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Purnell Equation
The Purnell equation is an equation used in analytical chemistry to calculate the resolution ''R''s between two peaks in a chromatogram. :R_s = \frac \left ( \frac \right ) \left ( \frac \right ) where ::''R''s is the resolution between the two peaks ::''N''2 is the plate number of the second peak ::''α'' is the separation factor between the two peaks ::''k'' '2 is the retention factor In chromatography, the retardation factor (''R'') is the fraction of an analyte in the mobile phase of a chromatographic system. In planar chromatography in particular, the retardation factor ''R''F is defined as the ratio of the distance traveled b ... of the second peak. The higher the resolution, the better the separation. References Chromatography Equations {{Chromatography-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Equation
In mathematics, an equation is a formula that expresses the equality of two expressions, by connecting them with the equals sign . The word ''equation'' and its cognates in other languages may have subtly different meanings; for example, in French an ''équation'' is defined as containing one or more variables, while in English, any well-formed formula consisting of two expressions related with an equals sign is an equation. ''Solving'' an equation containing variables consists of determining which values of the variables make the equality true. The variables for which the equation has to be solved are also called unknowns, and the values of the unknowns that satisfy the equality are called solutions of the equation. There are two kinds of equations: identities and conditional equations. An identity is true for all values of the variables. A conditional equation is only true for particular values of the variables. An equation is written as two expressions, connected by a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Analytical Chemistry
Analytical chemistry studies and uses instruments and methods to separate, identify, and quantify matter. In practice, separation, identification or quantification may constitute the entire analysis or be combined with another method. Separation isolates analytes. Qualitative analysis identifies analytes, while quantitative analysis determines the numerical amount or concentration. Analytical chemistry consists of classical, wet chemical methods and modern, instrumental methods. Classical qualitative methods use separations such as precipitation, extraction, and distillation. Identification may be based on differences in color, odor, melting point, boiling point, solubility, radioactivity or reactivity. Classical quantitative analysis uses mass or volume changes to quantify amount. Instrumental methods may be used to separate samples using chromatography, electrophoresis or field flow fractionation. Then qualitative and quantitative analysis can be performed, often with t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chromatogram
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the ''stationary phase'' is fixed. Because the different constituents of the mixture tend to have different affinities for the stationary phase and are retained for different lengths of time depending on their interactions with its surface sites, the constituents travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and the stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation. Chromatography may be preparative or analytical. The purpose of preparativ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Theoretical Plate
A theoretical plate in many separation processes is a hypothetical zone or stage in which two phases, such as the liquid and vapor phases of a substance, establish an equilibrium with each other. Such equilibrium stages may also be referred to as an equilibrium stage, ideal stage, or a theoretical tray. The performance of many separation processes depends on having series of equilibrium stages and is enhanced by providing more such stages. In other words, having more theoretical plates increases the efficiency of the separation process be it either a distillation, absorption, chromatographic, adsorption or similar process. Applications The concept of theoretical plates and trays or equilibrium stages is used in the design of many different types of separation. Distillation columns The concept of theoretical plates in designing distillation processes has been discussed in many reference texts. Any physical device that provides good contact between the vapor and liquid phases prese ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Retention Factor
In chromatography, the retardation factor (''R'') is the fraction of an analyte in the mobile phase of a chromatographic system. In planar chromatography in particular, the retardation factor ''R''F is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front. Ideally, the values for ''RF'' are equivalent to the R values used in column chromatography. Although the term retention factor is sometimes used synonymously with retardation factor in regard to planar chromatography the term is not defined in this context. However, in column chromatography, the retention factor or capacity factor (''k'') is defined as the ratio of time an analyte is retained in the stationary phase to the time it is retained in the mobile phase, which is inversely proportional to the retardation factor. General definition In chromatography, the retardation factor, ''R'', is the fraction of the sample in the mobile phase at equilibrium, defined as: ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chromatography
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the ''stationary phase'' is fixed. Because the different constituents of the mixture tend to have different affinities for the stationary phase and are retained for different lengths of time depending on their interactions with its surface sites, the constituents travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and the stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation. Chromatography may be preparative or analytical. The purpose of preparativ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
