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RAD-seq
Restriction site associated DNA (RAD) markers are a type of genetic marker which are useful for association mapping, Quantitative trait locus, QTL-mapping, population genetics, ecological genetics and evolutionary genetics. The use of RAD markers for genetic mapping is often called RAD mapping. An important aspect of RAD markers and mapping is the process of isolating RAD tags, which are the DNA sequences that immediately flank each instance of a particular restriction site of a restriction enzyme throughout the genome. Once RAD tags have been isolated, they can be used to identify and genotype DNA sequence polymorphisms mainly in form of Single-nucleotide polymorphism, single nucleotide polymorphisms (SNPs). Polymorphisms that are identified and genotyped by isolating and analyzing RAD tags are referred to as RAD markers. Although genotyping by sequencing presents an approach similar to the RAD-seq method, they differ in some substantial ways. Isolation of RAD tags The use of the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Genotyping By Sequencing
In the field of DNA sequencing, genetic sequencing, genotyping by sequencing, also called GBS, is a method to discover Single-nucleotide polymorphism, single nucleotide polymorphisms (SNP) in order to perform genotyping studies, such as genome-wide association studies (Genome-wide association study, GWAS). GBS uses restriction enzymes to reduce genome complexity and genotype multiple DNA samples. After digestion, Polymerase chain reaction, PCR is performed to increase fragments pool and then GBS libraries are sequenced using next generation sequencing technologies, usually resulting in about 100bp single-end reads. It is relatively inexpensive and has been used in plant breeding. Although GBS presents an approach similar to Restriction site associated DNA markers, restriction-site-associated DNA sequencing (RAD-seq) method, they differ in some substantial ways. Methods GBS is a robust, simple, and affordable procedure for SNP discovery and mapping. Overall, this approach reduces ge ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Genotyping By Sequencing
In the field of DNA sequencing, genetic sequencing, genotyping by sequencing, also called GBS, is a method to discover Single-nucleotide polymorphism, single nucleotide polymorphisms (SNP) in order to perform genotyping studies, such as genome-wide association studies (Genome-wide association study, GWAS). GBS uses restriction enzymes to reduce genome complexity and genotype multiple DNA samples. After digestion, Polymerase chain reaction, PCR is performed to increase fragments pool and then GBS libraries are sequenced using next generation sequencing technologies, usually resulting in about 100bp single-end reads. It is relatively inexpensive and has been used in plant breeding. Although GBS presents an approach similar to Restriction site associated DNA markers, restriction-site-associated DNA sequencing (RAD-seq) method, they differ in some substantial ways. Methods GBS is a robust, simple, and affordable procedure for SNP discovery and mapping. Overall, this approach reduces ge ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
DNA Sequencing
DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Knowledge of DNA sequences has become indispensable for basic biological research, DNA Genographic Projects and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. Comparing healthy and mutated DNA sequences can diagnose different diseases including various cancers, characterize antibody repertoire, and can be used to guide patient treatment. Having a quick way to sequence DNA allows for faster and more individualized medical care to be administered, and for more organisms to be identified and cataloged. The rapid speed of sequencing attained with modern D ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Arthropod
Arthropods (, (gen. ποδός)) are invertebrate animals with an exoskeleton, a Segmentation (biology), segmented body, and paired jointed appendages. Arthropods form the phylum Arthropoda. They are distinguished by their jointed limbs and Arthropod cuticle, cuticle made of chitin, often Mineralization (biology), mineralised with calcium carbonate. The arthropod body plan consists of segments, each with a pair of appendages. Arthropods are bilaterally symmetrical and their body possesses an exoskeleton, external skeleton. In order to keep growing, they must go through stages of moulting, a process by which they shed their exoskeleton to reveal a new one. Some species have wings. They are an extremely diverse group, with up to 10 million species. The haemocoel, an arthropod's internal cavity, through which its haemolymph – analogue of blood – circulates, accommodates its interior Organ (anatomy), organs; it has an open circulatory system. Like their exteriors, the internal or ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Regent Honeyeater
The regent honeyeater (''Anthochaera phrygia'') is a critically endangered bird endemic to southeastern Australia. It is commonly considered a flagship species within its range, with the efforts going into its conservation having positive effects on many other species that share its habitat. Recent genetic research suggests it is closely related to the wattlebirds. Taxonomy First described by the English naturalist George Shaw in 1794, the regent honeyeater was moved to ''Anthochaera'' in 1827 by the naturalists Nicholas Aylward Vigors and Thomas Horsfield. It was known as ''Xanthomyza phrygia'' for many years, the genus erected by William John Swainson in 1837. DNA analysis shows that its ancestry is in fact nested within the wattlebird genus ''Anthochaera''. The ancestor of the regent honeyeater split from a lineage that gave rise to the red and yellow wattlebirds. The little and western wattlebirds arose from another lineage that diverged earlier. The generic name ''Anthocha ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Palearctic Realm
