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Two Photon Microscope
Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness. Unlike traditional fluorescence microscopy, where the excitation wavelength is shorter than the emission wavelength, two-photon excitation requires simultaneous excitation by two photons with longer wavelength than the emitted light. The laser is focused onto a specific location in the tissue and scanned across the sample to sequentially produce the image. Due to the non-linearity of two-photon excitation, mainly fluorophores in the micrometer-sized focus of the laser beam are excited, which results in the spatial resolution of the image. This contrasts with confocal microscopy, where the spatial resolution is produced by the interaction of excitation focus and the confined detection with a pinhole. Two-photon excitation microscopy typically uses near-infrared (NIR) excitation light whi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Wolfgang Kaiser (physicist)
Wolfgang Kaiser (17 July 1925 – 20 October 2023) was a German physicist who worked in the fields of laser and solid-state physics. Biography Wolfgang Kaiser was born in Nürnberg on 17 July 1925. He was awarded his doctorate in Erlangen in 1952, worked as scientist at Purdue University, and in 1954, he joined the US Army Signal Corps Engineering Laboratories in Fort Monmouth. From 1957 to 1964, Kaiser worked at Bell Laboratories in Murray Hill. In 1964, he became a professor for experimental physics at the Technische Universität München, where he performed research on laser physics. Kaiser died on 20 October 2023, at the age of 98. Research Kaiser was internationally recognized for his pioneering work on lasers with ultra-short pulses which find many applications in biophysical and chemical equipment. For example, he showed the molecular reactions regarding the photochemistry of bacteriorhodopsin in photosynthesis. He was involved in the development of ruby lasers and disc ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pixel
In digital imaging, a pixel (abbreviated px), pel, or picture element is the smallest addressable element in a Raster graphics, raster image, or the smallest addressable element in a dot matrix display device. In most digital display devices, pixels are the smallest element that can be manipulated through software. Each pixel is a Sampling (signal processing), sample of an original image; more samples typically provide more accurate representations of the original. The Intensity (physics), intensity of each pixel is variable. In color imaging systems, a color is typically represented by three or four component intensities such as RGB color model, red, green, and blue, or CMYK color model, cyan, magenta, yellow, and black. In some contexts (such as descriptions of camera sensors), ''pixel'' refers to a single scalar element of a multi-component representation (called a ''photosite'' in the camera sensor context, although ''wikt:sensel, sensel'' is sometimes used), while in yet ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Photomultiplier
A photomultiplier is a device that converts incident photons into an electrical signal. Kinds of photomultiplier include: * Photomultiplier tube, a vacuum tube converting incident photons into an electric signal. Photomultiplier tubes (PMTs for short) are members of the class of vacuum tubes, and more specifically vacuum phototubes, which are extremely sensitive detectors of light in the ultraviolet, visible, and near-infrared ranges of the electromagnetic spectrum. ** Magnetic photomultiplier, developed by the Soviets in the 1930s. ** Electrostatic photomultiplier, a kind of photomultiplier tube demonstrated by Jan Rajchman of RCA Laboratories in Princeton, NJ in the late 1930s which became the standard for all future commercial photomultipliers. The first mass-produced photomultiplier, the Type 931, was of this design and is still commercially produced today. * Silicon photomultiplier, a solid-state device converting incident photons into an electric signal. Silicon photomul ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Visible Spectrum
The visible spectrum is the spectral band, band of the electromagnetic spectrum that is visual perception, visible to the human eye. Electromagnetic radiation in this range of wavelengths is called ''visible light'' (or simply light). The optical spectrum is sometimes considered to be the same as the visible spectrum, but some authors define the term more broadly, to include the ultraviolet and infrared parts of the electromagnetic spectrum as well, known collectively as ''optical radiation''. A typical human eye will respond to wavelengths from about 380 to about 750 nanometers. In terms of frequency, this corresponds to a band in the vicinity of 400–790 Terahertz (unit), terahertz. These boundaries are not sharply defined and may vary per individual. Under optimal conditions, these limits of human perception can extend to 310 nm (ultraviolet) and 1100 nm (near infrared). The spectrum does not contain all the colors that the human visual system can distinguish. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ti-sapphire Laser
A titanium-sapphire laser (also known as a Ti:sapphire laser, Ti:Al2O3 laser or Ti:sapph) is a tunable laser which emits red and near-infrared light in the range from 650 to 1100 nanometers. This type of laser is mainly used in scientific research because of its tunability and its ability to generate ultrashort pulses, thanks to its broad light emission spectrum. Lasers based on Ti:sapphire were first constructed and invented in June 1982 by Peter Moulton at the MIT Lincoln Laboratory. Titanium-sapphire refers to the lasing medium, a crystal of sapphire (Al2O3) that is doped with Ti3+ ions. A Ti:sapphire laser is usually pumped with another laser with a wavelength of 514 to 532 nm, for which argon-ion lasers (514.5 nm) and frequency-doubled Nd:YAG, Nd:YLF, and Nd:YVO lasers (527–532 nm) are used. They are capable of laser operation from 670 nm to nm wavelength. Ti:sapphire lasers operate most efficiently at wavelengths near 800 nm. The crysta ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pulsed Laser
