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Microbial Mining
Biomining is the technique of extracting metals from ores and other solid materials typically using prokaryotes, fungi or plants ( phytoextraction also known as phytomining or biomining). These organisms secrete different organic compounds that chelate metals from the environment and bring it back to the cell where they are typically used to coordinate electrons. It was discovered in the mid 1900s that microorganisms use metals in the cell. Some microbes can use stable metals such as iron, copper, zinc, and gold as well as unstable atoms such as uranium and thorium. Large chemostats of microbes can be grown to leach metals from their media. These vats of culture can then be transformed into many marketable metal compounds. Biomining is an environmentally friendly technique compared to typical mining. Mining releases many pollutants while the only chemicals released from biomining is any metabolites or gasses that the bacteria secrete. The same concept can be used for bioremediat ...
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Electron Shell 092 Uranium
The electron ( or ) is a subatomic particle with a negative one elementary electric charge. Electrons belong to the first generation of the lepton particle family, and are generally thought to be elementary particles because they have no known components or substructure. The electron's mass is approximately 1/1836 that of the proton. Quantum mechanical properties of the electron include an intrinsic angular momentum (spin) of a half-integer value, expressed in units of the reduced Planck constant, . Being fermions, no two electrons can occupy the same quantum state, in accordance with the Pauli exclusion principle. Like all elementary particles, electrons exhibit properties of both particles and waves: They can collide with other particles and can be diffracted like light. The wave properties of electrons are easier to observe with experiments than those of other particles like neutrons and protons because electrons have a lower mass and hence a longer de Broglie wavelength ...
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South Africa
South Africa, officially the Republic of South Africa (RSA), is the southernmost country in Africa. It is bounded to the south by of coastline that stretch along the South Atlantic and Indian Oceans; to the north by the neighbouring countries of Namibia, Botswana, and Zimbabwe; and to the east and northeast by Mozambique and Eswatini. It also completely enclaves the country Lesotho. It is the southernmost country on the mainland of the Old World, and the second-most populous country located entirely south of the equator, after Tanzania. South Africa is a biodiversity hotspot, with unique biomes, plant and animal life. With over 60 million people, the country is the world's 24th-most populous nation and covers an area of . South Africa has three capital cities, with the executive, judicial and legislative branches of government based in Pretoria, Bloemfontein, and Cape Town respectively. The largest city is Johannesburg. About 80% of the population are Black South Afri ...
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Dissolution (chemistry)
Solvation (or dissolution) describes the interaction of a solvent with dissolved molecules. Both ionized and uncharged molecules interact strongly with a solvent, and the strength and nature of this interaction influence many properties of the solute, including solubility, reactivity, and color, as well as influencing the properties of the solvent such as its viscosity and density. If the attractive forces between the solvent and solute particles are greater than the attractive forces holding the solute particles together, the solvent particles pull the solute particles apart and surround them. The surrounded solute particles then move away from the solid solute and out into the solution. Ions are surrounded by a concentric shell of solvent. Solvation is the process of reorganizing solvent and solute molecules into solvation complexes and involves bond formation, hydrogen bonding, and van der Waals forces. Solvation of a solute by water is called hydration. Solubility of solid ...
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Thermophilic
A thermophile is an organism—a type of extremophile—that thrives at relatively high temperatures, between . Many thermophiles are archaea, though they can be bacteria or fungi. Thermophilic eubacteria are suggested to have been among the earliest bacteria. Thermophiles are found in various geothermally heated regions of the Earth, such as hot springs like those in Yellowstone National Park (see image) and deep sea hydrothermal vents, as well as decaying plant matter, such as peat bogs and compost. Thermophiles can survive at high temperatures, whereas other bacteria or archaea would be damaged and sometimes killed if exposed to the same temperatures. The enzymes in thermophiles function at high temperatures. Some of these enzymes are used in molecular biology, for example the ''Taq'' polymerase used in PCR. "Thermophile" is derived from the el, θερμότητα (''thermotita''), meaning heat, and el, φίλια (''philia''), love. Classification Thermophiles can be c ...
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In Situ
''In situ'' (; often not italicized in English) is a Latin phrase that translates literally to "on site" or "in position." It can mean "locally", "on site", "on the premises", or "in place" to describe where an event takes place and is used in many different contexts. For example, in fields such as physics, geology, chemistry, or biology, ''in situ'' may describe the way a measurement is taken, that is, in the same place the phenomenon is occurring without isolating it from other systems or altering the original conditions of the test. The opposite of ''in situ'' is ''ex situ''. Aerospace In the aerospace industry, equipment on-board aircraft must be tested ''in situ'', or in place, to confirm everything functions properly as a system. Individually, each piece may work but interference from nearby equipment may create unanticipated problems. Special test equipment is available for this ''in situ'' testing. It can also refer to repairs made to the aircraft structure or flight con ...