The Palearctic or Palaearctic is the largest of the eight biogeographic realms of the Earth. It stretches across all of Eurasia north of the foothills of the Himalayas, and North Africa. The realm consists of several bioregions: the Euro-Siberian region; the Mediterranean Basin; the Sahara and Arabian Deserts; and Western, Central and East Asia. The Palaearctic realm also has numerous rivers and lakes, forming several freshwater ecoregions. The term 'Palearctic' was first used in the 19th century, and is still in use as the basis for zoogeographic classification. History In an 1858 paper for the ''Proceedings of the Linnean Society'', British zoologist Philip Sclater first identified six terrestrial zoogeographic realms of the world: Palaearctic, Aethiopian/Afrotropic, Indian/Indomalayan, Australasian, Nearctic, and Neotropical. The six indicated general groupings of fauna, based on shared biogeography and large-scale geographic barriers to migration. Alfred Wallace ad ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Oedaleus Decorus
''Oedaleus decorus'' is a species of band-winged grasshopper in the family Acrididae. It is found in the Palearctic The Palearctic or Palaearctic is the largest of the eight biogeographic realms of the Earth. It stretches across all of Eurasia north of the foothills of the Himalayas, and North Africa. The realm consists of several bioregions: the Euro-Sibe .... References External links * Oedipodinae Palearctic insects Insects described in 1825 {{oedipodinae-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Restriction Site
Restriction sites, or restriction recognition sites, are located on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes. These are generally palindromic sequences (because restriction enzymes usually bind as homodimers), and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby. Function For example, the common restriction enzyme EcoRI recognizes the palindromic sequence GAATTC and cuts between the G and the A on both the top and bottom strands. This leaves an overhang (an end-portion of a DNA strand with no attached complement) known as a sticky end on each end of AATT. The overhang can then be used to ligate in (see DNA ligase) a piece of DNA with a complementary overhang (another EcoRI-cut piece, for example). Some restriction enzymes cut DNA at a restriction site in a manner which leaves no overhang, called a blunt end. Blunt ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Museomics
Museomics is the study of genomic data obtained from ancient DNA (aDNA) and historic DNA (hDNA) specimens in museum collections. Early research in this area focused on short sequences of DNA from mitochondrial genes, but sequencing of whole genomes has become possible. Next-generation sequencing (NGS) and high-throughput sequencing (HTS) methods can be applied to the analysis of genetic datasets extracted from collections materials. Such techniques have been described as a "third revolution in sequencing technology". Like radiocarbon dating, the techniques of museomics are a transformative technology. Results are revising and sometimes overturning previously accepted theories about a wide variety of topics such as the domestication of the horse. Museum collections contain unique resources such as natural history specimens, which can be used for genome-scale examinations of species, their evolution, and their responses to environmental change. Ancient DNA provides a unique windo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sequence Homology
Sequence homology is the biological homology between DNA, RNA, or protein sequences, defined in terms of shared ancestry in the evolutionary history of life. Two segments of DNA can have shared ancestry because of three phenomena: either a speciation event (orthologs), or a duplication event (paralogs), or else a horizontal (or lateral) gene transfer event (xenologs). Homology among DNA, RNA, or proteins is typically inferred from their nucleotide or amino acid sequence similarity. Significant similarity is strong evidence that two sequences are related by evolutionary changes from a common ancestral sequence. Alignments of multiple sequences are used to indicate which regions of each sequence are homologous. Identity, similarity, and conservation The term "percent homology" is often used to mean "sequence similarity”, that is the percentage of identical residues (''percent identity''), or the percentage of residues conserved with similar physicochemical properties (' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Shotgun Sequencing
In genetics, shotgun sequencing is a method used for sequencing random DNA strands. It is named by analogy with the rapidly expanding, quasi-random shot grouping of a shotgun. The Sanger sequencing#Method, chain-termination method of DNA sequencing ("Sanger sequencing") can only be used for short DNA strands of 100 to 1000 base pairs. Due to this size limit, longer sequences are subdivided into smaller fragments that can be sequenced separately, and these sequences are sequence assembly, assembled to give the overall sequence. In shotgun sequencing, DNA is broken up randomly into numerous small segments, which are sequenced using the chain termination method to obtain ''reads''. Multiple overlapping reads for the target DNA are obtained by performing several rounds of this fragmentation and sequencing. Computer programs then use the overlapping ends of different reads to assemble them into a continuous sequence. Shotgun sequencing was one of the precursor technologies that was res ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Population Differentiation
Human genetic variation is the genetic differences in and among populations. There may be multiple variants of any given gene in the human population (alleles), a situation called polymorphism. No two humans are genetically identical. Even monozygotic twins (who develop from one zygote) have infrequent genetic differences due to mutations occurring during development and gene copy-number variation. Differences between individuals, even closely related individuals, are the key to techniques such as genetic fingerprinting. As of 2017, there are a total of 324 million known variants from sequenced human genomes. As of 2015, the typical difference between an individual's genome and the reference genome was estimated at 20 million base pairs (or 0.6% of the total of 3.2 billion base pairs). Comparatively speaking, humans are a genetically homogenous species. Although a small number of genetic variants are found more frequently in certain geographic regions or in people with ance ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
