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Yeast Expression Platforms
A yeast expression platform is a strain of yeast used to produce large amounts of proteins, sugars or other compounds for research or industrial uses. While yeast are often more resource-intensive to maintain than bacteria, certain products can only be produced by eukaryotic cells like yeast, necessitating use of a yeast expression platform. Yeasts differ in productivity and with respect to their capabilities to secrete, process and modify proteins. As such, different types of yeast (i.e. different expression platforms) are better suited for different research and industrial applications. Products Since the onset of genetic engineering, a number of microorganisms have been developed for the production of biological products. These products are used in medicine and industry to create pharmaceuticals like hepatitis B vaccines or insulin. Common platforms for the development of medicine and other products include the bacterium ''E. coli'', and several yeasts and mammalian ce ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Yeast
Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized. They are estimated to constitute 1% of all described fungal species. Yeasts are unicellular organisms that evolved from multicellular ancestors, with some species having the ability to develop multicellular characteristics by forming strings of connected budding cells known as pseudohyphae or false hyphae. Yeast sizes vary greatly, depending on species and environment, typically measuring 3–4 µm in diameter, although some yeasts can grow to 40 µm in size. Most yeasts reproduce asexually by mitosis, and many do so by the asymmetric division process known as budding. With their single-celled growth habit, yeasts can be contrasted with molds, which grow hyphae. Fungal species that can take both forms (depending on temperature or other conditions) are ca ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Budding
Budding or blastogenesis is a type of asexual reproduction in which a new organism develops from an outgrowth or bud due to cell division at one particular site. For example, the small bulb-like projection coming out from the yeast cell is known as a bud. Since the reproduction is asexual, the newly created organism is a clone and excepting mutations is genetically identical to the parent organism. Organisms such as hydra use regenerative cells for reproduction in the process of budding. In hydra, a bud develops as an outgrowth due to repeated cell division at one specific site. These buds develop into tiny individuals and, when fully mature, detach from the parent body and become new independent individuals. Internal budding or endodyogeny is a process of asexual reproduction, favored by parasites such as ''Toxoplasma gondii''. It involves an unusual process in which two daughter cells are produced inside a mother cell, which is then consumed by the offspring prior to their s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chymosin
Chymosin or rennin is a protease found in rennet. It is an aspartic endopeptidase belonging to MEROPS A1 family. It is produced by newborn ruminant animals in the lining of the abomasum to curdle the milk they ingest, allowing a longer residence in the bowels and better absorption. It is widely used in the production of cheese. Bovine chymosin is now produced recombinantly in , '' Aspergillus niger var awamori'', and as alternative resource. Occurrence The chymosin is found in a wide range of tetrapods, although it is best known to be produced by ruminant animals in the lining of the abomasum. Chymosin is produced by gastric chief cells in newborn mammals to curdle the milk they ingest, allowing a longer residence in the bowels and better absorption. Non-ruminant species that produce chymosin include pigs, cats, seals,Staff, Online Mendelian Inheritance in Man (OMIM) Database. Last updated February 21, 199Chymosin pseudogene; CYMP prochymosin, included, in the OMIM/ref> a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Lactose
