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Zero-mode Waveguide
zero-mode waveguide is an optical waveguide that guides light energy into a volume that is small in all dimensions compared to the wavelength of the light. Zero-mode waveguides have been developed for rapid parallel sensing of zeptolitre sample volumes, as applied to gene sequencing, by Pacific Biosciences (previously named Nanofluidics, Inc.) A waveguide operated at frequencies lower than its cutoff frequency (wavelengths longer than its cutoff wavelength) and used as a precision attenuator is also known as a "waveguide below-cutoff attenuator." The zero-mode waveguide is made possible by creating circular or rectangular nanoapertures using focused ion beam on an aluminium layer. The zero-mode waveguide can also enhance fluorescence signals due to surface plasmon generated at metal-dielectric interfaces. Due to surface plasmon generation field is localized and enhanced as well as it changes the LDOS inside the cavity which leads to increase in Purcell Factor of analyte molec ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Optical Waveguide
An optical waveguide is a physical structure that guides electromagnetic waves in the optical spectrum. Common types of optical waveguides include optical fiber waveguides, transparent dielectric waveguides made of plastic and glass, liquid light guides, and liquid waveguides. Optical waveguides are used as components in integrated optical circuits or as the transmission medium in local and long-haul optical communication systems. Optical waveguides can be classified according to their geometry (planar, strip, or fiber waveguides), mode structure (single-mode, multi-mode), refractive index distribution (step or gradient index), and material (glass, polymer, semiconductor). Total internal reflection The basic principles behind optical waveguides can be described using the concepts of geometrical or ray optics, as illustrated in the diagram. Light passing into a medium with higher refractive index bends toward the normal by the process of refraction (Figure a.). Take, for examp ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Wavelength
In physics, the wavelength is the spatial period of a periodic wave—the distance over which the wave's shape repeats. It is the distance between consecutive corresponding points of the same phase on the wave, such as two adjacent crests, troughs, or zero crossings, and is a characteristic of both traveling waves and standing waves, as well as other spatial wave patterns. The inverse of the wavelength is called the spatial frequency. Wavelength is commonly designated by the Greek letter ''lambda'' (λ). The term ''wavelength'' is also sometimes applied to modulated waves, and to the sinusoidal envelopes of modulated waves or waves formed by interference of several sinusoids. Assuming a sinusoidal wave moving at a fixed wave speed, wavelength is inversely proportional to frequency of the wave: waves with higher frequencies have shorter wavelengths, and lower frequencies have longer wavelengths. Wavelength depends on the medium (for example, vacuum, air, or water) that a wav ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Zeptolitre
The litre (international spelling) or liter (American English spelling) (SI symbols L and l, other symbol used: ℓ) is a metric unit of volume. It is equal to 1 cubic decimetre (dm3), 1000 cubic centimetres (cm3) or 0.001 cubic metre (m3). A cubic decimetre (or litre) occupies a volume of (see figure) and is thus equal to one-thousandth of a cubic metre. The original French metric system used the litre as a base unit. The word ''litre'' is derived from an older French unit, the '' litron'', whose name came from Byzantine Greek—where it was a unit of weight, not volume—via Late Medieval Latin, and which equalled approximately 0.831 litres. The litre was also used in several subsequent versions of the metric system and is accepted for use with the SI,Bureau International des Poids et Me ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
DNA Sequencing
DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Knowledge of DNA sequences has become indispensable for basic biological research, DNA Genographic Projects and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. Comparing healthy and mutated DNA sequences can diagnose different diseases including various cancers, characterize antibody repertoire, and can be used to guide patient treatment. Having a quick way to sequence DNA allows for faster and more individualized medical care to be administered, and for more organisms to be identified and cataloged. The rapid speed of sequencing attained with modern D ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pacific Biosciences
Pacific Biosciences of California, Inc. (aka PacBio) is an American biotechnology company founded in 2004 that develops and manufactures systems for gene sequencing and some novel real time biological observation. PacBio describes its platform as single-molecule real-time sequencing (SMRT), based on the properties of zero-mode waveguides. History The company was founded based on research done at Cornell University that combined semiconductor processing and photonics with biotechnology research. Three graduate students in the lab of Professors Watt W. Webb — Jonas Korlach — and Harold Craighead — Steve Turner and Mathieu Foquet — became the first employees. It began under the name Nanofluidics, Inc. The company raised nearly in six rounds of primarily venture capital financing, making it one of the most capitalized startups in 2010 leading up to their public offering in October of that year. Key investors included Mohr Davidow Ventures, Kleiner, P ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cutoff Frequency
