Watermelon Mosaic Virus
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Watermelon Mosaic Virus
''Watermelon mosaic virus'' (WMV) also known as Marrow mosaic virus (Raychaudhuri and Varma, 1975; Varma, 1988), Melon mosaic virus (Iwaki et al., 1984; Komuro, 1962), and until recently Watermelon mosaic virus type 2 (WMV-2), is a plant pathogenic virus that causes viral infection (sometimes referred to as watermelon Mosaic disease) in many different plants. The virus itself is referred to as Watermelon Mosaic Virus II or WMV-2 and is an isolate of the U.S. WMV-2 is a ssRNA positive strand virus that is part of the Potyviridae or Potyvirus clade. Like all RNA viruses, it contains a protein capsid which protects the inner viral RNA. First described on squash in Florida, WMV arose from a unique recombination of genetic material contributed by ''Soybean mosaic virus (SMV)'' and ''Bean common mosaic virus (BCMV)'' along with ''Peanut Stripe virus (PSV)''. Biology WMV is a potyvirus whose particles are 760 nm long flexuous rods which has a single strand of RNA. This particular ...
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University Of Idaho
The University of Idaho (U of I, or UIdaho) is a public land-grant research university in Moscow, Idaho. It is the state's land-grant and primary research university,, and the lead university in the Idaho Space Grant Consortium. The University of Idaho was the state's sole university for 71 years, until 1963. Its College of Law, established in 1909, was first accredited by the American Bar Association in 1925. Formed by the Idaho Territory legislature on January 30, 1889, the university opened its doors in 1892 on October 3, with an initial class of 40 students. The first graduating class in 1896 contained two men and two women. It has an enrollment exceeding 12,000, with over 11,000 on the Moscow campus. The university offers 142 degree programs, from accountancy to wildlife resources, including bachelor's, master's, doctoral, and specialists' degrees, and accompanyinhonors programs Certificates of completion are offered in 30 areas of study. At 25% and 53%, its 4 and 6 y ...
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Chenopodium Amaranticolor
''Chenopodium giganteum'', also known as tree spinach, is an annual, upright many-branched shrub with a stem diameter of up to 5 cm at the base, that can grow to a height of up to 3 m.Zhu, Gelin & Mosyakin, Sergei & E. Clemants, Steven. (2003). Chenopodiaceae (Flora of China). in ''Flora of China'', Volume 5, Chapter: Chenopodiaceae, Beijing: Science Press & St. Louis: Missouri Botanical Garden Press, Wu Zhengyi, Peter H. Raven, pp. 351–414 Description The younger leaves of ''Chenopodium giganteum'' are hairy with a magenta colour and the older become green. The rhombic to ovate formed lamina can have a surface of up to 20 x 16 cm. The inflorescence consists of terminal panicles with hermaphrodite flowers, which are wind pollinated. The flowers contain 5 perianth leaves and 5 stamens. The flowering period begins in August. The seeds have a diameter of 1.5 mm. The number of chromosomes is n=54. Habitat and distribution ''Chenopodium giganteum'' belongs to the sam ...
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Viral Plant Pathogens And Diseases
Viral means "relating to viruses" (small infectious agents). Viral may also refer to: Viral behavior, or virality Memetic behavior likened that of a virus, for example: * Viral marketing, the use of existing social networks to spread a marketing message * Viral phenomenon, relating to contagion theory or the "virality" of network culture, such as a meme * Viral video, a video that quickly attains a high popularity Titled works * ''Viral'' (2016 American film), a 2016 American science fiction horror drama * ''Viral'' (2016 Hindi film), an Indian Bollywood film based on social media * ''Viral'' (web series), a 2014 Brazilian comedy web series * '' V/H/S: Viral'', an American anthology horror film * '' Viral: The Search for the Origin of COVID-19'', a book by Alina Chand and Matt Ridley See also * ''Virals'', a novel series by Kathy Reichs * Virulence Virulence is a pathogen's or microorganism's ability to cause damage to a host. In most, especially in animal system ...
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Plant Virus
Plant viruses are viruses that affect plants. Like all other viruses, plant viruses are obligate intracellular parasites that do not have the molecular machinery to replicate without a host. Plant viruses can be pathogenic to higher plants. Most plant viruses are rod-shaped, with protein discs forming a tube surrounding the viral genome; isometric particles are another common structure. They rarely have an envelope. The great majority have an RNA genome, which is usually small and single stranded (ss), but some viruses have double-stranded (ds) RNA, ssDNA or dsDNA genomes. Although plant viruses are not as well understood as their animal counterparts, one plant virus has become very recognizable: ''tobacco mosaic virus'' (TMV), the first virus to be discovered. This and other viruses cause an estimated US$60 billion loss in crop yields worldwide each year. Plant viruses are grouped into 73 genera and 49 families. However, these figures relate only to cultivated plants, which r ...
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RT-PCR
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR). Combined RT-PCR and qPCR are routinely used for analysis of gene expression and quantification of viral RNA in research and clinical settings. The close association between RT-PCR and qPCR has led to metonymic use of the term qPCR to mean RT-PCR. Such use may be confusing, as RT-PCR can be used without qPCR, for example to enable molecular cloning, sequencing or simple detection of RNA. Conversely, qPCR may be used without RT-PCR, for example to quantify the copy number of a specific piece of DNA. Nomenclature The combine ...
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ELISA
The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries. In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody is applied over the surface so it can bind the antigen. This antibody is linked to an enzyme and then any unbound antibodies are removed. In the final step, a substance containing the enzyme's substrate is added. If there was binding, the subsequent reaction produces a detectable signal, most commonly a color change. Performing an ELISA involves at least ...
