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Potentiometric Dyes
Voltage-sensitive dyes, also known as potentiometric dyes, are dyes which change their spectral properties in response to voltage changes. They are able to provide linear measurements of firing activity of single neurons, large neuronal populations or activity of myocytes. Many physiological processes are accompanied by changes in cell membrane potential which can be detected with voltage sensitive dyes. Measurements may indicate the site of action potential origin, and measurements of action potential velocity and direction may be obtained. Potentiometric dyes are used to monitor the electrical activity inside cell organelles where it is not possible to insert an electrode, such as the mitochondria and dendritic spine. This technology is especially powerful for the study of patterns of activity in complex multicellular preparations. It also makes possible the measurement of spatial and temporal variations in membrane potential along the surface of single cells. Types of dyes F ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dyes
A dye is a colored substance that chemically bonds to the substrate to which it is being applied. This distinguishes dyes from pigments which do not chemically bind to the material they color. Dye is generally applied in an aqueous solution and may require a mordant to improve the fastness of the dye on the fiber. There are two broad categories of dyes: natural and synthetic; Natural dyes are dyes extracted from plants, Insects, or minerals. The majority of natural dyes are vegetable dyes derived from plant sources such as roots, berries, bark, leaves, and wood, as well as other biological sources like fungi. Synthetic dyes are also referred to as "coal tar dyes" because they are derived from substances that, until recently, could only be extracted from coal tar. A synthetic dye consists of a chromophore and an auxochrome added to a benzene derivative. Both dyes and pigments are colored, because they absorb only some wavelengths of visible light. Dyes are usually soluble i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Magnetic Moment
In electromagnetism, the magnetic moment is the magnetic strength and orientation of a magnet or other object that produces a magnetic field. Examples of objects that have magnetic moments include loops of electric current (such as electromagnets), permanent magnets, elementary particles (such as electrons), various molecules, and many astronomical objects (such as many planets, some moons, stars, etc). More precisely, the term ''magnetic moment'' normally refers to a system's magnetic dipole moment, the component of the magnetic moment that can be represented by an equivalent magnetic dipole: a magnetic north and south pole separated by a very small distance. The magnetic dipole component is sufficient for small enough magnets or for large enough distances. Higher-order terms (such as the magnetic quadrupole moment) may be needed in addition to the dipole moment for extended objects. The magnetic dipole moment of an object is readily defined in terms of the torque that the objec ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Squid Giant Axon
The squid giant axon is the very large (up to 1.5 mm in diameter; typically around 0.5 mm) axon that controls part of the water jet propulsion system in squid. It was first described by L. W. Williams in 1909, but this discovery was forgotten until English zoologist and neurophysiologist J. Z. Young demonstrated the axon's function in the 1930s while working in the Stazione Zoologica in Naples, the Marine Biological Association in Plymouth and the Marine Biological Laboratory in Woods Hole. Squids use this system primarily for making brief but very fast movements through the water. On the underside of the squid's body, between the head and the mantle, is a siphon through which water can be rapidly expelled by the fast contractions of the body wall muscles of the animal. This contraction is initiated by action potentials in the giant axon. Action potentials travel faster in a larger axon than a smaller one, and squid have evolved the giant axon to improve the speed ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spatial Filter
A spatial filter is an optical device which uses the principles of Fourier optics to alter the structure of a beam of light or other electromagnetic radiation, typically coherent laser light. Spatial filtering is commonly used to "clean up" the output of lasers, removing aberrations in the beam due to imperfect, dirty, or damaged optics, or due to variations in the laser gain medium itself. This filtering can be applied to transmit a pure transverse mode from a multimode laser while blocking other modes emitted from the optical resonator. The term "filtering" indicates that the desirable structural features of the original source pass through the filter, while the undesirable features are blocked. An apparatus which follows the filter effectively sees a higher-quality but lower-powered image of the source, instead of the actual source directly. An example of the use of spatial filter can be seen in advanced setup of micro-Raman spectroscopy. In spatial filtering, a lens is used to ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Connective Tissue
Connective tissue is one of the four primary types of animal tissue, along with epithelial tissue, muscle tissue, and nervous tissue. It develops from the mesenchyme derived from the mesoderm the middle embryonic germ layer. Connective tissue is found in between other tissues everywhere in the body, including the nervous system. The three meninges, membranes that envelop the brain and spinal cord are composed of connective tissue. Most types of connective tissue consists of three main components: elastic and collagen fibers, ground substance, and cells. Blood, and lymph are classed as specialized fluid connective tissues that do not contain fiber. All are immersed in the body water. The cells of connective tissue include fibroblasts, adipocytes, macrophages, mast cells and leucocytes. The term "connective tissue" (in German, ''Bindegewebe'') was introduced in 1830 by Johannes Peter Müller. The tissue was already recognized as a distinct class in the 18th century. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Voltage Sensitive Proteins