Pulsed operation of lasers refers to any laser not classified as continuous wave, so that the optical power appears in pulses of some duration at some repetition rate. This encompasses a wide range of technologies addressing a number of different motivations. Some lasers are pulsed simply because they cannot be run in continuous mode. In other cases the application requires the production of pulses having as large an energy as possible. Since the pulse energy is equal to the average power divided by the repetition rate, this goal can sometimes be satisfied by lowering the rate of pulses so that more energy can be built up in between pulses. In laser ablation for example, a small volume of material at the surface of a work piece can be evaporated if it is heated in a very short time, whereas supplying the energy gradually would allow for the heat to be absorbed into the bulk of the piece, never attaining a sufficiently high temperature at a particular point. Other applications rely ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Flux
Flux describes any effect that appears to pass or travel (whether it actually moves or not) through a surface or substance. Flux is a concept in applied mathematics and vector calculus which has many applications in physics. For transport phenomena, flux is a vector quantity, describing the magnitude and direction of the flow of a substance or property. In vector calculus flux is a scalar quantity, defined as the surface integral of the perpendicular component of a vector field over a surface. Terminology The word ''flux'' comes from Latin: ''fluxus'' means "flow", and ''fluere'' is "to flow". As '' fluxion'', this term was introduced into differential calculus by Isaac Newton. The concept of heat flux was a key contribution of Joseph Fourier, in the analysis of heat transfer phenomena. His seminal treatise ''Théorie analytique de la chaleur'' (''The Analytical Theory of Heat''), defines ''fluxion'' as a central quantity and proceeds to derive the now well-known expre ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorophore
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions). More generally they are covalently bonded to macromolecules, serving as a markers (or dyes, or tags, or reporters) for affine or bioactive reagents (antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, such as fluorescent imaging and spectroscopy. Fluorescein, via its amine-reactive isothiocyanate derivative fluorescein isothiocyanate (FITC), has been one of the most popular fluorophores ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Point Spread Function
The point spread function (PSF) describes the response of a focused optical imaging system to a point source or point object. A more general term for the PSF is the system's impulse response; the PSF is the impulse response or impulse response function (IRF) of a focused optical imaging system. The PSF in many contexts can be thought of as the shapeless blob in an image that should represent a single point object. We can consider this as a spatial impulse response function. In functional terms, it is the spatial domain version (i.e., the inverse Fourier transform) of the Optical transfer function, optical transfer function (OTF) of an imaging system. It is a useful concept in Fourier optics, astronomy, astronomical imaging, medical imaging, electron microscope, electron microscopy and other imaging techniques such as dimension, 3D microscopy (like in confocal laser scanning microscopy) and fluorescence microscopy. The degree of spreading (blurring) in the image of a point ob ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Optical Sectioning
Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome. Many different techniques for optical sectioning are used and several microscopy techniques are specifically designed to improve the quality of optical sectioning. Good optical sectioning, often referred to as good depth or z resolution, is popular in modern microscopy as it allows the three-dimensional reconstruction of a sample from images captured at different focal planes. Optical sectioning in traditional light microscopes In an ideal microscope, only light from the focal plane would be allowed to reach the detector (typically an observer or a CCD) producing a clear image of the plane of the sample the microscope is focused on. Unfortunately a microscope is not this specific and light from sources outside the focal plane also reaches the dete ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Raman Microscope
The Raman microscope is a laser-based microscopic device used to perform Raman spectroscopy.''Microscopical techniques in the use of the molecular optics laser examiner Raman microprobe'', by M. E. Andersen, R. Z. Muggli, Analytical Chemistry, 1981, 53 (12), pp 1772–177/ref> The term MOLE (molecular optics laser examiner) is used to refer to the Raman-based microprobe. The technique used is named after C. V. Raman, who discovered the scattering properties in liquids. Configuration The Raman microscope begins with a standard optical microscope, and adds an excitation laser, laser rejection filters, a spectrometer or monochromator, and an optical sensitive detector such as a charge-coupled device (CCD), or photomultiplier tube, (PMT). Traditionally Raman microscopy was used to measure the Raman spectrum of a point on a sample, more recently the technique has been extended to implement Raman spectroscopy for direct chemical imaging over the whole field of view on a 3D sample. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
