Multiangle Light Scattering
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Multiangle Light Scattering
Multiangle light scattering (MALS) describes a technique for measuring the light scattered by a sample into a plurality of angles. It is used for determining both the absolute molar mass and the average size of molecules in solution, by detecting how they scatter light. A collimated beam from a laser source is most often used, in which case the technique can be referred to as multiangle laser light scattering (MALLS). The insertion of the word ''laser'' was intended to reassure those used to making light scattering measurements with conventional light sources, such as Hg-arc lamps that low-angle measurements could now be made. Until the advent of lasers and their associated fine beams of narrow width, the width of conventional light beams used to make such measurements prevented data collection at smaller scattering angles. In recent years, since all commercial light scattering instrumentation use laser sources, this need to mention the light source has been dropped and the term ...
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Light
Light or visible light is electromagnetic radiation that can be perceived by the human eye. Visible light is usually defined as having wavelengths in the range of 400–700 nanometres (nm), corresponding to frequencies of 750–420 terahertz, between the infrared (with longer wavelengths) and the ultraviolet (with shorter wavelengths). In physics, the term "light" may refer more broadly to electromagnetic radiation of any wavelength, whether visible or not. In this sense, gamma rays, X-rays, microwaves and radio waves are also light. The primary properties of light are intensity, propagation direction, frequency or wavelength spectrum and polarization. Its speed in a vacuum, 299 792 458 metres a second (m/s), is one of the fundamental constants of nature. Like all types of electromagnetic radiation, visible light propagates by massless elementary particles called photons that represents the quanta of electromagnetic field, and can be analyzed as both waves and par ...
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Phytoplankton
Phytoplankton () are the autotrophic (self-feeding) components of the plankton community and a key part of ocean and freshwater ecosystems. The name comes from the Greek words (), meaning 'plant', and (), meaning 'wanderer' or 'drifter'. Phytoplankton obtain their energy through photosynthesis, as do trees and other plants on land. This means phytoplankton must have light from the sun, so they live in the well-lit surface layers (euphotic zone) of oceans and lakes. In comparison with terrestrial plants, phytoplankton are distributed over a larger surface area, are exposed to less seasonal variation and have markedly faster turnover rates than trees (days versus decades). As a result, phytoplankton respond rapidly on a global scale to climate variations. Phytoplankton form the base of marine and freshwater food webs and are key players in the global carbon cycle. They account for about half of global photosynthetic activity and at least half of the oxygen production, despite ...
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Solid Angle
In geometry, a solid angle (symbol: ) is a measure of the amount of the field of view from some particular point that a given object covers. That is, it is a measure of how large the object appears to an observer looking from that point. The point from which the object is viewed is called the ''apex'' of the solid angle, and the object is said to '' subtend'' its solid angle at that point. In the International System of Units (SI), a solid angle is expressed in a dimensionless unit called a ''steradian'' (symbol: sr). One steradian corresponds to one unit of area on the unit sphere surrounding the apex, so an object that blocks all rays from the apex would cover a number of steradians equal to the total surface area of the unit sphere, 4\pi. Solid angles can also be measured in squares of angular measures such as degrees, minutes, and seconds. A small object nearby may subtend the same solid angle as a larger object farther away. For example, although the Moon is much smaller ...
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Subtended Angle
In geometry, an angle is subtended by an arc, line segment or any other section of a curve when its two rays pass through the endpoints of that arc, line segment or curve section. Conversely, the arc, line segment or curve section confined within the rays of an angle is regarded as the corresponding subtension of that angle. We also sometimes say that an arc is intercepted or enclosed by that angle. The precise meaning varies with context. For example, one may speak of the angle subtended by an arc of a circle when the angle's vertex is the centre of the circle. See also * Central angle * Inscribed angle External links Definition of subtended angle mathisfun.com, with interactive applet In computing, an applet is any small application that performs one specific task that runs within the scope of a dedicated widget engine or a larger program, often as a plug-in. The term is frequently used to refer to a Java applet, a program w ... How an object subtends an angle '' ...
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Lorenz–Mie Scattering
The Mie solution to Maxwell's equations (also known as the Lorenz–Mie solution, the Lorenz–Mie–Debye solution or Mie scattering) describes the scattering of an electromagnetic plane wave by a homogeneous sphere. The solution takes the form of an infinite series of spherical multipole partial waves. It is named after Gustav Mie. The term ''Mie solution'' is also used for solutions of Maxwell's equations for scattering by stratified spheres or by infinite cylinders, or other geometries where one can write separate equations for the radial and angular dependence of solutions. The term ''Mie theory'' is sometimes used for this collection of solutions and methods; it does not refer to an independent physical theory or law. More broadly, the "Mie scattering" formulas are most useful in situations where the size of the scattering particles is comparable to the wavelength of the light, rather than much smaller or much larger. Mie scattering (sometimes referred to as a non-mol ...
