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Rathayibacter Toxicus
''Rathayibacter toxicus'' is a phytopathogenic bacterium known for causing annual ryegrass toxicity (ARGT) commonly found in South Australia, South and Western Australia. Etymology The genus ''Rathayibacter'' is an homage to E. Rathay, the plant pathologist who first isolated strains of the genus combined with the suffix -bacter meaning "rod" in Latin. The species name, ''toxicus'', stems from the Latin word meaning "poison", due to ''Rathayibacter toxicus'''s ability to produce Corynebacterium, corynetoxins. Taxonomy ''Rathayibacter toxicus'' has been previously classified as "''Corynebacterium'' sp.", "''Corynebacterium rathayi''", "''Clavibacter'' sp.", "''Clavibacter rathayi''", and "''Clavibacter toxicus.''" The organism is a member of the family Microbacteriaceae. ''Microbacteriaceae'' contains twenty-eight other genera, though a distinct clade is formed between genus ''Rathayibacter'' and genus ''Clavibacter.'' Genera that are closer related to ''Rathayibacter'' are ...
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Bacterium
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationshi ...
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Anguina (nematode)
''Anguina'' (seed-gall nematode, seed and leaf gall nematode, seed gall nematode, shoot gall nematode) is a genus of plant pathogenic nematodes. Species In addition to the type species: * ''Anguina tritici ''Anguina tritici'' (ear-cockle nematode, seed-gall nematode, seed and leaf gall nematode, wheat gall nematode, wheat seed gall nematode, wheat seed-gall nematode, wheat seed and leaf gall nematode) is a plant pathogenic nematode. History and ...'' (Steinbuch, 1799) Filipjev, 1936 species include: ;;Former species: * ''Anguina lolii'' Price, 1973 now a syn. ''Anguina agrostis'' * ''Anguina millefolii'' now ''Subanguina millefolii'' (Low, 1874) Brzeski, 1981 * ''Anguina phalaridis'' (Steinbuch) Chizhov, 1980 now a syn. ''Anguina agrostis'' * ''Anguina poophila'' Kirjanova, 1952, now a syn. ''Anguina agrostis'' * ''Anguina tumefaciens'' now ''Subanguina tumefaciens'' (Cobb, 1932) Fortuner & Maggenti, 1987 References Agricultural pest nematodes Tylenchida Sec ...
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Bacteriophage
A bacteriophage (), also known informally as a ''phage'' (), is a duplodnaviria virus that infects and replicates within bacteria and archaea. The term was derived from "bacteria" and the Greek φαγεῖν ('), meaning "to devour". Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome, and may have structures that are either simple or elaborate. Their genomes may encode as few as four genes (e.g. MS2) and as many as hundreds of genes. Phages replicate within the bacterium following the injection of their genome into its cytoplasm. Bacteriophages are among the most common and diverse entities in the biosphere. Bacteriophages are ubiquitous viruses, found wherever bacteria exist. It is estimated there are more than 1031 bacteriophages on the planet, more than every other organism on Earth, including bacteria, combined. Viruses are the most abundant biological entity in the water column of the world's oceans, and the second largest component of biom ...
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Allozyme
Alloenzymes (or also called allozymes) are variant forms of an enzyme which differ structurally but not functionally from other allozymes coded for by different alleles at the same locus. These are opposed to isozymes, which are enzymes that perform the same function, but which are coded by genes located at different loci. Alloenzymes are common biological enzymes that exhibit high levels of functional evolutionary conservation throughout specific phyla and kingdoms. They are used by phylogeneticists as molecular markers to gauge evolutionary histories and relationships between different species. This can be done because allozymes do not have the same structure. They can be separated by capillary electrophoresis. However, some species are monomorphic for many of their allozymes which would make it difficult for phylogeneticists to assess the evolutionary histories of these species. In these instances, phylogeneticists would have to use another method to determine the evolutionary ...
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Serology
Serology is the scientific study of Serum (blood), serum and other body fluids. In practice, the term usually refers to the medical diagnosis, diagnostic identification of Antibody, antibodies in the serum. Such antibodies are typically formed in response to an infection (against a given microorganism), against other foreign proteins (in response, for example, to a Acute hemolytic transfusion reaction, mismatched blood transfusion), or to one's own proteins (in instances of autoimmune disease). In either case, the procedure is simple. Serological tests Serological tests are diagnostic methods that are used to identify antibodies and antigens in a patient's sample. Serological tests may be performed to diagnose infections and autoimmune illnesses, to check if a person has immunity (medical), immunity to certain diseases, and in many other situations, such as determining an individual's blood type. Serological tests may also be used in forensic serology to investigate crime scene evid ...
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Immunoassay
An immunoassay (IA) is a biochemical test that measures the presence or concentration of a macromolecule or a small molecule in a solution through the use of an antibody (usually) or an antigen (sometimes). The molecule detected by the immunoassay is often referred to as an "analyte" and is in many cases a protein, although it may be other kinds of molecules, of different sizes and types, as long as the proper antibodies that have the required properties for the assay are developed. Analytes in biological liquids such as serum or urine are frequently measured using immunoassays for medical and research purposes. Immunoassays come in many different formats and variations. Immunoassays may be run in multiple steps with reagents being added and washed away or separated at different points in the assay. Multi-step assays are often called separation immunoassays or heterogeneous immunoassays. Some immunoassays can be carried out simply by mixing the reagents and sample and making a p ...