Voltage, also known as electric pressure, electric tension, or (electric) potential difference, is the difference in electric potential between two points. In a static electric field, it corresponds to the work needed per unit of charge to move a test charge between the two points. In the International System of Units, the derived unit for voltage is named ''volt''. The voltage between points can be caused by the build-up of electric charge (e.g., a capacitor), and from an electromotive force (e.g., electromagnetic induction in generator, inductors, and transformers). On a macroscopic scale, a potential difference can be caused by electrochemical processes (e.g., cells and batteries), the pressure-induced piezoelectric effect, and the thermoelectric effect. A voltmeter can be used to measure the voltage between two points in a system. Often a common reference potential such as the ground of the system is used as one of the points. A voltage can represent either a source ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescent Probes
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions). More generally they are covalently bonded to a macromolecule, serving as a marker (or dye, or tag, or reporter) for affine or bioactive reagents (antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, i.e., fluorescent imaging and spectroscopy. Fluorescein, via its amine-reactive isothiocyanate derivative fluorescein isothiocyanate (FITC), has been one of the most popular fluorophores. From an ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
T-tubule
T-tubules (transverse tubules) are extensions of the cell membrane that penetrate into the center of skeletal and cardiac muscle cells. With membranes that contain large concentrations of ion channels, transporters, and pumps, T-tubules permit rapid transmission of the action potential into the cell, and also play an important role in regulating cellular calcium concentration. Through these mechanisms, T-tubules allow heart muscle cells to contract more forcefully by synchronising calcium release from the sarcoplasmic reticulum throughout the cell. T-tubule structure and function are affected beat-by-beat by cardiomyocyte contraction, as well as by diseases, potentially contributing to heart failure and arrhythmias. Although these structures were first seen in 1897, research into T-tubule biology is ongoing. Structure T-tubules are tubules formed from the same Lipid bilayer, phospholipid bilayer as the surface membrane or sarcolemma of skeletal or cardiac muscle cells. They c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Response Time (technology)
In technology, response time is the time a system or functional unit takes to react to a given input. Computing Response time is the total amount of time it takes to respond to a request for service. That service can be anything from a memory fetch, to a disk IO, to a complex database query, or loading a full web page. Ignoring transmission time for a moment, the response time is the sum of the service time and wait time. The service time is the time it takes to do the work you requested. For a given request the service time varies little as the workload increases – to do X amount of work it always takes X amount of time. The wait time is how long the request had to wait in a queue before being serviced and it varies from zero, when no waiting is required, to a large multiple of the service time, as many requests are already in the queue and have to be serviced first. With basic queueing theory math you can calculate how the average wait time increases as the device providing ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
ANNINE-6plus
ANNINE-6plus is a water soluble voltage sensitive dye (also called potentiometric dyes). This compound was developed at the Max Planck Institute for Biochemistry in Germany. It is used to optically measure the changes in transmembrane voltage of excitable cells, including neurons, skeletal and cardiac myocytes. Voltage sensitivity ANNINE-6plus has a fractional fluorescent intensity change (Δ''F''/''F'' per 100 mV change) of about 30% with single-photon excitation (~488 nm) and >50% with two-photon excitation (~1060 nm). Applications ANNINE-6plus has been applied in the microscopic imaging of action potentials of cardiomyocyte in perfused mice heart. Using confocal microscopy in conjunction with ANNINE-6plus, single sweep action potentials with high peak signal-to-noise ratio (SNR) have been recorded from single transverse tubule ( t-tubule) of a few micrometers in the ventricular cardiomyocyte Cardiac muscle (also called heart muscle, myocardium, cardi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Physical Property
A physical property is any property that is measurable, whose value describes a state of a physical system. The changes in the physical properties of a system can be used to describe its changes between momentary states. Physical properties are often referred to as observables. They are not modal properties. A quantifiable physical property is called physical quantity. Physical properties are often characterized as intensive and extensive properties. An intensive property does not depend on the size or extent of the system, nor on the amount of matter in the object, while an extensive property shows an additive relationship. These classifications are in general only valid in cases when smaller subdivisions of the sample do not interact in some physical or chemical process when combined. Properties may also be classified with respect to the directionality of their nature. For example, isotropic properties do not change with the direction of observation, and anisotropic proper ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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