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Refractive Index
In optics, the refractive index (or refraction index) of an optical medium is a dimensionless number that gives the indication of the light bending ability of that medium. The refractive index determines how much the path of light is bent, or refracted, when entering a material. This is described by Snell's law of refraction, , where ''θ''1 and ''θ''2 are the angle of incidence and angle of refraction, respectively, of a ray crossing the interface between two media with refractive indices ''n''1 and ''n''2. The refractive indices also determine the amount of light that is reflected when reaching the interface, as well as the critical angle for total internal reflection, their intensity ( Fresnel's equations) and Brewster's angle. The refractive index can be seen as the factor by which the speed and the wavelength of the radiation are reduced with respect to their vacuum values: the speed of light in a medium is , and similarly the wavelength in that medium is , where ''Π...
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Rayleigh–Gans Approximation
Rayleigh–Gans approximation, also known as Rayleigh–Gans–Debye approximation and Rayleigh–Gans–Born approximation, is an approximate solution to light scattering by particles, light scattering by optically soft particles. Optical softness implies that the relative refractive index of particle is close to that of the surrounding medium. The approximation holds for particles of arbitrary shape that are relatively small but can be larger than Rayleigh scattering limits. The theory was derived by John William Strutt, 3rd Baron Rayleigh, Lord Rayleigh in 1881 and was applied to homogeneous spheres, spherical shells, radially inhomogeneous spheres and infinite cylinders. Peter Debye has contributed to the theory in 1881. The theory for homogeneous sphere was rederived by Richard Gans in 1925. The approximation is analogous to Born approximation in quantum mechanics. Theory The validity conditions for the approximation can be denoted as: :, n-1, \ll 1 :kd, n-1, \ll 1 k is the ...
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Rayleigh Scattering
Rayleigh scattering ( ), named after the 19th-century British physicist Lord Rayleigh (John William Strutt), is the predominantly elastic scattering of light or other electromagnetic radiation by particles much smaller than the wavelength of the radiation. For light frequencies well below the resonance frequency of the scattering particle (normal dispersion regime), the amount of scattering is inversely proportional to the fourth power of the wavelength. Rayleigh scattering results from the electric polarizability of the particles. The oscillating electric field of a light wave acts on the charges within a particle, causing them to move at the same frequency. The particle, therefore, becomes a small radiating dipole whose radiation we see as scattered light. The particles may be individual atoms or molecules; it can occur when light travels through transparent solids and liquids, but is most prominently seen in gases. Rayleigh scattering of sunlight in Earth's atmosphere causes d ...
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Reversed-phase Chromatography
Reversed-phase chromatography (also called RPC, reverse-phase chromatography, or hydrophobic chromatography) includes any chromatographic method that uses a hydrophobic stationary phase. RPC refers to liquid (rather than gas) chromatography. Stationary phases In the 1970s, most liquid chromatography was performed using a solid support stationary phase (also called a column) containing unmodified silica or alumina resins. This type of technique is now referred to as normal-phase chromatography. Since the stationary phase is hydrophilic in this technique, molecules with hydrophilic properties contained within the mobile phase will have a high affinity for the stationary phase, and therefore will adsorb to the column packing. Hydrophobic molecules experience less of an affinity for the column packing, and will pass through to be eluted and detected first. Elution of the hydrophilic molecules adsorbed to the column packing requires the use of more hydrophilic or more polar solve ...
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Size Exclusion Chromatography
Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. The chromatography column is packed with fine, porous beads which are commonly composed of dextran, agarose, or polyacrylamide polymers. The pore sizes of these beads are used to estimate the dimensions of macromolecules. SEC is a widely used polymer characterization method because of its ability to provide good molar mass distribution (Mw) results for polymers. Applications The main application of gel-filtration chr ...
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Brookhaven Instruments
Brookhaven may refer to: Places Canada * Amesbury, Toronto, also known as Brookhaven-Amesbury, a Toronto neighbourhood United States * Brookhaven, Georgia, a city just northeast of Atlanta ** Brookhaven/Oglethorpe (MARTA station), a passenger rail station in Brookhaven, Georgia * Brookhaven, Mississippi * Brookhaven, New York, a town in Suffolk County ** Brookhaven (CDP), New York, a hamlet within the town * Brookhaven, Pennsylvania * Brookhaven, Fresno, California, neighborhood Other uses * Brookhaven College, a college in Dallas, Texas * Brookhaven High School (Columbus, Ohio), a high school in Columbus, Ohio * Brookhaven National Laboratory Brookhaven National Laboratory (BNL) is a United States Department of Energy national laboratory located in Upton, Long Island, and was formally established in 1947 at the site of Camp Upton, a former U.S. Army base and Japanese internment c ..., a research facility in Upton, New York * ''Brookhaven RP'', a game on the Roblox platf ...
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Charge-coupled Device
A charge-coupled device (CCD) is an integrated circuit containing an array of linked, or coupled, capacitors. Under the control of an external circuit, each capacitor can transfer its electric charge to a neighboring capacitor. CCD sensors are a major technology used in digital imaging. In a CCD image sensor, pixels are represented by p-doped metal–oxide–semiconductor (MOS) capacitors. These MOS capacitors, the basic building blocks of a CCD, are biased above the threshold for inversion when image acquisition begins, allowing the conversion of incoming photons into electron charges at the semiconductor-oxide interface; the CCD is then used to read out these charges. Although CCDs are not the only technology to allow for light detection, CCD image sensors are widely used in professional, medical, and scientific applications where high-quality image data are required. In applications with less exacting quality demands, such as consumer and professional digital cameras, act ...
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