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RcsR1 Small RNA
RcsR1 (rhizobial cold and salinity stress riboregulator 1) trans-acting sRNA, formerly known as SmelC587, is a stress-related riboregulator, conserved in ''Sinorhizobium'', '' Rhizobium'' and ''Agrobacterium''. It contains highly conserved stem-loops involved in the interaction with several target mRNAs (PhoR, MotE, anti-σE1, GntR, FgA, TrpC). In '' Sinorhizobium meliloti'' RcsR1 less conserved central region is responsible for the species-specific interaction with the 5’UTR of autoinducer synthase encoding mRNA ''sinI''. The interaction negatively influences ''sinI'' translation. See also * SuhB * EcpR1 sRNA EcpR1 (elongated cell phenotype RNA1) is a ''trans-''encoded small non-coding RNA in the plant-symbiotic '' Sinorhizobium meliloti'', previously named SmelC291, SmrC10, or Sra33. According to its overproduction phenotype it was renamed Elongated Ce ... References {{reflist Non-coding RNA ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Secondary Structure
Protein secondary structure is the three dimensional conformational isomerism, form of ''local segments'' of proteins. The two most common Protein structure#Secondary structure, secondary structural elements are alpha helix, alpha helices and beta sheets, though beta turns and omega loops occur as well. Secondary structure elements typically spontaneously form as an intermediate before the protein protein folding, folds into its three dimensional protein tertiary structure, tertiary structure. Secondary structure is formally defined by the pattern of hydrogen bonds between the Amine, amino hydrogen and carboxyl oxygen atoms in the peptide backbone chain, backbone. Secondary structure may alternatively be defined based on the regular pattern of backbone Dihedral angle#Dihedral angles of proteins, dihedral angles in a particular region of the Ramachandran plot regardless of whether it has the correct hydrogen bonds. The concept of secondary structure was first introduced by Kaj Ulrik ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sequence Conservation
In evolutionary biology, conserved sequences are identical or similar sequences in nucleic acids ( DNA and RNA) or proteins across species ( orthologous sequences), or within a genome ( paralogous sequences), or between donor and receptor taxa ( xenologous sequences). Conservation indicates that a sequence has been maintained by natural selection. A highly conserved sequence is one that has remained relatively unchanged far back up the phylogenetic tree, and hence far back in geological time. Examples of highly conserved sequences include the RNA components of ribosomes present in all domains of life, the homeobox sequences widespread amongst Eukaryotes, and the tmRNA in Bacteria. The study of sequence conservation overlaps with the fields of genomics, proteomics, evolutionary biology, phylogenetics, bioinformatics and mathematics. History The discovery of the role of DNA in heredity, and observations by Frederick Sanger of variation between animal insulins in 1949, promp ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacteria
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationsh ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rhizobium
''Rhizobium'' is a genus of Gram-negative soil bacteria that fix nitrogen. ''Rhizobium'' species form an endosymbiotic nitrogen-fixing association with roots of (primarily) legumes and other flowering plants. The bacteria colonize plant cells within root nodules, where they convert atmospheric nitrogen into ammonia using the enzyme nitrogenase and then provide organic nitrogenous compounds such as glutamine or ureides to the plant. The plant, in turn, provides the bacteria with organic compounds made by photosynthesis. This mutually beneficial relationship is true of all of the rhizobia, of which the genus ''Rhizobium'' is a typical example. ''Rhizobium'' is also capable to solubilize phosphorus. History Martinus Beijerinck was the first to isolate and cultivate a microorganism from the nodules of legumes in 1888. He named it ''Bacillus radicicola'', which is now placed in ''Bergey's Manual of Determinative Bacteriology'' under the genus ''Rhizobium''. Research ''Rhizobium'' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Agrobacterium
''Agrobacterium'' is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants. ''Agrobacterium tumefaciens'' is the most commonly studied species in this genus. ''Agrobacterium'' is well known for its ability to transfer DNA between itself and plants, and for this reason it has become an important tool for genetic engineering. Nomenclatural History Leading up to the 1990s, the genus ''Agrobacterium'' was used as a wastebasket taxon. With the advent of 16S sequencing, many ''Agrobacterium'' species (especially the marine species) were reassigned to genera such as ''Ahrensia'', ''Pseudorhodobacter'', ''Ruegeria'', and ''Stappia''. The remaining ''Agrobacterium'' species were assigned to three biovars: biovar 1 (''Agrobacterium tumefaciens''), biovar 2 (''Agrobacterium rhizogenes''), and biovar 3 (''Agrobacterium vitis''). In the early 2000s, ''Agrobacterium'' was synonymized with the genus ''Rhizobium''. This move pr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Highly Conserved
