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Pollen DNA Barcoding
Pollen DNA barcoding is the process of identifying pollen donor plant species through the amplification and sequencing of specific, conserved regions of plant DNA. Being able to accurately identify pollen has a wide range of applications though it has been difficult in the past due to the limitations of microscopic identification of pollen. Pollen identified using DNA barcoding involves the specific targeting of gene regions that are found in most to all plant species but have high variation between members of different species. The unique sequence of base pairs for each species within these target regions can be used as an identifying feature. The applications of pollen DNA barcoding range from forensics, to food safety, to conservation. Each of these fields benefits from the creation of plant barcode reference libraries. These libraries range largely in size and scope of their collections as well as what target region(s) they specialize in. One of the main challenges of ident ...
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Ligularia Pollen
''Ligularia'' (leopard plant) is a genus of Old World herbaceous plant, herbaceous perennial plants in the Senecioneae, groundsel tribe within the Asteraceae, sunflower family. They have yellow or orange pseudanthium, composite flower heads with brown or yellow central disc florets, and are native plant, native to damp habitats mostly in central and eastern Asia, with a few species from Europe. There are about 120Liu, J. Q., et al. (2006)Radiation and diversification within the ''Ligularia''–''Cremanthodium''–''Parasenecio'' complex (Asteraceae) triggered by uplift of the Qinghai-Tibetan Plateau.''Molecular Phylogenetics and Evolution'' 38(1) 31-49. to 140 species in the genus, and over half are endemism, endemic to China.Flora of China Vol. 20-21 Page 37 ...
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Herbarium
A herbarium (plural: herbaria) is a collection of preserved plant specimens and associated data used for scientific study. The specimens may be whole plants or plant parts; these will usually be in dried form mounted on a sheet of paper (called ''exsiccatum'', plur. ''exsiccata'') but, depending upon the material, may also be stored in boxes or kept in alcohol or other preservative. The specimens in a herbarium are often used as reference material in describing plant taxa; some specimens may be types. The same term is often used in mycology to describe an equivalent collection of preserved fungi, otherwise known as a fungarium. A xylarium is a herbarium specialising in specimens of wood. The term hortorium (as in the Liberty Hyde Bailey Hortorium) has occasionally been applied to a herbarium specialising in preserving material of horticultural origin. History The making of herbaria is an ancient phenomenon, at least six centuries old, although the techniques have changed l ...
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Molecular Genetics
Molecular genetics is a sub-field of biology that addresses how differences in the structures or expression of DNA molecules manifests as variation among organisms. Molecular genetics often applies an "investigative approach" to determine the structure and/or function of genes in an organism's genome using genetic screens.  The field of study is based on the merging of several sub-fields in biology: classical Mendelian inheritance, Cell biology, cellular biology, molecular biology, biochemistry, and biotechnology. Researchers search for mutations in a gene or induce mutations in a gene to link a gene sequence to a specific phenotype. Molecular genetics is a powerful methodology for linking mutations to genetic conditions that may aid the search for treatments/cures for various genetics diseases. History For molecular genetics to develop as a discipline, several scientific discoveries were necessary.  The discovery of DNA as a means to transfer the genetic code of life f ...
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Bioinformatics
Bioinformatics () is an interdisciplinary field that develops methods and software tools for understanding biological data, in particular when the data sets are large and complex. As an interdisciplinary field of science, bioinformatics combines biology, chemistry, physics, computer science, information engineering, mathematics and statistics to analyze and interpret the biological data. Bioinformatics has been used for '' in silico'' analyses of biological queries using computational and statistical techniques. Bioinformatics includes biological studies that use computer programming as part of their methodology, as well as specific analysis "pipelines" that are repeatedly used, particularly in the field of genomics. Common uses of bioinformatics include the identification of candidates genes and single nucleotide polymorphisms (SNPs). Often, such identification is made with the aim to better understand the genetic basis of disease, unique adaptations, desirable properties (e ...
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Aeroplankton
Aeroplankton (or aerial plankton) are tiny lifeforms that float and drift in the air, carried by wind. Most of the living things that make up aeroplankton are very small to microscopic in size, and many can be difficult to identify because of their tiny size. Scientists collect them for study in traps and sweep nets from aircraft, kites or balloons. Aeroplankton is made up mostly of microorganisms, including viruses, about 1,000 different species of bacteria, around 40,000 varieties of fungi, and hundreds of species of protists, algae, mosses, and liverworts that live some part of their life cycle as aeroplankton, often as spores, pollen, and wind-scattered seeds. Additionally, microorganisms are swept into the air from terrestrial dust storms, and an even larger amount of airborne marine microorganisms are propelled high into the atmosphere in sea spray. Aeroplankton deposits hundreds of millions of airborne viruses and tens of millions of bacteria every day on every square me ...
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DNA Metabarcoding
Metabarcoding is the DNA barcoding, barcoding of DNA/RNA (or Environmental DNA, eDNA/Environmental DNA, eRNA) in a manner that allows for the simultaneous identification of many taxa within the same sample. The main difference between barcoding and metabarcoding is that metabarcoding does not focus on one specific organism, but instead aims to determine species composition within a sample. A barcode consists of a short variable gene region (for example, see Algae DNA barcoding#Target regions, different markers/barcodes) which is useful for taxonomic assignment flanked by highly conserved gene regions which can be used for Primer (molecular biology), primer design. This idea of general barcoding originated in 2003 from researchers at the University of Guelph. The metabarcoding procedure, like general barcoding, proceeds in order through stages of DNA extraction, Polymerase chain reaction, PCR amplification, sequencing and data analysis. Different genes are used depending if th ...
