Phred Figure 1
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Phred Figure 1
Phred may refer to: *Phred (software), a computer program used in molecular biology *Phred quality score, a term used in molecular biology *Phred (Doonesbury), a character from the comic strip Doonesbury *Phred on Your Head Show, a children's television show *The URL with Phred Show, a spin-off of the above See also

*Fred (other) {{disambig ...
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Phred (software)
Phred is a computer program for base calling, that is to say, identifying a nucleobase sequence from fluorescence "trace" data generated by an automated DNA sequencer that uses electrophoresis and 4-fluorescent dye method. When originally developed, Phred produced significantly fewer errors in the data sets examined than other methods, averaging 40–50% fewer errors. Phred quality scores have become widely accepted to characterize the quality of DNA sequences, and can be used to compare the efficacy of different sequencing methods. Background The fluorescent-dye DNA sequencing is a molecular biology technique that involves labeling single-strand DNA sequences of varied length with 4 fluorescent dyes (corresponding to 4 different Nucleobase, bases used in DNA) and subsequently separating the DNA sequences by "slab gel"- or capillary-electrophoresis method (see DNA Sequencing). The electrophoresis run is monitored by a Charge-coupled device, CCD on the DNA sequencer and this produc ...
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Phred Quality Score
A Phred quality score is a measure of the quality of the identification of the nucleobases generated by automated DNA sequencing. It was originally developed for the computer program Phred (software), Phred to help in the automation of DNA sequencing in the Human Genome Project. Phred quality scores are assigned to each nucleotide base call in automated sequencer traces. The FASTQ format encodes phred scores as ASCII characters alongside the read sequences. Phred quality scores have become widely accepted to characterize the quality of DNA sequences, and can be used to compare the efficacy of different sequencing methods. Perhaps the most important use of Phred quality scores is the automatic determination of accurate, quality-based consensus sequences. Definition Phred quality scores Q are logarithmically related to the base-calling error probabilities P and defined as Q = -10 \ \log_ P. This relation can be also be written as P = 10^. For example, if Phred assigns a quali ...
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Phred (Doonesbury)
The comic strip ''Doonesbury'', by Garry Trudeau, features an extensive cast of characters with complex interpersonal relationships; as of 2018, the strip's official website lists twenty-four primary characters, with dozens more having been featured over the years, including some who were phased out of the strip only to be reintroduced years later. Kim Rosenthal, for example, first appeared as a recurring child character in the 1970s, then as a teenager in the 1980s, and was reintroduced as an adult in the 1990s. Main characters * Mike Doonesbury (October 26, 1970) – Former Walden College student, former roommate of B.D., and former Walden commune member, ex-advertising man and then co-founder of a software start-up; ex-husband of J.J., husband of Kim, and father of Alex. Originally from Tulsa, Oklahoma. * B.D. (October 26, 1970) – husband of Boopsie. A reservist and veteran of Vietnam and both Gulf Wars, he lost a leg in Iraq. Known for his conservative views and (until Ap ...
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Phred On Your Head Show
''Phred on Your Head Show'' is an American children's television series produced for Noggin, a cable channel co-founded by Nickelodeon and Sesame Workshop. The first episode aired on Nickelodeon as a sneak peek on June 6, 1999. Noggin aired encore showings of the first episode throughout June and started airing the show regularly on July 26, 1999. The show stars a small green character named Phred, voiced by Doug Preis, who has his own variety show. In each episode, Phred hops across different people's heads to find a host, who then chooses a selection of Noggin programs to play. Phred was animated by two studios: MTV Animation and Possible Worlds. The show was linked with Noggin's website, Noggin.com, and Phred's home was said to be inside the "dot" of Noggin.com. The episodes encouraged viewers to visit Noggin.com and send in their own ideas, comments, and questions for future episodes. These were integrated into the show as part of a segment called "Phred's Got Mail." Other se ...
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The URL With Phred Show
''The URL with Phred Show'' is an American children's television series, produced by Nickelodeon for its sister channel Noggin. It is a spin-off series to ''Phred on Your Head Show'', an earlier Noggin series with the same animated host: a small, green character named Phred. Premieres of ''The URL with Phred Show'' aired from September 10, 2001 until 2002, and reruns aired until 2004. The show is presented as an art showcase, and each episode follows Phred as he presents a mix of viewer-submitted artwork and messages from Noggin's website. The show was named after URL, a term for an online web address. In the episodes, the URL name is said to also stand for "U R Live," emphasizing how viewers could submit their own art and messages to Noggin's website to see them live on the show. In a departure from the original ''Phred on Your Head Show'', the spin-off is themed around a website interface, and there are a variety of new characters who co-host the show with Phred. Most of the new ...
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