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Spot Analysis
Spot analysis, spot test analysis, or spot test is a chemical test, a simple and efficient technique where analytic assays are executed in only one, or a few drops, of a chemical solution, preferably in a great piece of filter paper, without using any sophisticated instrumentation. The development and popularization of the test is credited to Fritz Feigl. A spot test or spot assay can also refer to a test often used in microbiology. Chemistry The foundations of Feigl's work on spot analysis were the works of Hugo Schiff (the earliest publication about "spot test" was Shiff's detection of uric acid in 1859) and of Christian Friedrich Schonberg and Friedrich Goppelsröder on capillary analysis. On the occasion of Feigl's 70th birthday the Chemical Society of Midland sponsored a symposium in 1952, attended by 500 scientists from 24 countries, in which all plenary sessions were related to spot tests. The test uses the qualitative characteristics of colored compounds to account for ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chemical Test
In chemistry, a chemical test is a qualitative property, qualitative or Quantitative property, quantitative procedure designed to identify, quantify, or characterise a chemical compound or substituent, chemical group. Purposes Chemical testing might have a variety of purposes, such as to: * Determine if, or verify that, the requirements of a specification, regulation, or contract are met * Decide if a new product development program is on track: Demonstrate proof of concept * Demonstrate the utility of a proposed patent * Determine the interactions of a sample with other known substances * Determine the composition of a sample * Provide Technical standard, standard data for other scientific, medical, and Quality assurance functions * Validate suitability for end-use * Provide a basis for Technical communication * Provide a technical means of comparison of several options * Provide evidence in legal proceedings Biochemical tests * Clinistrips quantitatively test for sugar in uri ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chemical Tests
A chemical substance is a form of matter having constant chemical composition and characteristic properties. Some references add that chemical substance cannot be separated into its constituent elements by physical separation methods, i.e., without breaking chemical bonds. Chemical substances can be simple substances (substances consisting of a single chemical element), chemical compounds, or alloys. Chemical substances are often called 'pure' to set them apart from mixtures. A common example of a chemical substance is pure water; it has the same properties and the same ratio of hydrogen to oxygen whether it is isolated from a river or made in a laboratory. Other chemical substances commonly encountered in pure form are diamond (carbon), gold, table salt (sodium chloride) and refined sugar (sucrose). However, in practice, no substance is entirely pure, and chemical purity is specified according to the intended use of the chemical. Chemical substances exist as solids, liquids, g ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Touchstone (assaying Tool)
file:Pierre de touche.jpg, Touchstone set A touchstone is a small tablet of dark stone such as slate or lydite, used for Metallurgical assay, assaying precious metal alloys. It has a finely grained surface on which soft metals leave a visible trace. History The touchstone was used during the Indus Valley civilization#Chronology, Harappa period of the Indus Valley civilization ca. 2600–1900 BC for testing the purity of soft metals. It was also used in Ancient Greece. The touchstone allowed anyone to easily and quickly determine the purity of a metal sample. This, in turn, led to the widespread adoption of gold as a standard of exchange. Although mixing gold with less expensive materials was common in coinage, using a touchstone one could easily determine the quantity of gold in the coin, and thereby calculate its intrinsic worth. Operation Drawing a line with gold on a touchstone will leave a visible trace. Because different alloys of gold have different colours (see gold#Co ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biotechnology Reports
Biotechnology is the integration of natural sciences and engineering sciences in order to achieve the application of organisms, cells, parts thereof and molecular analogues for products and services. The term ''biotechnology'' was first used by Károly Ereky in 1919, meaning the production of products from raw materials with the aid of living organisms. Definition The concept of biotechnology encompasses a wide range of procedures for modifying living organisms according to human purposes, going back to domestication of animals, cultivation of the plants, and "improvements" to these through breeding programs that employ artificial selection and hybridization. Modern usage also includes genetic engineering as well as cell and tissue culture technologies. The American Chemical Society defines biotechnology as the application of biological organisms, systems, or processes by various industries to learning about the science of life and the improvement of the value of materials ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Two-hybrid Screening
Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively. The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the DNA-binding domain (DBD or often also abbreviated as BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay. History Pioneered by Stanley Fields and Ok-Kyu Song in 1989, the technique was originally designed to detect protein–protein ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Protein–protein Interaction
