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Optical Unit
Optical units are dimensionless units of length used in optical microscopy. They are used to express distances in terms of the numerical aperture of the system and the wavelength of the light used for observation. Using these units allows comparison of the properties of different microscopes. For example, the diameter of the first minimum of the Airy disk is always 7.6 optical units in the image plane of a diffraction-limited system, diffraction limited microscope. Equations There are two types of optical units. ''Radial optical units'' are measured in the image plane, and ''axial optical units'' are used to measure distances between the image plane and the observer. The number of optical units v in a given radial length r is given by: v_\mathrm = \frac \frac r where: * \lambda is the wavelength * n \sin \alpha is the numerical aperture * M_\mathrm is the total magnification Axial optical units are more complicated, as there is no simple definition of resolution in the ax ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dimensionless Unit
Dimensionless quantities, or quantities of dimension one, are quantities implicitly defined in a manner that prevents their aggregation into units of measurement. ISBN 978-92-822-2272-0. Typically expressed as ratios that align with another system, these quantities do not necessitate explicitly defined units. For instance, alcohol by volume (ABV) represents a volumetric ratio; its value remains independent of the specific units of volume used, such as in milliliters per milliliter (mL/mL). The number one is recognized as a dimensionless base quantity. Radians serve as dimensionless units for angular measurements, derived from the universal ratio of 2π times the radius of a circle being equal to its circumference. Dimensionless quantities play a crucial role serving as parameters in differential equations in various technical disciplines. In calculus, concepts like the unitless ratios in limits or derivatives often involve dimensionless quantities. In differential geom ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microscopy
Microscopy is the technical field of using microscopes to view subjects too small to be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical microscope, optical, electron microscope, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection (physics), reflection, or refraction of electromagnetic radiation/electron beams interacting with the Laboratory specimen, specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscope, transmission electron microscopy) or by scanning a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scan ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Numerical Aperture
In optics, the numerical aperture (NA) of an optical system is a dimensionless number that characterizes the range of angles over which the system can accept or emit light. By incorporating index of refraction in its definition, has the property that it is constant for a beam as it goes from one material to another, provided there is no refractive power at the interface (e.g., a flat interface). The exact definition of the term varies slightly between different areas of optics. Numerical aperture is commonly used in microscopy to describe the acceptance cone of an Objective (optics), objective (and hence its light-gathering ability and Optical resolution, resolution), and in fiber optics, in which it describes the range of angles within which light that is incident on the fiber will be transmitted along it. General optics In most areas of optics, and especially in microscopy, the numerical aperture of an optical system such as an objective lens is defined by \mathrm = n \sin \t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Wavelength
In physics and mathematics, wavelength or spatial period of a wave or periodic function is the distance over which the wave's shape repeats. In other words, it is the distance between consecutive corresponding points of the same ''phase (waves), phase'' on the wave, such as two adjacent crests, troughs, or zero crossings. Wavelength is a characteristic of both traveling waves and standing waves, as well as other spatial wave patterns. The multiplicative inverse, inverse of the wavelength is called the ''spatial frequency''. Wavelength is commonly designated by the Greek letter lambda (''λ''). For a modulated wave, ''wavelength'' may refer to the carrier wavelength of the signal. The term ''wavelength'' may also apply to the repeating envelope (mathematics), envelope of modulated waves or waves formed by Interference (wave propagation), interference of several sinusoids. Assuming a sinusoidal wave moving at a fixed phase velocity, wave speed, wavelength is inversely proportion ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Airy Disk
In optics, the Airy disk (or Airy disc) and Airy pattern are descriptions of the best-focus (optics), focused Point source#Light, spot of light that a perfect lens (optics), lens with a circular aperture can make, limited by the diffraction of light. The Airy disk is of importance in physics, optics, and astronomy. The diffraction pattern resulting from a uniformly illuminated, circular aperture has a bright circle of confusion, central region, known as the Airy disk, which together with the series of concentric objects, concentric rings around is called the Airy pattern. Both are named after George Biddell Airy. The disk and rings phenomenon had been known prior to Airy; John Herschel described the appearance of a bright star seen through a telescope under high magnification for an 1828 article on light for the ''Encyclopedia Metropolitana'': Airy wrote the first full theoretical treatment explaining the phenomenon (his 1835 "On the Diffraction of an Object-glass with Circul ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Image Plane
In 3D computer graphics, the image plane is that plane in the world which is identified with the plane of the display monitor used to view the image that is being rendered. It is also referred to as screen space. If one makes the analogy of taking a photograph to rendering a 3D image, the surface of the film is the image plane. In this case, the viewing transformation is a projection that maps the world onto the image plane. A rectangular region of this plane, called the viewing window or viewport, maps to the monitor. This establishes the mapping between pixels on the monitor and points (or rather, rays) in the 3D world. The plane is not usually an actual geometric object in a 3D scene, but instead is usually a collection of target coordinates or dimensions that are used during the rasterization process so the final output can be displayed as intended on the physical screen. In optics, the image plane is the plane that contains the object's projected image, and lies beyond th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Diffraction-limited System
In optics, any optical instrument or systema microscope, telescope, or camerahas a principal limit to its resolution due to the physics of diffraction. An optical instrument is said to be diffraction-limited if it has reached this limit of resolution performance. Other factors may affect an optical system's performance, such as lens imperfections or aberrations, but these are caused by errors in the manufacture or calculation of a lens, whereas the diffraction limit is the maximum resolution possible for a theoretically perfect, or ideal, optical system. The diffraction-limited angular resolution, in radians, of an instrument is proportional to the wavelength of the light being observed, and inversely proportional to the diameter of its objective's entrance aperture. For telescopes with circular apertures, the size of the smallest feature in an image that is diffraction limited is the size of the Airy disk. As one decreases the size of the aperture of a telescopic lens, diff ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Magnification
Magnification is the process of enlarging the apparent size, not physical size, of something. This enlargement is quantified by a size ratio called optical magnification. When this number is less than one, it refers to a reduction in size, sometimes called ''de-magnification''. Typically, magnification is related to scaling up visuals or images to be able to see more detail, increasing resolution, using microscope, printing techniques, or digital processing. In all cases, the magnification of the image does not change the perspective of the image. Examples of magnification Some optical instruments provide visual aid by magnifying small or distant subjects. * A magnifying glass, which uses a positive (convex) lens to make things look bigger by allowing the user to hold them closer to their eye. * A telescope, which uses its large objective lens or primary mirror to create an image of a distant object and then allows the user to examine the image closely with a smaller ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microscopy
Microscopy is the technical field of using microscopes to view subjects too small to be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical microscope, optical, electron microscope, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection (physics), reflection, or refraction of electromagnetic radiation/electron beams interacting with the Laboratory specimen, specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscope, transmission electron microscopy) or by scanning a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scan ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
