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Multifocal Plane Microscopy
Multifocal plane microscopy (MUM) or multiplane microscopy or multifocus microscopy is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial resolution by simultaneously imaging different focal planes within the specimen. In this methodology, the light collected from the sample by an infinity-corrected objective lens is split into two paths. In each path the split light is focused onto a detector which is placed at a specific calibrated distance from the tube lens. In this way, each detector images a distinct plane within the sample. The first developed MUM setup was capable of imaging two distinct planes within the sample. However, the setup can be modified to image more than two planes by further splitting the light in each light path and focusing it onto detectors placed at specific calibrated distances. It has later been improved for imaging up to four distinct planes. To image a greater number of focal planes, simple ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Schematic Diagram Of A Multifocal Plane Microscope
A schematic, or schematic diagram, is a designed representation of the elements of a system using abstract, graphic symbols rather than realistic pictures. A schematic usually omits all details that are not relevant to the key information the schematic is intended to convey, and may include oversimplified elements in order to make this essential meaning easier to grasp, as well as additional organization of the information. For example, a subway map intended for passengers may represent a subway station with a dot. The dot is not intended to resemble the actual station at all but aims to give the viewer information without unnecessary visual clutter. A schematic diagram of a chemical process uses symbols in place of detailed representations of the vessels, piping, valves, pumps, and other equipment that compose the system, thus emphasizing the functions of the individual elements and the interconnections among them and suppresses their physical details. In an electronic circuit ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
CCD Camera
A charge-coupled device (CCD) is an integrated circuit containing an array of linked, or coupled, capacitors. Under the control of an external circuit, each capacitor can transfer its electric charge to a neighboring capacitor. CCD sensors are a major technology used in digital imaging. In a CCD image sensor, pixels are represented by p-doped metal–oxide–semiconductor (MOS) capacitors. These MOS capacitors, the basic building blocks of a CCD, are biased above the threshold for inversion when image acquisition begins, allowing the conversion of incoming photons into electron charges at the semiconductor-oxide interface; the CCD is then used to read out these charges. Although CCDs are not the only technology to allow for light detection, CCD image sensors are widely used in professional, medical, and scientific applications where high-quality image data are required. In applications with less exacting quality demands, such as consumer and professional digital cameras, a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence Techniques
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, than the absorbed radiation. A perceptible example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the electromagnetic spectrum (invisible to the human eye), while the emitted light is in the visible region; this gives the fluorescent substance a distinct color that can only be seen when the substance has been exposed to UV light. Fluorescent materials cease to glow nearly immediately when the radiation source stops, unlike phosphorescent materials, which continue to emit light for some time after. Fluorescence has many practical applications, including mineralogy, gemology, medicine, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, vacuum ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microscopy
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
UT Southwestern Medical Center
The University of Texas Southwestern Medical Center (UT Southwestern or UTSW) is a public academic health science center in Dallas, Texas. With approximately 18,800 employees, more than 2,900 full-time faculty, and nearly 4 million outpatient visits per year, UT Southwestern is the largest medical school in the University of Texas System and state of Texas.https://utsystem.edu/sites/default/files/documents/publication/2018/fast-facts/fast-facts-09-2018.pdf UT Southwestern's William P. Clements Jr University Hospital is nationally ranked in nine specialties by '' U.S. News & World Report'' and ranked the best hospital in the Dallas-Fort Worth/ North Texas region. Forbes ranked UT Southwestern Medical Center as the top health care employer in the state of Texas and the top health care employer to new graduates in the United States. UT Southwestern's operating budget in 2021 was more than $4.1 billion, and is the largest medical institution in the Dallas–Fort Worth Metrop ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dual Objective MUM
Dual or Duals may refer to: Paired/two things * Dual (mathematics), a notion of paired concepts that mirror one another ** Dual (category theory), a formalization of mathematical duality *** see more cases in :Duality theories * Dual (grammatical number), a grammatical category used in some languages * Dual county, a Gaelic games county which in both Gaelic football and hurling * Dual diagnosis, a psychiatric diagnosis of co-occurrence of substance abuse and a mental problem * Dual fertilization, simultaneous application of a P-type and N-type fertilizer * Dual impedance, electrical circuits that are the dual of each other * Dual SIM cellphone supporting use of two SIMs * Aerochute International Dual a two-seat Australian powered parachute design Acronyms and other uses * Dual (brand), a manufacturer of Hifi equipment * DUAL (cognitive architecture), an artificial intelligence design model * DUAL algorithm, or diffusing update algorithm, used to update Internet protocol routing t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Single Particle Tracking