In physics and electrical engineering, a cutoff frequency, corner frequency, or break frequency is a boundary in a system's frequency response at which energy flowing through the system begins to be reduced ( attenuated or reflected) rather than passing through. Typically in electronic systems such as filters and communication channels, cutoff frequency applies to an edge in a lowpass, highpass, bandpass, or band-stop characteristic – a frequency characterizing a boundary between a passband and a stopband. It is sometimes taken to be the point in the filter response where a transition band and passband meet, for example, as defined by a half-power point (a frequency for which the output of the circuit is −3 Decibel, dB of the nominal passband value). Alternatively, a stopband corner frequency may be specified as a point where a transition band and a stopband meet: a frequency for which the attenuation is larger than the required stopband attenuation, which for example ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cutoff Wavelength
In physics and electrical engineering, a cutoff frequency, corner frequency, or break frequency is a boundary in a system's frequency response at which energy flowing through the system begins to be reduced ( attenuated or reflected) rather than passing through. Typically in electronic systems such as filters and communication channels, cutoff frequency applies to an edge in a lowpass, highpass, bandpass, or band-stop characteristic – a frequency characterizing a boundary between a passband and a stopband. It is sometimes taken to be the point in the filter response where a transition band and passband meet, for example, as defined by a half-power point (a frequency for which the output of the circuit is −3 dB of the nominal passband value). Alternatively, a stopband corner frequency may be specified as a point where a transition band and a stopband meet: a frequency for which the attenuation is larger than the required stopband attenuation, which for example may be 3 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Attenuator (electronics)
An attenuator is an electronic device that reduces the power of a signal without appreciably distorting its waveform. An attenuator is effectively the opposite of an amplifier, though the two work by different methods. While an amplifier provides gain, an attenuator provides loss, or gain less than 1. Construction and usage Attenuators are usually passive devices made from simple voltage divider networks. Switching between different resistances forms adjustable stepped attenuators and continuously adjustable ones using potentiometers. For higher frequencies precisely matched low VSWR resistance networks are used. Fixed attenuators in circuits are used to lower voltage, dissipate power, and to improve impedance matching. In measuring signals, attenuator pads or adapters are used to lower the amplitude of the signal a known amount to enable measurements, or to protect the measuring device from signal levels that might damage it. Attenuators are also used to 'match' impedance b ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Focused Ion Beam
Focused ion beam, also known as FIB, is a technique used particularly in the semiconductor industry, materials science and increasingly in the biological field for site-specific analysis, deposition, and ablation of materials. A FIB setup is a scientific instrument that resembles a scanning electron microscope (SEM). However, while the SEM uses a focused beam of electrons to image the sample in the chamber, a FIB setup uses a focused beam of ions instead. FIB can also be incorporated in a system with both electron and ion beam columns, allowing the same feature to be investigated using either of the beams. FIB should not be confused with using a beam of focused ions for direct write lithography (such as in proton beam writing). These are generally quite different systems where the material is modified by other mechanisms. Ion beam source Most widespread instruments are using liquid metal ion sources (LMIS), especially gallium ion sources. Ion sources based on elemental gold an ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Single Molecule Real-time Sequencing
Single may refer to: Arts, entertainment, and media * Single (music), a song release Songs * "Single" (Natasha Bedingfield song), 2004 * "Single" (New Kids on the Block and Ne-Yo song), 2008 * "Single" (William Wei song), 2016 * "Single", by Meghan Trainor from the album '' Only 17'' Sports * Single (baseball), the most common type of base hit * Single (cricket), point in cricket * Single (football), Canadian football point * Single-speed bicycle Transportation * Single-cylinder engine, an internal combustion engine design with one cylinder, or a motorcycle using such engine * Single (locomotive), a steam locomotive with a single pair of driving wheels * As a verb: to convert a double-track railway to a single-track railway Other uses * Single (mathematics) (1-tuple), a list or sequence with only one element * Single person, a person who is not in a committed relationship * Single precision, a computer numbering format that occupies one storage location in computer memory at ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biophysics
Biophysics is an interdisciplinary science that applies approaches and methods traditionally used in physics to study biological phenomena. Biophysics covers all scales of biological organization, from molecular to organismic and populations. Biophysical research shares significant overlap with biochemistry, molecular biology, physical chemistry, physiology, nanotechnology, bioengineering, computational biology, biomechanics, developmental biology and systems biology. The term ''biophysics'' was originally introduced by Karl Pearson in 1892.Roland Glaser. Biophysics: An Introduction'. Springer; 23 April 2012. . The term ''biophysics'' is also regularly used in academia to indicate the study of the physical quantities (e.g. electric current, temperature, stress, entropy) in biological systems. Other biological sciences also perform research on the biophysical properties of living organisms including molecular biology, cell biology, chemical biology, and biochemistry. Overview ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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