Ussing Chamber
   HOME
*



picture info

Ussing Chamber
An Ussing chamber is an apparatus for measuring epithelial membrane properties. It can detect and quantify transport and barrier functions of living tissue. The Ussing chamber was invented by the Danish zoologist and physiologist Hans Ussing, Hans Henriksen Ussing in 1946. The technique is used to measure the short-circuit current as an indicator of net ion transport taking place across an epithelium. Ussing chambers are used to measure ion transport in native Tissue (biology), tissue, such as gut mucosa, and in a monolayer of cell (biology), cells grown on permeable supports. Function The Ussing chamber provides a system to measure the transport of ions, nutrients, and drugs across various epithelial tissues, (although can generate false-negative results for lipophilic substances). It consists of two halves separated by the epithelia (sheet of mucosa or monolayer of epithelial cells grown on permeable supports). Epithelia are polar in nature, i.e., they have an apical membra ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


picture info

Basolateral
The cell membrane (also known as the plasma membrane (PM) or cytoplasmic membrane, and historically referred to as the plasmalemma) is a biological membrane that separates and protects the interior of all cells from the outside environment (the extracellular space). The cell membrane consists of a lipid bilayer, made up of two layers of phospholipids with cholesterols (a lipid component) interspersed between them, maintaining appropriate membrane fluidity at various temperatures. The membrane also contains membrane proteins, including integral proteins that span the membrane and serve as membrane transporters, and peripheral proteins that loosely attach to the outer (peripheral) side of the cell membrane, acting as enzymes to facilitate interaction with the cell's environment. Glycolipids embedded in the outer lipid layer serve a similar purpose. The cell membrane controls the movement of substances in and out of cells and organelles, being selectively permeable to ions a ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


picture info

Square Wave
A square wave is a non-sinusoidal periodic waveform in which the amplitude alternates at a steady frequency between fixed minimum and maximum values, with the same duration at minimum and maximum. In an ideal square wave, the transitions between minimum and maximum are instantaneous. The square wave is a special case of a pulse wave which allows arbitrary durations at minimum and maximum amplitudes. The ratio of the high period to the total period of a pulse wave is called the duty cycle. A true square wave has a 50% duty cycle (equal high and low periods). Square waves are often encountered in electronics and signal processing, particularly digital electronics and digital signal processing. Its stochastic counterpart is a two-state trajectory. Origin and uses Square waves are universally encountered in digital switching circuits and are naturally generated by binary (two-level) logic devices. Square waves are typically generated by metal–oxide–semiconductor fi ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


picture info

Alternating Current
Alternating current (AC) is an electric current which periodically reverses direction and changes its magnitude continuously with time in contrast to direct current (DC) which flows only in one direction. Alternating current is the form in which electric power is delivered to businesses and residences, and it is the form of electrical energy that consumers typically use when they plug kitchen appliances, televisions, fans and electric lamps into a wall socket. A common source of DC power is a battery cell in a flashlight. The abbreviations ''AC'' and ''DC'' are often used to mean simply ''alternating'' and ''direct'', as when they modify ''current'' or ''voltage''. The usual waveform of alternating current in most electric power circuits is a sine wave, whose positive half-period corresponds with positive direction of the current and vice versa. In certain applications, like guitar amplifiers, different waveforms are used, such as triangular waves or square waves. Audio a ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


picture info

Electrodes
An electrode is an electrical conductor used to make contact with a nonmetallic part of a circuit (e.g. a semiconductor, an electrolyte, a vacuum or air). Electrodes are essential parts of batteries that can consist of a variety of materials depending on the type of battery. The electrophore, invented by Johan Wilcke, was an early version of an electrode used to study static electricity. Anode and cathode in electrochemical cells Electrodes are an essential part of any battery. The first electrochemical battery made was devised by Alessandro Volta and was aptly named the Voltaic cell. This battery consisted of a stack of copper and zinc electrodes separated by brine-soaked paper disks. Due to fluctuation in the voltage provided by the voltaic cell it wasn't very practical. The first practical battery was invented in 1839 and named the Daniell cell after John Frederic Daniell. Still making use of the zinc–copper electrode combination. Since then many more batteries have be ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


picture info

Potential Difference
Voltage, also known as electric pressure, electric tension, or (electric) potential difference, is the difference in electric potential between two points. In a static electric field, it corresponds to the work needed per unit of charge to move a test charge between the two points. In the International System of Units, the derived unit for voltage is named ''volt''. The voltage between points can be caused by the build-up of electric charge (e.g., a capacitor), and from an electromotive force (e.g., electromagnetic induction in generator, inductors, and transformers). On a macroscopic scale, a potential difference can be caused by electrochemical processes (e.g., cells and batteries), the pressure-induced piezoelectric effect, and the thermoelectric effect. A voltmeter can be used to measure the voltage between two points in a system. Often a common reference potential such as the ground of the system is used as one of the points. A voltage can represent either a source ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


