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Mucor Circinelloides
''Mucor circinelloides'' is a dimorphic fungus belonging to the Order Mucorales (Phylum Mucoromycota). It has a worldwide distribution, found mostly in soil, dung and root vegetables. This species is described as not known to be able to produce mycotoxins, however it has been frequently reported to infect animals such as cattle and swine, as well as fowl, platypus and occasionally humans. Ketoacidotic patients are particularly at risk for infection by ''M. circinelloides''. History and taxonomy ''Mucor circinelloides'' is one of the common species in the genus ''Mucor''. ''Mucor circinelloides'' is a variable species that include several variants such as; ''M. circinelloides'' f. ''circinelloides''; ''M. circinelloides'' f. ''lusitanicus''; ''M. circinelloides'' f. ''griseocyanus'' and ''M. circinelloides'' f. ''janssenii''. Growth and morphology ''Mucor circinelloides'' reproduce asexually. The sporangiophores are found as two types: elongate and sympodially branched. The elon ...
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Philippe Édouard Léon Van Tieghem
Philippe Édouard Léon Van Tieghem (; 19 April 1839 – 28 April 1914) was a French botanist born in Baillleul in the département of Nord. He was one of the best known French botanists of the latter nineteenth century. Life Van Tieghem's father was a textile merchant who died of yellow fever in Martinique before he was born, and his mother shortly thereafter. One of five children, he obtained his ''baccalauréat'' in 1856, and continued his studies at the École Normale Supérieure, where after receiving agrégation, he worked in the laboratory of Louis Pasteur (1822–1895). Here he performed research involving the cultivation of mushrooms. He is credited with creation of the eponymous "Van Tieghem cell", a device mounted on a microscope slide that allows for observing the development of a fungus' mycelium. In 1864 he earned his doctorate in physical sciences with a thesis titled ''Recherches sur la fermentation de l'urée et de l'acide hippurique'', and two years later ob ...
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Subglobose
This glossary of botanical terms is a list of definitions of terms and concepts relevant to botany and plants in general. Terms of plant morphology are included here as well as at the more specific Glossary of plant morphology and Glossary of leaf morphology. For other related terms, see Glossary of phytopathology, Glossary of lichen terms, and List of Latin and Greek words commonly used in systematic names. A B ...
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Mucoraceae
The Mucoraceae are a family of fungi of the order Mucorales, characterized by having the thallus not segmented or ramified. Pathogenic genera include ''Absidia'', ''Apophysomyces'', ''Mucor'', ''Rhizomucor'', and ''Rhizopus''. According to a 2008 estimate, the family contains 25 genera and 129 species. Genera The family consists of the following genera: * '' Actinomucor'' * ''Apophysomyces'' * '' Benjaminiella'' * '' Chaetocladium'' * ''Circinella'' * ''Cokeromyces'' * ''Dicranophora'' * '' Ellisomyces'' * ''Helicostylum'' * '' Hyphomucor'' * '' Kirkomyces'' * ''Mucor'' * '' Parasitella'' * ''Pilaira'' * '' Pilophora'' * '' Pirella'' * ''Rhizomucor'' * '' Rhizopodopsis'' * ''Rhizopus'' * '' Sporodiniella'' * '' Syzygites'' * ''Thamnidium ''Thamnidium'' is a genus of fungi belonging to the family Mucoraceae. The genus was circumscribed in 1809 by Johann Heinrich Friedrich Link. ''Thamnidium'' molds are key participants in the aging process for dry aged beef, producing prote ...
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Nutraceutical
A nutraceutical or bioceutical is a pharmaceutical alternative which claims physiological benefits. In the US, "nutraceuticals" are largely unregulated, as they exist in the same category as dietary supplements and food additives by the FDA, under the authority of the Federal Food, Drug, and Cosmetic Act. Regulation Nutraceuticals are treated differently in different jurisdictions. Canada Under Canadian law, a nutraceutical can either be marketed as a food or as a drug; the terms "nutraceutical" and "functional food" have no legal distinction, referring to "a product isolated or purified from foods that is generally sold in medicinal forms not usually associated with food ndis demonstrated to have a physiological benefit or provide protection against chronic disease." United States The terms "nutraceutical" and 'bioceutical' are not defined by US law. Depending on its ingredients and the claims with which it is marketed, a product is regulated as a drug, dietary supplement, f ...
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Cutaneous
Skin is the layer of usually soft, flexible outer tissue covering the body of a vertebrate animal, with three main functions: protection, regulation, and sensation. Other animal coverings, such as the arthropod exoskeleton, have different developmental origin, structure and chemical composition. The adjective cutaneous means "of the skin" (from Latin ''cutis'' 'skin'). In mammals, the skin is an organ of the integumentary system made up of multiple layers of ectodermal tissue and guards the underlying muscles, bones, ligaments, and internal organs. Skin of a different nature exists in amphibians, reptiles, and birds. Skin (including cutaneous and subcutaneous tissues) plays crucial roles in formation, structure, and function of extraskeletal apparatus such as horns of bovids (e.g., cattle) and rhinos, cervids' antlers, giraffids' ossicones, armadillos' osteoderm, and os penis/os clitoris. All mammals have some hair on their skin, even marine mammals like whales, dolphins, a ...