Lactose is a disaccharide sugar synthesized by galactose and glucose subunits and has the molecular formula C12H22O11. Lactose makes up around 2–8% of milk (by mass). The name comes from ' (gen. '), the Latin word for milk, plus the suffix '' -ose'' used to name sugars. The compound is a white, water-soluble, non-hygroscopic solid with a mildly sweet taste. It is used in the food industry. Structure and reactions Lactose is a disaccharide derived from the condensation of galactose and glucose, which form a β-1→4 glycosidic linkage. Its systematic name is β-D-galactopyranosyl-(1→4)-D-glucose. The glucose can be in either the α-pyranose form or the β-pyranose form, whereas the galactose can only have the β-pyranose form: hence α-lactose and β-lactose refer to the anomeric form of the glucopyranose ring alone. Detection reactions for lactose are the Woehlk- and Fearon's test. Both can be easily used in school experiments to visualise the different lactose content o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Kefir
Kefir ( ; also spelled as kephir or kefier; ; ; ) is a fermented milk drink similar to a thin yogurt or ayran that is made from kefir grains, a specific type of mesophilic symbiotic culture. The drink originated in the North Caucasus, in particular the Elbrus region along the upper mountainous sections of Circassia, Karachay and Balkaria, from where it came to Russia, and from there it spread to Europe and the United States, where it is prepared by inoculating the milk of cows, goats, or sheep with kefir grains. Kefir is a breakfast, lunch, and dinner drink popular across Russia, Belarus, Estonia, Hungary, Latvia, Lithuania, Poland, Romania, and Ukraine - where it is known as an affordable health drink. It is also known in Norway, Sweden, and Finland, where buttermilk-type fermented dairy drinks are common. Kefir is common particularly among Russian and Estonian minorities. In South Slavic countries, kefir is consumed at any time of the day, especially with zelnik/zelja ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Kluyveromyces Lactis
''Kluyveromyces lactis'' is a ''Kluyveromyces'' yeast commonly used for genetic studies and industrial applications. Its name comes from the ability to assimilate lactose and convert it into lactic acid. ''Kluyveromyces lactis'' (formerly ''Saccharomyces lactis'') is a yeast which has the ability to assimilate lactose and convert it into lactic acid. ''K. lactis'' and other organisms i.e., ''Aspergillus niger'' var awamori and ''Escherichia coli'' K-12 are grown in fermenters to produce chymosin ( rennet) on a commercial scale; this rennet, which replaces the conventional form obtained from slaughtered animals, is now widely used in cheese production. Yeasts and fungi are ideal organisms for comparative genomic studies in eukaryotes because of their small and compact genomes and because they include a number of species such as ''Neurospora crassa'', ''Saccharomyces cerevisiae'' and ''Schizosaccharomyces pombe'', that have been, and continue to be, used extensively in genetic st ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ogataea Polymorpha
''Ogataea polymorpha'' is a methylotrophic yeast with unusual characteristics. It is used as a protein factory for pharmaceuticals. ''Ogataea polymorpha'' belongs to a limited number of methylotrophic yeast species – yeasts that can grow on methanol. The range of methylotrophic yeasts includes ''Candida boidinii'', ''Pichia methanolica'', '' Pichia pastoris'' and ''Ogataea polymorpha''. ''O. polymorpha'' is taxonomically a species of the family Saccharomycetaceae. Strains Three ''O. polymorpha'' strains, identified in the 1950s, are known. They have unclear relationships and are of independent origins. They are found in soil samples, the gut of insects or in spoiled concentrated orange juice. They exhibit different features and are used in basic research and to recombinant protein production: *strain CBS4732 (CCY38-22-2; ATCC34438, NRRL-Y-5445) *strain DL-1 (NRRL-Y-7560; ATCC26012) *strain NCYC495 (CBS1976; ATAA14754, NRLL-Y-1798) Strains CBS4732 and NCYY495 can be mated ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Xylose Reductase
Xylose ( grc, ξύλον, , "wood") is a sugar first isolated from wood, and named for it. Xylose is classified as a monosaccharide of the aldopentose type, which means that it contains five carbon atoms and includes an aldehyde functional group. It is derived from hemicellulose, one of the main constituents of biomass. Like most sugars, it can adopt several structures depending on conditions. With its free aldehyde group, it is a reducing sugar. Structure The acyclic form of xylose has chemical formula . The cyclic hemiacetal isomers are more prevalent in solution and are of two types: the pyranoses, which feature six-membered rings, and the furanoses, which feature five-membered rings (with a pendant group). Each of these rings is subject to further isomerism, depending on the relative orientation of the anomeric hydroxy group. The dextrorotary form, -xylose, is the one that usually occurs endogenously in living things. A levorotary form, -xylose, can be s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gram
