Wide-field Multiphoton Microscopy
Wide-field multiphoton microscopy refers to an Nonlinear optics, optical non-linear imaging technique tailored for ultrafast imaging in which a large area of the object is illuminated and imaged without the need for scanning. High intensities are required to induce non-linear optical processes such as Two-photon excitation microscopy, two-photon fluorescence or Second-harmonic generation, second harmonic generation. In Multiphoton fluorescence microscope, scanning multiphoton microscopes the high intensities are achieved by tightly focusing the light, and the image is obtained by beam scanning. In wide-field multiphoton microscopy the high intensities are best achieved using an Optical amplifier, optically amplified pulsed laser source to attain a large field of view (~100 μm). The image in this case is obtained as a single frame with a CCD without the need of scanning, making the technique particularly useful to visualize dynamic processes simultaneously across the object of i ...
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