In evolutionary biology, conserved sequences are identical or similar sequences in nucleic acids ( DNA and RNA) or proteins across species ( orthologous sequences), or within a genome ( paralogous sequences), or between donor and receptor taxa ( xenologous sequences). Conservation indicates that a sequence has been maintained by natural selection. A highly conserved sequence is one that has remained relatively unchanged far back up the phylogenetic tree, and hence far back in geological time. Examples of highly conserved sequences include the RNA components of ribosomes present in all domains of life, the homeobox sequences widespread amongst Eukaryotes, and the tmRNA in Bacteria. The study of sequence conservation overlaps with the fields of genomics, proteomics, evolutionary biology, phylogenetics, bioinformatics and mathematics. History The discovery of the role of DNA in heredity, and observations by Frederick Sanger of variation between animal insulins in 1949, prompted ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sinorhizobium Meliloti
''Ensifer meliloti'' (formerly ''Rhizobium meliloti'' and ''Sinorhizobium meliloti'') are an aerobic, Gram-negative, and diazotrophic species of bacteria. ''S. meliloti'' are motile and possess a cluster of peritrichous flagella. ''S. meliloti'' fix atmospheric nitrogen into ammonia for their legume symbionts, such as alfalfa. ''S. meliloti'' forms a symbiotic relationship with legumes from the genera ''Medicago'', ''Melilotus'' and ''Trigonella'', including the model legume ''Medicago truncatula''. This symbiosis promotes the development of a plant organ, termed a root nodule. Because soil often contains a limited amount of nitrogen for plant use, the symbiotic relationship between ''S. meliloti'' and their legume hosts has agricultural applications. These techniques reduce the need for inorganic nitrogenous fertilizers. Symbiosis Symbiosis between ''S. meliloti'' and its legume hosts begins when the plant secretes an array of betaines and flavonoids into the rhizosphere: 4,4†... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Autoinducer Synthase
Acyl-homoserine-lactone synthase () is an enzyme with systematic name ''acyl-(acyl-carrier protein):S-adenosyl-L-methionine acyltranserase (lactone-forming, methylthioadenosine-releasing)''. This enzyme catalyses the following chemical reaction : acyl- cyl-carrier protein+ S-adenosyl-L-methionine \rightleftharpoons cyl-carrier protein+ S-methyl-5'-thioadenosine + N-acyl-L-homoserine lactone Acyl-homoserine lactones (AHLs) are produced by a number of bacterial species and are used by them to regulate the expression of virulence genes in a process known as quorum-sensing In biology, quorum sensing or quorum signalling (QS) is the ability to detect and respond to cell population density by gene regulation. As one example, QS enables bacteria to restrict the expression of specific genes to the high cell densities at .... Alternate names ''acyl-homoserine lactone synthase'', ''acyl homoserine lactone synthase'', ''acyl-homoserinelactone synthase'', ''acylhomoserine lactone ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
SuhB
''suhB'', also known as ''mmgR'' (makes more granules regulator), is a non-coding RNA found multiple times in the ''Agrobacterium tumefaciens'' genome and related alpha-proteobacteria. Other non-coding RNAs uncovered in the same analysis include '' speF'', '' ybhL'', ''metA'', and ''serC''. Several studies in '' Sinorhizobium meliloti'' showed that the ''suhB'' element is indeed a non-coding RNA. It was first detected by Northern blot and called ''Sm8RNA'', then in an RNAseq study and referred to as ''SmelC689''. The mutant (lacking the small RNA) phenotype's cytoplasm contains a higher content of polyhydroxybutyrate (PBH) storage granules than the wild type strain. The sRNA is required to limit the PBH intracellular accumulation when the nitrogen-fixing '' Sinorhizobium meliloti'' is converting surplus carbon to nitrogen his needs to be modified, carbon cannot be converted to nitrogen/sup>. Further study confirmed that ''suhB'' fine-tunes the regulation of PBH storage. North ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
EcpR1 SRNA
EcpR1 (elongated cell phenotype RNA1) is a ''trans-''encoded small non-coding RNA in the plant-symbiotic '' Sinorhizobium meliloti'', previously named SmelC291, SmrC10, or Sra33. According to its overproduction phenotype it was renamed Elongated Cell Phenotype RNA1. Induced by stress, EcpR1 negatively regulates cell cycle master regulatory genes ''dnaA'' and ''gcrA'' at post-transcriptional level by base pairing between its strongly conserved GC-rich loop and the target mRNAs. It is suggested that EcpR1 connects stress adaptation and cell cycle progression. See also * SuhB ''suhB'', also known as ''mmgR'' (makes more granules regulator), is a non-coding RNA found multiple times in the ''Agrobacterium tumefaciens'' genome and related alpha-proteobacteria. Other non-coding RNAs uncovered in the same analysis include ... * RcsR1 small RNA Further reading {{Reflist Non-coding RNA ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
