Soft Rot Enterobacteriaceae (SRE) Small RNAs
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Soft Rot Enterobacteriaceae (SRE) Small RNAs
Soft rot ''Enterobacteriaceae'' (SRE) (spanning the genera ''Erwinia'', ''Pectobacterium, Dickeya,'' and ''Pantoea''), are ubiquitous necrotrophic bacterial pathogens that infect a large number of different plant species worldwide, including economically important crops. When they live in soil outside of their plant hosts they starve and have to adapt to this new condition. By using strand-specific RNA-seq analysis and ''in silico'' sRNA predictions 137 small RNAs candidates were identified in ''Pectobacterium atrosepticum'' under starvation conditions. This suggests that sRNAs play roles in bacterial adaptive response. The expression of 9 novel candidate sRNAs was validated by RT-PCR. Those included antisense RNA Antisense RNA (asRNA), also referred to as antisense transcript, natural antisense transcript (NAT) or antisense oligonucleotide, is a single stranded RNA that is complementary to a protein coding messenger RNA (mRNA) with which it hybridizes, and ...s and UTR regions ...
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Erwinia
''Erwinia'' is a genus of Enterobacterales bacteria containing mostly plant pathogenic species which was named for the famous plant pathologist, Erwin Frink Smith. It contains Gram-negative bacteria related to ''Escherichia coli'', ''Shigella'', ''Salmonella'', and ''Yersinia''. They are primarily rod-shaped bacteria. Many infect woody plants. A well-known member of this genus is the species '' E. amylovora'', which causes fire blight on apples, pears, and other Rosaceae crops; ''E. tracheiphila'', though, causes bacterial wilt of cucurbits. Other familiar species, such as '' E. carotovora'' (another major cause of plant diseases), are more distantly related to the fire blight bacterium, and have been moved to genera ''Brenneria'', ''Dickeya'', and '' Pectobacterium''. ''Erwinia aphidocola'' and ''E. persicina'' species were both observed to be present within the floral nectar microbial community of seven different orchid (''Epipactis'') flower species. ''E. aphidicola'' appear ...
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Pectobacterium
''Pectobacterium'' is a bacterial genus of the family Pectobacteriaceae; it used to be a member of the genus ''Erwinia'', which was split into three genera: ''Erwinia'', ''Pectobacterium'', and ''Brenneria''. Species include ''Pectobacterium carotovorum ''Pectobacterium carotovorum'' is a bacterium of the family Pectobacteriaceae; it used to be a member of the genus ''Erwinia''. The species is a plant pathogen with a diverse host range, including many agriculturally and scientifically importa ...''. References External links Pectobacterium page on LPSN Bacteria genera Enterobacterales {{gammaproteobacteria-stub ...
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Dickeya
''Dickeya'' is a genus of the family Pectobacteriaceae that consists mainly of pathogens from herbaceous plants. Dickeya is the result of the reclassification of 75 strains of ''Pectobacterium chrysanthemi'', as well as ''Brenneria paradisiaca'' CFBP 4178, into a new genus. The genus is named for American phytopathologist Robert S. Dickey. Several species in this genus, such as ''Dickeya dadantii'', are known phytopathogens. Species now placed here include: * ''Dickeya aquatica'' * ''Dickeya chrysanthemi'' * ''Dickeya dadantii'' (''D. chrysanthemi'') * ''Dickeya dianthicola'' * ''Dickeya dieffenbachiae'' * ''Dickeya paradisiaca'' * ''Dickeya solani'' * ''Dickeya zeae ''Dickeya'' is a genus of the family Pectobacteriaceae that consists mainly of pathogens from herbaceous plants. Dickeya is the result of the reclassification of 75 strains of ''Pectobacterium chrysanthemi'', as well as '' Brenneria paradisiaca ...'' References External links * *NCBI Taxonomy Bacteria ...