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Peptidoglycan
Peptidoglycan or murein is a unique large macromolecule, a polysaccharide, consisting of sugars and amino acids that forms a mesh-like peptidoglycan layer outside the plasma membrane, the rigid cell wall (murein sacculus) characteristic of most bacteria (domain ''Bacteria''). The sugar component consists of alternating residues of β-(1,4) linked ''N''-acetylglucosamine (NAG) and ''N''-acetylmuramic acid (NAM). Attached to the ''N''-acetylmuramic acid is a oligopeptide chain made of three to five amino acids. The peptide chain can be cross-linked to the peptide chain of another strand forming the 3D mesh-like layer. Peptidoglycan serves a structural role in the bacterial cell wall, giving structural strength, as well as counteracting the osmotic pressure of the cytoplasm. This repetitive linking results in a dense peptidoglycan layer which is critical for maintaining cell form and withstanding high osmotic pressures, and it is regularly replaced by peptidoglycan production. Pep ...
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Cell Wall
A cell wall is a structural layer surrounding some types of cells, just outside the cell membrane. It can be tough, flexible, and sometimes rigid. It provides the cell with both structural support and protection, and also acts as a filtering mechanism. Cell walls are absent in many eukaryotes, including animals, but are present in some other ones like fungi, algae and plants, and in most prokaryotes (except mollicute bacteria). A major function is to act as pressure vessels, preventing over-expansion of the cell when water enters. The composition of cell walls varies between taxonomic group and species and may depend on cell type and developmental stage. The primary cell wall of land plants is composed of the polysaccharides cellulose, hemicelluloses and pectin. Often, other polymers such as lignin, suberin or cutin are anchored to or embedded in plant cell walls. Algae possess cell walls made of glycoproteins and polysaccharides such as carrageenan and agar that are absent ...
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Distilled Water
Distilled water is water that has been boiled into vapor and condensed back into liquid in a separate container. Impurities in the original water that do not boil below or near the boiling point of water remain in the original container. Thus, distilled water is a type of purified water. History Drinking water has been distilled from seawater since at least about AD 200, when the process was clearly described by Alexander of Aphrodisias. Its history predates this, as a passage in Aristotle's ''Meteorologica'' refers to the distillation of water. Captain Israel Williams of the ''Friendship'' (1797) improvised a way to distill water, which he described in his journal. Applications In chemical and biological laboratories, as well as in industry, in some appliances deionised water can be used instead of distilled water as a cheaper alternative. If exceptionally high-purity water is required, double distilled water is used. In general, non-purified water could cause or interfere wi ...
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Agar
Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from ogonori (''Gracilaria'') and "tengusa" (''Gelidiaceae''). As found in nature, agar is a mixture of two components, the linear polysaccharide agarose and a heterogeneous mixture of smaller molecules called agaropectin. It forms the supporting structure in the cell walls of certain species of algae and is released on boiling. These algae are known as agarophytes, belonging to the Rhodophyta (red algae) phylum. The processing of food-grade agar removes the agaropectin, and the commercial product is essentially pure agarose. Agar has been used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture media for microbiological work. Agar can be used as a laxative; an appetite suppressant; a vegan substitute for gelatin; a thickener for soups; in fruit preserves, ice cream, and other desser ...
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Yeast Extract
Yeast extracts consist of the cell contents of yeast without the cell walls; they are used as food additives or flavorings, or as nutrients for bacterial culture media. They are often used to create savory flavors and umami taste sensations, and can be found in a large variety of packaged food, including frozen meals, crackers, snack foods, gravy, stock and more. They are rich in B vitamins (but not B12). Yeast extracts and fermented foods contain glutamic acid (free glutamates), an amino acid which adds an umami flavor. Glutamic acid is found in meat, cheese, fungi (mushrooms and yeast), and vegetables—such as broccoli, and tomatoes. The heat-autolytic process to make yeast extract of the autolysate type was invented in the 19th century by Justus von Liebig. Yeast cells are heated until they rupture, then the cells' own digestive enzymes break their proteins down into simpler compounds (amino acids and peptides), a process called autolysis. The insoluble cell walls are the ...
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Caseine
Casein ( , from Latin ''caseus'' "cheese") is a family of related phosphoproteins ( αS1, aS2, β, κ) that are commonly found in mammalian milk, comprising about 80% of the proteins in cow's milk and between 20% and 60% of the proteins in human milk. Sheep and buffalo milk have a higher casein content than other types of milk with human milk having a particularly low casein content. Casein has a wide variety of uses, from being a major component of cheese, to use as a food additive. The most common form of casein is sodium caseinate. In milk, casein undergoes phase separation to form colloidal casein micelles, a type of secreted biomolecular condensate. As a food source, casein supplies amino acids, carbohydrates, and two essential elements, calcium and phosphorus. Composition Casein contains a high number of proline amino acids which hinder the formation of common secondary structural motifs of proteins. There are also no disulfide bridges. As a result, it has relatively ...
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