Single-particle tracking (SPT) is the observation of the motion of individual particles within a medium. The coordinates time series, which can be either in two dimensions (''x'', ''y'') or in three dimensions (''x'', ''y'', ''z''), is referred to as a ''trajectory''. The trajectory is typically analyzed using statistical methods to extract information about the underlying dynamics of the particle. These dynamics can reveal information about the type of transport being observed (e.g., thermal or active), the medium where the particle is moving, and interactions with other particles. In the case of random motion, trajectory analysis can be used to measure the diffusion coefficient. Applications In life sciences, single-particle tracking is broadly used to quantify the dynamics of molecules/proteins in live cells (of bacteria, yeast, mammalian cells and live ''Drosophila'' embryos). It has been extensively used to study the transcription factor dynamics in live cells. This method h ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Super-resolution Microscopy
Super-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit, which is due to the diffraction of light. Super-resolution imaging techniques rely on the near-field (photon-tunneling microscopy as well as those that utilize the Pendry Superlens and near field scanning optical microscopy) or on the far-field. Among techniques that rely on the latter are those that improve the resolution only modestly (up to about a factor of two) beyond the diffraction-limit, such as confocal microscopy with closed pinhole or aided by computational methods such as deconvolution or detector-based pixel reassignment (e.g. re-scan microscopy, pixel reassignment), the 4Pi microscope, and structured-illumination microscopy technologies such as SIM and SMI. There are two major groups of methods for super-resolution microscopy in the far-field that can improve the resolution by a much larger f ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Point Source
A point source is a single identifiable ''localised'' source of something. A point source has negligible extent, distinguishing it from other source geometries. Sources are called point sources because in mathematical modeling, these sources can usually be approximated as a mathematical point to simplify analysis. The actual source need not be physically small, if its size is negligible relative to other length scales in the problem. For example, in astronomy, stars are routinely treated as point sources, even though they are in actuality much larger than the Earth. In three dimensions, the density of something leaving a point source decreases in proportion to the inverse square of the distance from the source, if the distribution is isotropic, and there is no absorption or other loss. Mathematics In mathematics, a point source is a singularity from which flux or flow is emanating. Although singularities such as this do not exist in the observable universe, mathematical po ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Numerical Aperture
In optics, the numerical aperture (NA) of an optical system is a dimensionless number that characterizes the range of angles over which the system can accept or emit light. By incorporating index of refraction in its definition, NA has the property that it is constant for a beam as it goes from one material to another, provided there is no refractive power at the interface. The exact definition of the term varies slightly between different areas of optics. Numerical aperture is commonly used in microscopy to describe the acceptance cone of an objective (and hence its light-gathering ability and resolution), and in fiber optics, in which it describes the range of angles within which light that is incident on the fiber will be transmitted along it. General optics In most areas of optics, and especially in microscopy, the numerical aperture of an optical system such as an objective lens is defined by :\mathrm = n \sin \theta, where is the index of refraction of the medium in ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Depth Discrimination Of MUM Vs Single Plane
Depth(s) may refer to: Science and mathematics * Three-dimensional space * Depth (ring theory), an important invariant of rings and modules in commutative and homological algebra * Depth in a well, the measurement between two points in an oil well * Color depth (or "number of bits" or "bit depth"), in computer graphics * Market depth, in financial markets, the size of an order needed to move the market a given amount * Moulded depth, a nautical measurement * Sequence depth, or coverage, in genetic sequencing * Depth (coordinate), a type of vertical distance * Tree depth Art and entertainment * ''Depth'' (video game), an Indie video game * ''Depths'' (novel), a 2004 novel by Henning Mankell * ''Depths'' (Oceano album), 2009 * ''Depths'' (Windy & Carl album), 1998 * "Depths" (''Law & Order: Criminal Intent''), an episode of ''Law & Order: Criminal Intent'' * ''Depth'', the Japanese title for the PlayStation game released in Europe under the name '' Fluid'' * Depth, an ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Proceedings Of The National Academy Of Sciences
''Proceedings of the National Academy of Sciences of the United States of America'' (often abbreviated ''PNAS'' or ''PNAS USA'') is a peer-reviewed multidisciplinary scientific journal. It is the official journal of the National Academy of Sciences, published since 1915, and publishes original research, scientific reviews, commentaries, and letters. According to ''Journal Citation Reports'', the journal has a 2021 impact factor of 12.779. ''PNAS'' is the second most cited scientific journal, with more than 1.9 million cumulative citations from 2008 to 2018. In the mass media, ''PNAS'' has been described variously as "prestigious", "sedate", "renowned" and "high impact". ''PNAS'' is a delayed open access journal, with an embargo period of six months that can be bypassed for an author fee ( hybrid open access). Since September 2017, open access articles are published under a Creative Commons license. Since January 2019, ''PNAS'' has been online-only, although print issues are a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