The gram (originally gramme; SI unit symbol g) is a Physical unit, unit of mass in the International System of Units (SI) equal to one one thousandth of a kilogram. Originally defined as of 1795 as "the absolute weight of a volume of pure water equal to Cube (algebra), the cube of the hundredth part of a metre [1 Cubic centimetre, cm3], and at Melting point of water, the temperature of Melting point, melting ice", the defining temperature (~0 °C) was later changed to 4 °C, the temperature of maximum density of water. However, by the late 19th century, there was an effort to make the Base unit (measurement), base unit the kilogram and the gram a derived unit. In 1960, the new International System of Units defined a ''gram'' as one one-thousandth of a kilogram (i.e., one gram is Scientific notation, 1×10−3 kg). The kilogram, 2019 redefinition of the SI base units, as of 2019, is defined by the International Bureau of Weights and Measures from the fixed numeric ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pichia Pastoris
''Pichia pastoris'' is a species of methylotrophic yeast. It was found in the 1960s, with its feature of using methanol as a source of carbon and energy. After years of study, ''P. pastoris'' was widely used in biochemical research and biotech industries. With strong potential for being an expression system for protein production, as well as being a model organism for genetic study, ''P. pastoris'' has become important for biological research and biotech applications. In the last decade, some reports reassigned ''P. pastoris'' to the genus ''Komagataella'' with phylogenetic analysis, by genome sequencing of ''P. pastoris''. The genus was split into ''K. phaffii'', ''K. pastoris'', and ''K. pseudopastoris''. ''P. pastoris'' in nature Natural habitat In nature, ''P. pastoris'' is found on trees, such as chestnut trees. They are heterotrophs and they can use several carbon sources for living, like glucose, glycerol and methanol. However, they cannot use lactose. Reproduction ' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Hansenula Polymorpha
''Ogataea polymorpha'' is a methylotrophic yeast with unusual characteristics. It is used as a protein factory for pharmaceuticals. ''Ogataea polymorpha'' belongs to a limited number of methylotrophic yeast species – yeasts that can grow on methanol. The range of methylotrophic yeasts includes ''Candida boidinii'', ''Pichia methanolica'', ''Pichia pastoris'' and ''Ogataea polymorpha''. ''O. polymorpha'' is taxonomically a species of the family Saccharomycetaceae. Strains Three ''O. polymorpha'' strains, identified in the 1950s, are known. They have unclear relationships and are of independent origins. They are found in soil samples, the gut of insects or in spoiled concentrated orange juice. They exhibit different features and are used in basic research and to recombinant protein production: *strain CBS4732 (CCY38-22-2; ATCC34438, NRRL-Y-5445) *strain DL-1 (NRRL-Y-7560; ATCC26012) *strain NCYC495 (CBS1976; ATAA14754, NRLL-Y-1798) Strains CBS4732 and NCYY495 can be mated wher ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Methylotrophic
Methylotrophs are a diverse group of microorganisms that can use reduced one-carbon compounds, such as methanol or methane, as the carbon source for their growth; and multi-carbon compounds that contain no carbon-carbon bonds, such as dimethyl ether and dimethylamine. This group of microorganisms also includes those capable of assimilating reduced one-carbon compounds by way of carbon dioxide using the ribulose bisphosphate pathway. These organisms should not be confused with methanogens which on the contrary produce methane as a by-product from various one-carbon compounds such as carbon dioxide. Some methylotrophs can degrade the greenhouse gas methane, and in this case they are called methanotrophs. The abundance, purity, and low price of methanol compared to commonly used sugars make methylotrophs competent organisms for production of amino acids, vitamins, recombinant proteins, single-cell proteins, co-enzymes and cytochromes. Metabolism The key intermediate in methylotr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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