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Traut's Reagent
2-Iminothiolane is a cyclic wikt:thioimidate, thioimidate compound also known as Traut's reagent. It is a thiolating reagent that reacts with primary amine groups, such as those of amino acids, to form sulfhydryl groups. Application 2-Iminothiolane reacts with primary amines efficiently at pH 7 to 9, creating amidine compounds with a sulfhydryl group. Thus it allows for crosslinking or labeling of molecules such as proteins through use of disulfide or thioether conjugation. It was first used to thiolate a subunit of ribosome in Escherichia coli, ''E. coli'' in 1973 by Robert Traut, its namesake, and his colleagues. It also reacts with Aliphatic compound, aliphatic and phenolic hydroxyl groups at high pH, albeit at a much slower rate. References [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