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Aphis Craccivora
''Aphis craccivora'', variously known as the cowpea aphid, groundnut aphid or black legume aphid, is a true bug in the family ''Aphididae''. Originally of probable Palearctic origin, it is now an invasive species of cosmopolitan distribution. Description ''Aphis craccivora'' is a small species of aphid. The female has a glossy black or dark brown body with a prominent cauda (tail-like protrusion), and legs in some shade of brown or yellow. The antennae have six segments and these and the limb segments, cauda and cornicles are pale proximally (close to the body) and dark distally (further from the body). The adults do not have wax on their dorsal surface but the nymphs are lightly dusted with wax. Winged females are up to long and have cross-barring on the abdomen. Wingless females are a little smaller. Distribution ''Aphis craccivora'' is believed to be Palearctic in origin but has expanded its range so that now it is near cosmopolitan. In recent years its range has extended fu ...
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Aphis Gossypii
''Aphis gossypii'' is a tiny insect, an aphid ("greenfly") in the superfamily Aphidoidea in the order Hemiptera. It is a true bug and sucks sap from plants. It is a widely distributed pest of a variety of agricultural crops in the families Cucurbitaceae, Rutaceae and Malvaceae. Common names include cotton aphid, melon aphid and melon and cotton aphid. Distribution It is not known where this species originated, but it is now found in tropical and temperate regions throughout the world except extreme northern areas. It is common in North and South America, Central Asia, Africa, Australia, Brazil, East Indies, Mexico and Hawaii and in most of Europe. It is cosmopolitan in habitat. It thrives outdoors in southern Europe but survives only under glass in northern Europe. In the former Soviet Union it is found up to 54°N. Morphology The wingless female cotton aphid has an ovoid body about two millimetres long in varying shades of green. The legs are yellow, as are the antennae whic ...
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Aphid
Aphids are small sap-sucking insects and members of the superfamily Aphidoidea. Common names include greenfly and blackfly, although individuals within a species can vary widely in color. The group includes the fluffy white woolly aphids. A typical life cycle involves flightless females giving live birth to female nymphs—who may also be already pregnant, an adaptation scientists call telescoping generations—without the involvement of males. Maturing rapidly, females breed profusely so that the number of these insects multiplies quickly. Winged females may develop later in the season, allowing the insects to colonize new plants. In temperate regions, a phase of sexual reproduction occurs in the autumn, with the insects often overwintering as eggs. The life cycle of some species involves an alternation between two species of host plants, for example between an annual crop and a woody plant. Some species feed on only one type of plant, while others are generalists, coloni ...
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Nicotiana Benthamiana
''Nicotiana benthamiana'', colloquially known as benth or benthi, is a species of ''Nicotiana'' indigenous to Australia. It is a close relative of tobacco. A synonym for this species is ''Nicotiana suaveolens'' var. ''cordifolia'', a description given by George Bentham in ''Flora Australiensis'' in 1868. This was transferred to ''Nicotiana benthamiana'' by Karel Domin in ''Bibliotheca Botanica'' (1929), honoring the original author in the specific epithet. History The plant was used by people of Australia as a stimulant, containing nicotine and other alkaloids, before the introduction of commercial tobacco ('' N. tabacum'' and '' N. rustica''). Indigenous names for it include ''tjuntiwari'' and ''muntju''. It was first collected on the north coast of Australia by Benjamin Bynoe on a voyage of in 1837. Description The herbaceous plant is found amongst rocks on hills and cliffs throughout the northern regions of Australia. Variable in height and habit, the species may be er ...
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Pathogenic Virus
A viral disease (or viral infection) occurs when an organism's body is invaded by pathogenic viruses, and infection, infectious virus particles (virions) attach to and enter susceptible cells. Structural Characteristics Basic structural characteristics, such as genome type, virion shape and replication site, generally share the same features among virus species within the same family. * Double-stranded DNA families: three are non-enveloped (''Adenoviridae'', ''Papillomaviridae'' and ''Polyomaviridae'') and two are enveloped (''Herpesviridae'' and ''Poxviridae''). All of the non-enveloped families have icosahedral capsids. * Partly double-stranded DNA viruses: ''Hepadnaviridae''. These viruses are enveloped. * One family of single-stranded DNA viruses infects humans: ''Parvoviridae''. These viruses are non-enveloped. * Positive single-stranded RNA families: three non-enveloped (''Astroviridae'', ''Caliciviridae'' and ''Picornaviridae'') and four enveloped (''Coronaviridae'', ''Fla ...
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Citrullus Lanatus
Watermelon (''Citrullus lanatus'') is a flowering plant species of the Cucurbitaceae family and the name of its edible fruit. A scrambling and trailing vine-like plant, it is a highly cultivated fruit worldwide, with more than 1,000 varieties. Watermelon is grown in favorable climates from tropical to temperate regions worldwide for its large edible fruit, which is a berry with a hard rind and no internal divisions, and is botanically called a ''pepo''. The sweet, juicy flesh is usually deep red to pink, with many black seeds, although seedless varieties exist. The fruit can be eaten raw or pickled, and the rind is edible after cooking. It may also be consumed as a juice or an ingredient in mixed beverages. Kordofan melons from Sudan are the closest relatives and may be progenitors of modern, cultivated watermelons. Wild watermelon seeds were found in Uan Muhuggiag, a prehistoric site in Libya that dates to approximately 3500. Watermelons were domesticated in north-east ...
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