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Nitrogen-15 Tracing
Nitrogen-15 (15N) tracing is a technique to study the nitrogen cycle using the heavier, stable nitrogen isotope 15 N. Despite the different weights, 15N is involved in the same chemical reactions as the more abundant 14N and is therefore used to trace and quantify conversions of one nitrogen compound to another. 15N tracing is applied in biogeochemistry, soil science, environmental science, environmental microbiology and small molecule activation research. Applications 15N tracing allows researchers to distinguish specific nitrogen conversions from a network of simultaneous reactions; e.g. ammonium can at the same time be oxidised by autotrophic microorganisms, produced by mineralisation of organic matter, produced by dissimilatory nitrate reduction and assimilated by microbes and plants. In this case, quantifying the absolute amounts of ammonium does not explain how it is produced or consumed. However, the conversion of one 15N labelled compound to another can directly be linke ...
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Nitrogen Cycle
The nitrogen cycle is the biogeochemical cycle by which nitrogen is converted into multiple chemical forms as it circulates among atmospheric, terrestrial, and marine ecosystems. The conversion of nitrogen can be carried out through both biological and physical processes. Important processes in the nitrogen cycle include fixation, ammonification, nitrification, and denitrification. The majority of Earth's atmosphere (78%) is atmospheric nitrogen, making it the largest source of nitrogen. However, atmospheric nitrogen has limited availability for biological use, leading to a scarcity of usable nitrogen in many types of ecosystems. The nitrogen cycle is of particular interest to ecologists because nitrogen availability can affect the rate of key ecosystem processes, including primary production and decomposition. Human activities such as fossil fuel combustion, use of artificial nitrogen fertilizers, and release of nitrogen in wastewater have dramatically altered the global nitr ...
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Nitrogen
Nitrogen is the chemical element with the symbol N and atomic number 7. Nitrogen is a nonmetal and the lightest member of group 15 of the periodic table, often called the pnictogens. It is a common element in the universe, estimated at seventh in total abundance in the Milky Way and the Solar System. At standard temperature and pressure, two atoms of the element bond to form N2, a colorless and odorless diatomic gas. N2 forms about 78% of Earth's atmosphere, making it the most abundant uncombined element. Nitrogen occurs in all organisms, primarily in amino acids (and thus proteins), in the nucleic acids ( DNA and RNA) and in the energy transfer molecule adenosine triphosphate. The human body contains about 3% nitrogen by mass, the fourth most abundant element in the body after oxygen, carbon, and hydrogen. The nitrogen cycle describes the movement of the element from the air, into the biosphere and organic compounds, then back into the atmosphere. Many indus ...
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Isotope
Isotopes are two or more types of atoms that have the same atomic number (number of protons in their nuclei) and position in the periodic table (and hence belong to the same chemical element), and that differ in nucleon numbers (mass numbers) due to different numbers of neutrons in their nuclei. While all isotopes of a given element have almost the same chemical properties, they have different atomic masses and physical properties. The term isotope is formed from the Greek roots isos ( ἴσος "equal") and topos ( τόπος "place"), meaning "the same place"; thus, the meaning behind the name is that different isotopes of a single element occupy the same position on the periodic table. It was coined by Scottish doctor and writer Margaret Todd in 1913 in a suggestion to the British chemist Frederick Soddy. The number of protons within the atom's nucleus is called its atomic number and is equal to the number of electrons in the neutral (non-ionized) atom. Each atomic numbe ...
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Nitrogen-15
Natural nitrogen (7N) consists of two stable isotopes: the vast majority (99.6%) of naturally occurring nitrogen is nitrogen-14, with the remainder being nitrogen-15. Fourteen radioisotopes are also known, with atomic masses ranging from 10 to 25, along with one nuclear isomer, 11mN. All of these radioisotopes are short-lived, the longest-lived being nitrogen-13 with a half-life of . All of the others have half-lives below 7.15 seconds, with most of these being below 620 milliseconds. Most of the isotopes with atomic mass numbers below 14 decay to isotopes of carbon, while most of the isotopes with masses above 15 decay to isotopes of oxygen. The shortest-lived known isotope is nitrogen-10, with a half-life of . List of isotopes , - , , style="text-align:right" , 7 , style="text-align:right" , 3 , , , p ?Decay mode shown is energetically allowed, but has not been experimentally observed to occur in this nuclide. , ? , 1−, 2− , , , - , , style="text-align:r ...
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Biogeochemistry
Biogeochemistry is the scientific discipline that involves the study of the chemical, physical, geological, and biological processes and reactions that govern the composition of the natural environment (including the biosphere, the cryosphere, the hydrosphere, the pedosphere, the atmosphere, and the lithosphere). In particular, biogeochemistry is the study of biogeochemical cycles, the cycles of chemical elements such as carbon and nitrogen, and their interactions with and incorporation into living things transported through earth scale biological systems in space and time. The field focuses on chemical cycles which are either driven by or influence biological activity. Particular emphasis is placed on the study of carbon, nitrogen, sulfur, iron, and phosphorus cycles. Biogeochemistry is a systems science closely related to systems ecology. History Early History Early Greeks established some of the core ideas of biogeochemistry, such as nature consisting of cycles. 17th ...