Protein–protein interactions (PPIs) are physical contacts of high specificity established between two or more protein molecules as a result of biochemical events steered by interactions that include electrostatic forces, hydrogen bonding and the hydrophobic effect. Many are physical contacts with molecular associations between chains that occur in a cell or in a living organism in a specific biomolecular context. Proteins rarely act alone as their functions tend to be regulated. Many molecular processes within a cell are carried out by molecular machines that are built from numerous protein components organized by their PPIs. These physiological interactions make up the so-called interactomics of the organism, while aberrant PPIs are the basis of multiple aggregation-related diseases, such as Creutzfeldt–Jakob and Alzheimer's diseases. PPIs have been studied with many methods and from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal trans ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
High-throughput Screening
High-throughput screening (HTS) is a method for scientific experimentation especially used in drug discovery and relevant to the fields of biology, materials science and chemistry. Using robotics, data processing/control software, liquid handling devices, and sensitive detectors, high-throughput screening allows a researcher to quickly conduct millions of chemical, genetic, or pharmacological tests. Through this process one can quickly recognize active compounds, antibodies, or genes that modulate a particular biomolecular pathway. The results of these experiments provide starting points for drug design and for understanding the noninteraction or role of a particular location. Assay plate preparation The key labware or testing vessel of HTS is the microtiter plate, which is a small container, usually disposable and made of plastic, that features a grid of small, open divots called ''wells''. In general, microplates for HTS have either 96, 192, 384, 1536, 3456 or 6144 wells. The ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spot Assay Yeast Cells
Spot or SPOT may refer to: Places * Spot, North Carolina, a community in the United States * The Spot, New South Wales, a locality in Sydney, Australia * South Pole Traverse, sometimes called the South Pole Overland Traverse People * Spot (producer), the house producer and engineer for the label SST Records * Spot (rapper) * Spot Collins (1922-1996), American football player and coach * Jerry Chamberlain (known as "Spot"), the guitarist for the rock group the Swirling Eddies * Scott Draves (known as "Spot"), digital artist and VJ * Moondog Spot, a ring name for professional wrestler Larry Booker Advertising * Radio spot, an over-the-air advertisement * TV spot, a televised advertisement * Underwriting spot, an announcement made on public broadcasting outlets, especially in the United States, in exchange for funding Animals * Spot, a dog that remained faithful after his master's death, described in the List of individual dogs * Spot Fetcher, a dog owned by U.S. President Geor ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fritz Feigl
Fritz Feigl (15 May 1891 – 23 January 1971) was a Jewish Austrian-born chemist. He taught at the University of Brazil. Biography Feigl was born and studied in Vienna, but owing to his military service in the First World War he had to interrupt his studies. He received his Ph.D. for work with Wilhelm Schlenk in 1920. After his habilitation in 1928 he became a professor at the University of Vienna. He was forced to retire after the Nazi occupation of Austria in 1938. Feigl was able to get to Belgium and work there. After the occupation of Belgium he was imprisoned in a concentration camp, but was able to reach Portugal and from there Brazil in 1940. He worked at the University of Rio de Janeiro and became a Brazilian citizen in 1944. Contributions Fritz Feigl is the creator of "spot analysis" (spot test), a simple and efficient technique where analytic assays are executed in only one, or a few drops, of a chemical solution, preferably in a great piece of filter paper, wi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chemical Society Of Midland
A chemical substance is a form of matter having constant chemical composition and characteristic properties. Some references add that chemical substance cannot be separated into its constituent elements by physical separation methods, i.e., without breaking chemical bonds. Chemical substances can be simple substances (substances consisting of a single chemical element), chemical compounds, or alloys. Chemical substances are often called 'pure' to set them apart from mixtures. A common example of a chemical substance is pure water; it has the same properties and the same ratio of hydrogen to oxygen whether it is isolated from a river or made in a laboratory. Other chemical substances commonly encountered in pure form are diamond (carbon), gold, table salt ( sodium chloride) and refined sugar ( sucrose). However, in practice, no substance is entirely pure, and chemical purity is specified according to the intended use of the chemical. Chemical substances exist as solids, liqu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Capillary Analysis
A capillary is a small blood vessel from 5 to 10 micrometres (μm) in diameter. Capillaries are composed of only the tunica intima, consisting of a thin wall of simple squamous endothelial cells. They are the smallest blood vessels in the body: they convey blood between the arterioles and venules. These microvessels are the site of exchange of many substances with the interstitial fluid surrounding them. Substances which cross capillaries include water, oxygen, carbon dioxide, urea, glucose, uric acid, lactic acid and creatinine. Lymph capillaries connect with larger lymph vessels to drain lymphatic fluid collected in the microcirculation. During early embryonic development, new capillaries are formed through vasculogenesis, the process of blood vessel formation that occurs through a '' de novo'' production of endothelial cells that then form vascular tubes. The term '' angiogenesis'' denotes the formation of new capillaries from pre-existing blood vessels and already present ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
