction within a sample, however the sample is separated into a large number of partitions and the reaction is carried out in each partition individually. This separation allows a more reliable collection and sensitive mea ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Liquid Biopsy
A liquid biopsy, also known as fluid biopsy or fluid phase biopsy, is the sampling and analysis of non-solid biological tissue, primarily blood. Like traditional biopsy, this type of technique is mainly used as a diagnostic and monitoring tool for diseases such as cancer, with the added benefit of being largely non-invasive. Liquid biopsies may also be used to validate the efficiency of a cancer treatment drug by taking multiple samples in the span of a few weeks. The technology may also prove beneficial for patients after treatment to monitor relapse. The clinical implementation of liquid biopsies is not yet widespread but is becoming standard of care in some areas. Types There are several types of liquid biopsy methods; method selection depends on the condition that is being studied. A wide variety of biomarkers may be studied to detect or monitor other diseases. For example, isolation of protoporphyrin IX from blood samples can be used as a diagnostic tool for atheroscleros ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nature Medicine
''Nature Medicine'' is a monthly Peer review, peer-reviewed medical journal published by Nature Portfolio covering all aspects of medicine. It was established in 1995. The journal seeks to publish research papers that "demonstrate novel insight into disease processes, with direct evidence of the physiological relevance of the results". As with other ''Nature'' journals, there is no external editorial board, with editorial decisions being made by an in-house team, although peer review by external expert referees forms a part of the review process. The editor-in-chief is João Monteiro (editor), João Monteiro. According to the ''Journal Citation Reports'', the journal has a 2021 impact factor of 87.241, ranking it 1st out of 296 journals in the category "Biochemistry & Molecular Biology". References External links * {{Portal bar, Medicine Publications established in 1995 Nature Research academic journals General medical journals Monthly journals English-language journa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nature Methods
''Nature Methods'' is a monthly peer-reviewed scientific journal covering new scientific techniques. It was established in 2004 and is published by Springer Nature under the Nature Portfolio. Like other ''Nature'' journals, there is no external editorial board and editorial decisions are made by an in-house team, although peer review by external experts forms a part of the review process. Every year, the journal highlights a field, approach, or technique that has enabled recent major advances in life sciences research as the "Method of the Year". According to the ''Journal Citation Reports'', the journal had a 2021 impact factor The impact factor (IF) or journal impact factor (JIF) of an academic journal is a scientometric index calculated by Clarivate that reflects the yearly mean number of citations of articles published in the last two years in a given journal, as i ... of 47.99, ranking it first in the category "Biochemical Research Methods". References External link ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Colorectal Cancer
Colorectal cancer (CRC), also known as bowel cancer, colon cancer, or rectal cancer, is the development of cancer from the colon or rectum (parts of the large intestine). Signs and symptoms may include blood in the stool, a change in bowel movements, weight loss, and fatigue. Most colorectal cancers are due to old age and lifestyle factors, with only a small number of cases due to underlying genetic disorders. Risk factors include diet, obesity, smoking, and lack of physical activity. Dietary factors that increase the risk include red meat, processed meat, and alcohol. Another risk factor is inflammatory bowel disease, which includes Crohn's disease and ulcerative colitis. Some of the inherited genetic disorders that can cause colorectal cancer include familial adenomatous polyposis and hereditary non-polyposis colon cancer; however, these represent less than 5% of cases. It typically starts as a benign tumor, often in the form of a polyp, which over time becomes cancerous. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