Ringer Solution
Ringer's solution is a solution of several salts dissolved in water for the purpose of creating an isotonic solution relative to the body fluids of an animal. Ringer's solution typically contains sodium chloride, potassium chloride, calcium chloride and sodium bicarbonate, with the last used to balance the pH. Other additions can include chemical fuel sources for cells, including ATP and dextrose, as well as antibiotics and antifungals. Composition Ringer's solution typically contains NaCl, KCl, CaCl2 and NaHCO3, sometimes with other minerals such as MgCl2, dissolved in distilled water. The precise proportions of these vary from species to species, particularly between marine osmoconformers and osmoregulators. Uses Ringer's solution is frequently administered to human and veterinary patients for intravenous or subcutaneous hydration and to expand the vascular compartment in hypovolemia. It is also use in ''in vitro'' experiments on organs or tissues, such as ''in vitro' ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


picture info

Serosal
The serous membrane (or serosa) is a smooth tissue membrane of mesothelium lining the contents and inner walls of body cavities, which secrete serous fluid to allow lubricated sliding movements between opposing surfaces. The serous membrane that covers internal organs is called a ''visceral'' membrane; while the one that covers the cavity wall is called the ''parietal'' membrane. Between the two opposing serosal surfaces is often a potential space, mostly empty except for the small amount of serous fluid. The Latin anatomical name is '' tunica serosa''. Serous membranes line and enclose several body cavities, also known as serous cavities, where they secrete a lubricating fluid which reduces friction from movements. Serosa is entirely different from the adventitia, a connective tissue layer which binds together structures rather than reducing friction between them. The serous membrane covering the heart and lining the mediastinum is referred to as the pericardium, the s ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


picture info

Apical Membrane
The cell membrane (also known as the plasma membrane (PM) or cytoplasmic membrane, and historically referred to as the plasmalemma) is a biological membrane that separates and protects the interior of all cells from the outside environment (the extracellular space). The cell membrane consists of a lipid bilayer, made up of two layers of phospholipids with cholesterols (a lipid component) interspersed between them, maintaining appropriate membrane fluidity at various temperatures. The membrane also contains membrane proteins, including integral proteins that span the membrane and serve as membrane transporters, and peripheral proteins that loosely attach to the outer (peripheral) side of the cell membrane, acting as enzymes to facilitate interaction with the cell's environment. Glycolipids embedded in the outer lipid layer serve a similar purpose. The cell membrane controls the movement of substances in and out of cells and organelles, being selectively permeable to ions an ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


Basis Of Membrane Potential2
Basis may refer to: Finance and accounting *Adjusted basis, the net cost of an asset after adjusting for various tax-related items *Basis point, 0.01%, often used in the context of interest rates *Basis trading, a trading strategy consisting of the purchase of a security and the sale of a similar security **Basis of futures, the value differential between a future and the spot price **Basis (options), the value differential between a call option and a put option **Basis swap, an interest rate swap *Cost basis, in income tax law, the original cost of property adjusted for factors such as depreciation *Tax basis, cost of an asset and technology * Basis function *Basis (linear algebra) **Dual basis **Orthonormal basis **Schauder basis * Basis (universal algebra) *Basis of a matroid *Generating set of an ideal: **Gröbner basis ** Hilbert's basis theorem *Generating set of a group *Base (topology) *Change of basis *Greedoid *Normal basis *Polynomial basis *Radial basis function *S ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


picture info

Cell (biology)
The cell is the basic structural and functional unit of life forms. Every cell consists of a cytoplasm enclosed within a membrane, and contains many biomolecules such as proteins, DNA and RNA, as well as many small molecules of nutrients and metabolites.Cell Movements and the Shaping of the Vertebrate Body
in Chapter 21 of
Molecular Biology of the Cell
'' fourth edition, edited by Bruce Alberts (2002) published by Garland Science. The Alberts text discusses how the "cellular building blocks" move to shape developing embryos. It is also common to describe small molecules such as ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]  


Mucosa
A mucous membrane or mucosa is a membrane that lines various cavities in the body of an organism and covers the surface of internal organs. It consists of one or more layers of epithelial cells overlying a layer of loose connective tissue. It is mostly of endodermal origin and is continuous with the skin at body openings such as the eyes, eyelids, ears, inside the nose, inside the mouth, lips, the genital areas, the urethral opening and the anus. Some mucous membranes secrete mucus, a thick protective fluid. The function of the membrane is to stop pathogens and dirt from entering the body and to prevent bodily tissues from becoming dehydrated. Structure The mucosa is composed of one or more layers of epithelial cells that secrete mucus, and an underlying lamina propria of loose connective tissue. The type of cells and type of mucus secreted vary from organ to organ and each can differ along a given tract. Mucous membranes line the digestive, respiratory and reproductive trac ...
[...More Info...]      
[...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]