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Soil Science
Soil science is the study of soil as a natural resource on the surface of the Earth including soil formation, classification and mapping; physical, chemical, biological, and fertility properties of soils; and these properties in relation to the use and management of soils.Jackson, J. A. (1997). Glossary of Geology (4. ed.). Alexandria, Virginia: American Geological Institute. p 604. Sometimes terms which refer to branches of soil science, such as pedology (formation, chemistry, morphology, and classification of soil) and edaphology (how soils interact with living things, especially plants), are used as if synonymous with soil science. The diversity of names associated with this discipline is related to the various associations concerned. Indeed, engineers, agronomists, chemists, geologists, physical geographers, ecologists, biologists, microbiologists, silviculturists, sanitarians, archaeologists, and specialists in regional planning, all contribute to further knowledge ...
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Environmental Science
Environmental science is an interdisciplinary academic field that integrates physics, biology, and geography (including ecology, chemistry, plant science, zoology, mineralogy, oceanography, limnology, soil science, geology and physical geography, and atmospheric science) to the study of the environment, and the solution of environmental problems. Environmental science emerged from the fields of natural history and medicine during the Enlightenment. Today it provides an integrated, quantitative, and interdisciplinary approach to the study of environmental systems. Environmental studies incorporates more of the social sciences for understanding human relationships, perceptions and policies towards the environment. Environmental engineering focuses on design and technology for improving environmental quality in every aspect. Environmental scientists seek to understand the earth’s physical, chemical, biological, and geological processes, and to use that knowledge to understan ...
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Environmental Microbiology
A biophysical environment is a biotic and abiotic surrounding of an organism or population, and consequently includes the factors that have an influence in their survival, development, and evolution. A biophysical environment can vary in scale from microscopic to global in extent. It can also be subdivided according to its attributes. Examples include the marine environment, the atmospheric environment and the terrestrial environment. The number of biophysical environments is countless, given that each living organism has its own environment. The term ''environment'' can refer to a singular global environment in relation to humanity, or a local biophysical environment, e.g. the UK's Environment Agency. Life-environment interaction All life that has survived must have adapted to the conditions of its environment. Temperature, light, humidity, soil nutrients, etc., all influence the species within an environment. However, life in turn modifies, in various forms, its conditions. S ...
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Ammonium
The ammonium cation is a positively-charged polyatomic ion with the chemical formula or . It is formed by the protonation of ammonia (). Ammonium is also a general name for positively charged or protonated substituted amines and quaternary ammonium cations (), where one or more hydrogen atoms are replaced by organic groups (indicated by R). Acid–base properties The ammonium ion is generated when ammonia, a weak base, reacts with Brønsted acids (proton donors): :H+ + NH3 -> H4 The ammonium ion is mildly acidic, reacting with Brønsted bases to return to the uncharged ammonia molecule: : H4 + B- -> HB + NH3 Thus, treatment of concentrated solutions of ammonium salts with strong base gives ammonia. When ammonia is dissolved in water, a tiny amount of it converts to ammonium ions: :H2O + NH3 OH- + H4 The degree to which ammonia forms the ammonium ion depends on the pH of the solution. If the pH is low, the equilibrium shifts to the right: more ammonia molecules are co ...
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Nitrification
''Nitrification'' is the biological oxidation of ammonia to nitrite followed by the oxidation of the nitrite to nitrate occurring through separate organisms or direct ammonia oxidation to nitrate in comammox bacteria. The transformation of ammonia to nitrite is usually the rate limiting step of nitrification. Nitrification is an important step in the nitrogen cycle in soil. Nitrification is an aerobic process performed by small groups of autotrophic bacteria and archaea. Microbiology Ammonia oxidation The oxidation of ammonia into nitrite (also known as nitritation) is performed by two groups of organisms, ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA). AOB AOB can be found among the Betaproteobacteria and Gammaproteobacteria. Since discovery of AOA in 2005, two isolates have been cultivated: ''Nitrosopumilus maritimus'' and ''Nitrososphaera viennensis''. In soils the most studied AOB belong to the genera ''Nitrosomonas'' and '' Nitrococcus''. AOA ...
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Soil
Soil, also commonly referred to as earth or dirt, is a mixture of organic matter, minerals, gases, liquids, and organisms that together support life. Some scientific definitions distinguish ''dirt'' from ''soil'' by restricting the former term specifically to displaced soil. Soil consists of a solid phase of minerals and organic matter (the soil matrix), as well as a porous phase that holds gases (the soil atmosphere) and water (the soil solution). Accordingly, soil is a three-state system of solids, liquids, and gases. Soil is a product of several factors: the influence of climate, relief (elevation, orientation, and slope of terrain), organisms, and the soil's parent materials (original minerals) interacting over time. It continually undergoes development by way of numerous physical, chemical and biological processes, which include weathering with associated erosion. Given its complexity and strong internal connectedness, soil ecologists regard soil as an ecosystem. Most ...
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Nitrous Oxide
Nitrous oxide (dinitrogen oxide or dinitrogen monoxide), commonly known as laughing gas, nitrous, or nos, is a chemical compound, an oxide of nitrogen with the formula . At room temperature, it is a colourless non-flammable gas, and has a slightly sweet scent and taste. At elevated temperatures, nitrous oxide is a powerful oxidiser similar to molecular oxygen. Nitrous oxide has significant medical uses, especially in surgery and dentistry, for its anaesthetic and pain-reducing effects. Its colloquial name, "laughing gas", coined by Humphry Davy, is due to the euphoric effects upon inhaling it, a property that has led to its recreational use as a dissociative anaesthetic. It is on the World Health Organization's List of Essential Medicines. It is also used as an oxidiser in rocket propellants, and in motor racing to increase the power output of engines. Nitrous oxide's atmospheric concentration reached 333 parts per billion (ppb) in 2020, increasing at a rate of abo ...
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