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Pantoea
''Pantoea'' is a genus of Gram-negative bacteria of the family Erwiniaceae, recently separated from the genus ''Enterobacter''. This genus includes at least 20 species. ''Pantoea'' bacteria are yellow pigmented, ferment lactose, are motile, and form mucoid colonies. Some species show quorum sensing ability that could drive different gene expression, hence controlling certain physiological activities. Levan produced by ''Pantoea agglomerans'' ZMR7 was reported to decrease the viability of rhabdomyosarcoma (RD) and breast cancer (MDA) cells compared with untreated cancer cells. In addition, it has high antiparasitic activity against the promastigote of ''Leishmania tropica.'' Species *''Pantoea agglomerans'' is the most common ''Pantoea'' species recovered from humans and an opportunistic pathogen associated with contaminated catheter In medicine, a catheter (/ˈkæθətər/) is a thin tubing (material), tube made from medical grade materials serving a broad range of functio ...
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Small RNA
Small RNA (sRNA) are polymeric RNA molecules that are less than 200 nucleotides in length, and are usually non-coding Non-coding DNA (ncDNA) sequences are components of an organism's DNA that do not encode protein sequences. Some non-coding DNA is transcribed into functional non-coding RNA molecules (e.g. transfer RNA, microRNA, piRNA, ribosomal RNA, and regula .... RNA silencing is often a function of these molecules, with the most common and well-studied example being RNA interference (RNAi), in which endogenously expressed microRNA (miRNA) or exogenously derived small interfering RNA (siRNA) induces the degradation of complementarity (molecular biology), complementary messenger RNA. Other classes of small RNA have been identified, including piwi-interacting RNA (piRNA) and its subspecies rasiRNA, repeat associated small interfering RNA (rasiRNA). Small RNA "is unable to induce RNAi alone, and to accomplish the task it must form the core of the RNA–protein complex termed the ...
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Pectobacterium Atrosepticum
''Pectobacterium atrosepticum'' is a species of bacterium. It is a plant pathogen causing blackleg of potato. Its type strain is CFBP 1526T (=LMG 2386T =NCPPB 549TICMP 1526T. Its genome has been sequenced. References Further reading * Liu, Hui, et al. "Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum." PLoS Pathogens 4.6 (2008): e1000093. * External links LPSNType strain of ''Pectobacterium atrosepticum'' at Bac''Dive'' - the Bacterial Diversity Metadatabase Enterobacterales Bacterial plant pathogens and diseases Potato diseases Bacteria described in 2003 {{Enterobacterales-stub ...
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Antisense RNA
Antisense RNA (asRNA), also referred to as antisense transcript, natural antisense transcript (NAT) or antisense oligonucleotide, is a single stranded RNA that is complementary to a protein coding messenger RNA (mRNA) with which it hybridizes, and thereby blocks its translation into protein. asRNAs (which occur naturally) have been found in both prokaryotes and eukaryotes, and can be classified into short (200 nucleotides) non-coding RNAs (ncRNAs). The primary function of asRNA is regulating gene expression. asRNAs may also be produced synthetically and have found wide spread use as research tools for gene knockdown. They may also have therapeutic applications. Discovery and history in drug development Some of the earliest asRNAs were discovered while investigating functional proteins. An example was micF asRNA. While characterizing the outer membrane porin in ''E.coli'', some of the promoter clones observed were capable of repressing the expression of other membrane porin s ...
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Untranslated Region
In molecular genetics, an untranslated region (or UTR) refers to either of two sections, one on each side of a coding sequence on a strand of mRNA. If it is found on the 5' side, it is called the 5' UTR (or leader sequence), or if it is found on the 3' side, it is called the 3' UTR (or trailer sequence). mRNA is RNA that carries information from DNA to the ribosome, the site of protein synthesis (translation) within a cell. The mRNA is initially transcribed from the corresponding DNA sequence and then translated into protein. However, several regions of the mRNA are usually not translated into protein, including the 5' and 3' UTRs. Although they are called untranslated regions, and do not form the protein-coding region of the gene, uORFs located within the 5' UTR can be translated into peptides. The 5' UTR is upstream from the coding sequence. Within the 5' UTR is a sequence that is recognized by the ribosome which allows the ribosome to bind and initiate translation. Th ...
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