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MTT Assay
The MTT assay is a colorimetric assay for assessing cell metabolic activity. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present. These enzymes are capable of reducing the tetrazolium dye MTT, which is chemically 3-(4,5- di methyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide, to its insoluble formazan, which has a purple color. Other closely related tetrazolium dyes including XTT, MTS and the WSTs, are used in conjunction with the intermediate electron acceptor, 1-methoxy phenazine methosulfate (PMS). With WST-1, which is cell-impermeable, reduction occurs outside the cell via plasma membrane electron transport. However, this traditionally assumed explanation is currently contended as proof has also been found of MTT reduction to formazan in lipidic cellular structures without apparent involvement of oxidoreductases. Tetrazolium dye assays can also be used to measure cytotoxicity (loss of viable cells) or cy ...
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Cytotoxicity
Cytotoxicity is the quality of being toxic to cells. Examples of toxic agents are an immune cell or some types of venom, e.g. from the puff adder (''Bitis arietans'') or brown recluse spider (''Loxosceles reclusa''). Cell physiology Treating cells with the cytotoxic compound can result in a variety of cell fates. The cells may undergo necrosis, in which they lose membrane integrity and die rapidly as a result of cell lysis. The cells can stop actively growing and dividing (a decrease in cell viability), or the cells can activate a genetic program of controlled cell death (apoptosis). Cells undergoing necrosis typically exhibit rapid swelling, lose membrane integrity, shut down metabolism, and release their contents into the environment. Cells that undergo rapid necrosis in vitro do not have sufficient time or energy to activate apoptotic machinery and will not express apoptotic markers. Apoptosis is characterized by well defined cytological and molecular events including a change i ...
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Water Activity
Water activity (''aw'') is the partial vapor pressure of water in a solution divided by the standard state partial vapor pressure of water. In the field of food science, the standard state is most often defined as pure water at the same temperature. Using this particular definition, pure distilled water has a water activity of exactly one. Water activity is the thermodynamic activity of water as solvent and the relative humidity of the surrounding air after equilibration. As temperature increases, ''aw'' typically increases, except in some products with crystalline salt or sugar. Water migrates from areas of high ''aw'' to areas of low ''aw''. For example, if honey (''aw'' ≈ 0.6) is exposed to humid air (''aw'' ≈ 0.7), the honey hygroscopy, absorbs water from the air. If salami (''aw'' ≈ 0.87) is exposed to dry air (''aw'' ≈ 0.5), the salami desiccation, dries out, which could drying (food), preserve it or staling, spoil it. Lower ''aw'' substances tend to support fewer ...
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Sporulation
In biology, a spore is a unit of sexual or asexual reproduction that may be adapted for dispersal and for survival, often for extended periods of time, in unfavourable conditions. Spores form part of the life cycles of many plants, algae, fungi and protozoa. Bacterial spores are not part of a sexual cycle, but are resistant structures used for survival under unfavourable conditions. Myxozoan spores release amoeboid infectious germs ("amoebulae") into their hosts for parasitic infection, but also reproduce within the hosts through the pairing of two nuclei within the plasmodium, which develops from the amoebula. In plants, spores are usually haploid and unicellular and are produced by meiosis in the sporangium of a diploid sporophyte. Under favourable conditions the spore can develop into a new organism using mitotic division, producing a multicellular gametophyte, which eventually goes on to produce gametes. Two gametes fuse to form a zygote which develops into a new sporo ...
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Petri Dish
A Petri dish (alternatively known as a Petri plate or cell-culture dish) is a shallow transparent lidded dish that biologists use to hold growth medium in which cells can be cultured,R. C. Dubey (2014): ''A Textbook Of Biotechnology For Class-XI'', 4th edition, p. 469. originally, cells of bacteria, fungi and small mosses. The container is named after its inventor, German bacteriologist Julius Richard Petri. It is the most common type of culture plate. The Petri dish is one of the most common items in biology laboratories and has entered popular culture. The term is sometimes written in lower case, especially in non-technical literature. What was later called Petri dish was originally developed by German physician Robert Koch in his private laboratory in 1881, as a precursor method. Petri, as assistant to Koch, at Berlin University made the final modifications in 1887 as used today. Penicillin, the first antibiotic, was discovered in 1929 when Alexander Fleming noticed that m ...
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Czapek Medium
Czapek medium, also called Czapek's agar (CZA) or Czapek-Dox medium, is a growth medium for propagating fungi and other organisms in a laboratory. It was named after its inventors, Czech botanist Friedrich Johann Franz Czapek (May 16, 1868 – July 31, 1921) and American chemist Arthur Wayland Dox (September 19, 1882 – 1954). It was developed to grow ''Aspergillus niger'' and '' Penicillium camemberti''. It works well for many saprophytic fungi and soil bacteria such as species of ''Aspergillus'', '' Candida'', ''Penicillium'', and ''Paecilomyces''. Friedrich Czapek's original recipe is as follows: *1000 g distilled water *30 g cane sugar – energy source and sole source of carbon *1 g dipotassium phosphate – buffering agent *0.5 g magnesium sulfate – source of cations *0.5 g potassium chloride – source of essential ions *0.01 g iron sulfate – source of cations Arthur Wayland Dox added 2 g of sodium nitrate in his version, to p ...
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