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Pollen Basket
The pollen basket or corbicula (plural corbiculae) is part of the tibia on the hind legs of certain species of bees. They use the structure in harvesting pollen and carrying it to the nest or hive. Other species of bees have scopae instead. Etymology There was little formal description of the corbicula before Carl Linnaeus explained the biological function of pollen in the mid-18th century. In English the first edition of ''Encyclopædia Britannica'' described the structure in 1771 without giving it any special name. The second edition, 1777, refers to the corbicula simply as the "basket". By 1802 William Kirby had introduced the New Latin term into English. He had borrowed it, with acknowledgement, from Réaumur. Like other Latin anatomical terms, this had the advantages of specificity, international acceptability, and culture neutrality. By 1820 the term ''pollen-basket'' seems to have gained acceptance in beekeeping, extracted in though a century later a compendium of e ...
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Cannabis (drug)
Cannabis, also known as marijuana among List of names for cannabis, other names, is a psychoactive drug from the cannabis plant. Native to Central or South Asia, the cannabis plant has been used as a drug for both Recreational marijuana, recreational and Entheogenic use of cannabis, entheogenic purposes and in various traditional medicines for centuries. Tetrahydrocannabinol (THC) is the main psychoactive component of cannabis, which is one of the 483 known compounds in the plant, including at least 65 other cannabinoids, such as cannabidiol (CBD). Cannabis can be used by Cannabis smoking, smoking, Vaporizer (inhalation device), vaporizing, Cannabis edible, within food, or Tincture of cannabis, as an extract. Cannabis has various effects of cannabis, mental and physical effects, which include euphoria, altered states of mind and Cannabis and time perception, sense of time, difficulty concentrating, Cannabis and memory, impaired short-term memory, impaired motor skill, body mo ...
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Conservation Movement
The conservation movement, also known as nature conservation, is a political, environmental, and social movement that seeks to manage and protect natural resources, including animal, fungus, and plant species as well as their habitat for the future. Conservationists are concerned with leaving the environment in a better state than the condition they found it in. Evidence-based conservation seeks to use high quality scientific evidence to make conservation efforts more effective. The early conservation movement evolved out of necessity to maintain natural resources such as fisheries, wildlife management, water, soil, as well as conservation and sustainable forestry. The contemporary conservation movement has broadened from the early movement's emphasis on use of sustainable yield of natural resources and preservation of wilderness areas to include preservation of biodiversity. Some say the conservation movement is part of the broader and more far-reaching environmental movem ...
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Pollination Network
A pollination network is a bipartite mutualistic network in which plants and pollinators are the nodes, and the pollination interactions form the links between these nodes. The pollination network is bipartite as interactions only exist between two distinct, non-overlapping sets of species, but not within the set: a pollinator can never be pollinated, unlike in a predator-prey network where a predator can be depredated. A pollination network is two-modal, i.e., it includes only links connecting plant and animal communities. Nested structure of pollination networks A key feature of pollination networks is their nested design. A study of 52 mutualist networks (including plant-pollinator interactions and plant-seed disperser interactions) found that most of the networks were nested. This means that the core of the network is made up of highly connected generalists (a pollinator that visits many different species of plant), while specialized species interact with a subset of the ...
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Butterfly With Flower
Butterflies are insects in the macrolepidopteran clade Rhopalocera from the order Lepidoptera, which also includes moths. Adult butterflies have large, often brightly coloured wings, and conspicuous, fluttering flight. The group comprises the large superfamily Papilionoidea, which contains at least one former group, the skippers (formerly the superfamily "Hesperioidea"), and the most recent analyses suggest it also contains the moth-butterflies (formerly the superfamily "Hedyloidea"). Butterfly fossils date to the Paleocene, about 56 million years ago. Butterflies have a four-stage life cycle, as like most insects they undergo complete metamorphosis. Winged adults lay eggs on the food plant on which their larvae, known as caterpillars, will feed. The caterpillars grow, sometimes very rapidly, and when fully developed, pupate in a chrysalis. When metamorphosis is complete, the pupal skin splits, the adult insect climbs out, and after its wings have expanded and dried, it fli ...
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Taxonomic Resolution
DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise of DNA barcoding is that by comparison with a reference library of such DNA sections (also called "DNA sequence, sequences"), an individual sequence can be used to uniquely identify an organism to species, just as a supermarket scanner uses the familiar black stripes of the Universal Product Code, UPC barcode to identify an item in its stock against its reference database. These "barcodes" are sometimes used in an effort to identify unknown species or parts of an organism, simply to catalog as many Taxon, taxa as possible, or to compare with traditional taxonomy in an effort to determine species boundaries. Different gene regions are used to identify the different organismal groups using barcoding. The most commonly used barcode region for animals and some protists is a portion of the Cytochrome c oxidase subunit I, cytochrome ''c'' oxidase I (COI or Cytochr ...
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