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Time Resolved Crystallography
Time resolved crystallography utilizes X-ray crystallography imaging to visualize reactions in four dimensions (x, y, z and time). This enables the studies of dynamical changes that occur in for example enzymes during their catalysis. The time dimension is incorporated by triggering the reaction of interest in the crystal prior to X-ray exposure, and then collecting the diffraction patterns at different time delays. In order to study these dynamical properties of macromolecules three criteria must be met; * The macromolecule must be biologically active in the crystalline state * It must be possible to trigger the reaction in the crystal * The intermediate of interest must be detectable, i.e. it must have a reasonable amount of concentration in the crystal (preferably over 25%). This has led to the development of several techniques that can be divided into two groups, the pump-probe method and diffusion-trapping methods. Pump-probe In the pump-probe method the reaction is first ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
X-ray Crystallography
X-ray crystallography is the experimental science determining the atomic and molecular structure of a crystal, in which the crystalline structure causes a beam of incident X-rays to diffract into many specific directions. By measuring the angles and intensities of these diffracted beams, a crystallographer can produce a three-dimensional picture of the density of electrons within the crystal. From this electron density, the mean positions of the atoms in the crystal can be determined, as well as their chemical bonds, their crystallographic disorder, and various other information. Since many materials can form crystals—such as salts, metals, minerals, semiconductors, as well as various inorganic, organic, and biological molecules—X-ray crystallography has been fundamental in the development of many scientific fields. In its first decades of use, this method determined the size of atoms, the lengths and types of chemical bonds, and the atomic-scale differences among various mat ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
X-ray
An X-ray, or, much less commonly, X-radiation, is a penetrating form of high-energy electromagnetic radiation. Most X-rays have a wavelength ranging from 10 picometers to 10 nanometers, corresponding to frequencies in the range 30 petahertz to 30 exahertz ( to ) and energies in the range 145 eV to 124 keV. X-ray wavelengths are shorter than those of UV rays and typically longer than those of gamma rays. In many languages, X-radiation is referred to as Röntgen radiation, after the German scientist Wilhelm Conrad Röntgen, who discovered it on November 8, 1895. He named it ''X-radiation'' to signify an unknown type of radiation.Novelline, Robert (1997). ''Squire's Fundamentals of Radiology''. Harvard University Press. 5th edition. . Spellings of ''X-ray(s)'' in English include the variants ''x-ray(s)'', ''xray(s)'', and ''X ray(s)''. The most familiar use of X-rays is checking for fractures (broken bones), but X-rays are also used in other ways. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Macromolecule
A macromolecule is a very large molecule important to biophysical processes, such as a protein or nucleic acid. It is composed of thousands of covalently bonded atoms. Many macromolecules are polymers of smaller molecules called monomers. The most common macromolecules in biochemistry are biopolymers (nucleic acids, proteins, and carbohydrates) and large non-polymeric molecules such as lipids, nanogels and macrocycles. Synthetic fibers and experimental materials such as carbon nanotubes are also examples of macromolecules. Definition The term ''macromolecule'' (''macro-'' + ''molecule'') was coined by Nobel laureate Hermann Staudinger in the 1920s, although his first relevant publication on this field only mentions ''high molecular compounds'' (in excess of 1,000 atoms). At that time the term ''polymer'', as introduced by Berzelius in 1832, had a different meaning from that of today: it simply was another form of isomerism for example with benzene and acetylene and had litt ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Photolysis
Photodissociation, photolysis, photodecomposition, or photofragmentation is a chemical reaction in which molecules of a chemical compound are broken down by photons. It is defined as the interaction of one or more photons with one target molecule. Photodissociation is not limited to visible light. Any photon with sufficient energy can affect the chemical bonds of a chemical compound. Since a photon energy, photon's energy is inversely proportional to its wavelength, electromagnetic radiations with the energy of visible light or higher, such as ultraviolet light, x-rays, and gamma rays can induce such reactions. Photolysis in photosynthesis Photolysis is part of the light-dependent reactions, light-dependent reaction or light phase or photochemical phase or Hill reaction of photosynthesis. The general reaction of photosynthetic photolysis can be given in terms of photons as: :\ce + 2 \text \longrightarrow \ce The chemical nature of "A" depends on the type of organism. Purple su ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Diffraction
Diffraction is defined as the interference or bending of waves around the corners of an obstacle or through an aperture into the region of geometrical shadow of the obstacle/aperture. The diffracting object or aperture effectively becomes a secondary source of the propagating wave. Italian scientist Francesco Maria Grimaldi coined the word ''diffraction'' and was the first to record accurate observations of the phenomenon in 1660. In classical physics, the diffraction phenomenon is described by the Huygens–Fresnel principle that treats each point in a propagating wavefront as a collection of individual spherical wavelets. The characteristic bending pattern is most pronounced when a wave from a coherent source (such as a laser) encounters a slit/aperture that is comparable in size to its wavelength, as shown in the inserted image. This is due to the addition, or interference, of different points on the wavefront (or, equivalently, each wavelet) that travel by paths of d ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Full Width At Half Maximum
In a distribution, full width at half maximum (FWHM) is the difference between the two values of the independent variable at which the dependent variable is equal to half of its maximum value. In other words, it is the width of a spectrum curve measured between those points on the ''y''-axis which are half the maximum amplitude. Half width at half maximum (HWHM) is half of the FWHM if the function is symmetric. The term full duration at half maximum (FDHM) is preferred when the independent variable is time. FWHM is applied to such phenomena as the duration of pulse waveforms and the spectral width of sources used for optical communications and the resolution of spectrometers. The convention of "width" meaning "half maximum" is also widely used in signal processing to define bandwidth as "width of frequency range where less than half the signal's power is attenuated", i.e., the power is at least half the maximum. In signal processing terms, this is at most −3 dB of attenuatio ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
John Keith Moffat
John 'Keith' Moffat (born 1943) is Louis Block Professor of Biochemistry and Molecular Biology and former Deputy Provost for Research at the University of Chicago. He currently heads BioCARS at Argonne National Laboratory, where he worked on the Advanced Photon Source. He is most noted for his contributions to Time resolved crystallography. He is a former Guggenheim Fellow and former Cornell University faculty member. He has a Ph.D. from King's College, Cambridge under the Nobel laureate Max Perutz at MRC-LMB and an undergraduate degree from the University of Edinburgh. He is married with an adopted son. Selected publications * * * * * See also * List of Guggenheim Fellowships awarded in 1985 * List of University of Chicago faculty * List of University of Cambridge people * List of University of Edinburgh people This is a list of notable graduates as well as non-graduate former students, academic staff, and university officials of the University of Edinburgh in Scotl ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Crystallography
Crystallography is the experimental science of determining the arrangement of atoms in crystalline solids. Crystallography is a fundamental subject in the fields of materials science and solid-state physics (condensed matter physics). The word "crystallography" is derived from the Greek word κρύσταλλος (''krystallos'') "clear ice, rock-crystal", with its meaning extending to all solids with some degree of transparency, and γράφειν (''graphein'') "to write". In July 2012, the United Nations recognised the importance of the science of crystallography by proclaiming that 2014 would be the International Year of Crystallography. denote a direction vector (in real space). * Coordinates in ''angle brackets'' or ''chevrons'' such as <100> denote a ''family'' of directions which are related by symmetry operations. In the cubic crystal system for example, would mean 00 10 01/nowiki> or the negative of any of those directions. * Miller indices in ''parentheses'' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