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Tailings
In mining, tailings are the materials left over after the process of separating the valuable fraction from the uneconomic fraction (gangue) of an ore. Tailings are different to overburden, which is the waste rock or other material that overlies an ore or mineral body and is displaced during mining without being processed. The extraction of minerals from ore can be done two ways: placer mining, which uses water and gravity to concentrate the valuable minerals, or hard rock mining, which pulverizes the rock containing the ore and then relies on chemical reactions to concentrate the sought-after material. In the latter, the extraction of minerals from ore requires comminution, i.e., grinding the ore into fine particles to facilitate extraction of the target element(s). Because of this comminution, tailings consist of a slurry of fine particles, ranging from the size of a grain of sand to a few micrometres. Mine tailings are usually produced from the mill in slurry form, which i ...
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Acidithiobacillus Ferrooxidans
''Acidithiobacillus'' is a genus of the ''Acidithiobacillia'' in the "Pseudomonadota". The genus includes acidophilic organisms capable of iron and/or sulfur oxidation. Like all ''"Pseudomonadota"'', ''Acidithiobacillus'' spp. are Gram-negative. They are also important generators of acid mine drainage, which is a major environmental problem around the world in mining. Genus ''Acidithiobacillus'' ''Acidithiobacillus'' are acidophilic obligate autotrophs (''Acidithiobacillus caldus'' can also grow mixotrophically) that use elementary sulfur, tetrathionate and ferrous iron as electron donors. They assimilate carbon from carbon dioxide using the transaldolase variant of the Calvin-Benson-Bassham cycle. The genus comprises motile, rod-shaped cells that can be isolated from low pH environments including low pH microenvironments on otherwise neutral mineral grains. Phylogeny The order Acidithiobacillales (i.e. ''Thermithiobacillus'') were formerly members of the ''Gammaproteobacteri ...
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Pyrobaculum Islandicum
''Pyrobaculum'' is a genus of the Thermoproteaceae. Description and significance As its Latin name ''Pyrobaculum'' (the "fire stick") suggests, the archaeon is rod-shaped and isolated from locations with high temperatures. It is Gram-negative and its cells are surrounded by an S-layer of protein subunits. ''P. aerophilum'' is a hyperthermophilic and metabolically versatile organism. Different from other hyperthermophiles, it can live in the presence of oxygen and grows efficiently in microaerobic conditions. ''Pyrobaculum yellowstonensis'' strain WP30 was obtained from an elemental sulfur sediment (Joseph's Coat Hot Spring CHS 80 °C, pH 6.1, 135 μM As) in Yellowstone National Park (YNP), USA and is a chemoorganoheterotroph and requires elemental sulfur and/or arsenate as an electron acceptor. Growth in the presence of elemental sulfur and arsenate resulted in the formation of thioarsenates and polysulfides. The complete genome of this organism was sequenced (1.99 Mb, ...
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Pyrococcus Furiosus
''Pyrococcus furiosus'' is a heterotrophic, strictly anaerobic, extremophilic, model species of archaea. It is classified as a hyperthermophile because it thrives best under extremely high temperatures, and is notable for having an optimum growth temperature of 100 °C (a temperature that would destroy most living organisms). ''P. furiosus'' belongs to the '' Pyrococcus'' genus, most commonly found in extreme environmental conditions of hydrothermal vents. It is one of the few prokaryotic organisms that has enzymes containing tungsten, an element rarely found in biological molecules. ''Pyrococcus furiosus'' has many potential industrial applications, owing to its unique thermostable properties. ''P. furiosus'' is used in the process of DNA amplification by way of polymerase chain reaction (PCR) because of its proofreading activity. Utilizing ''P. furiosus'' in PCR DNA amplification instead of the traditionally used ''Taq'' DNA polymerase allows for a significantly more accura ...
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Archaea
Archaea ( ; singular archaeon ) is a domain of single-celled organisms. These microorganisms lack cell nuclei and are therefore prokaryotes. Archaea were initially classified as bacteria, receiving the name archaebacteria (in the Archaebacteria kingdom), but this term has fallen out of use. Archaeal cells have unique properties separating them from the other two domains, Bacteria and Eukaryota. Archaea are further divided into multiple recognized phyla. Classification is difficult because most have not been isolated in a laboratory and have been detected only by their gene sequences in environmental samples. Archaea and bacteria are generally similar in size and shape, although a few archaea have very different shapes, such as the flat, square cells of ''Haloquadratum walsbyi''. Despite this morphological similarity to bacteria, archaea possess genes and several metabolic pathways that are more closely related to those of eukaryotes, notably for the enzymes involved ...
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Leptospirillum Ferrooxidans
Nitrospirota is a phylum of bacteria. It includes multiple genera, such as ''Nitrospira'', the largest. The first member of this phylum, ''Nitrospira marina'', was discovered in 1985. The second member, ''Nitrospira moscoviensis'', was discovered in 1995. Nitrospirota contains nitrifying taxa which oxidize nitrite to nitrate (nitrite-oxidizing bacteria, NOB) and commamox bacteria '' Nitrospira inopinata'' discovered in 2015 and cultivated in 2017. Phylogeny Taxonomy The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LSPN) and the National Center for Biotechnology Information (NCBI). * Phylum "Nitrospirota" Garrity & Holt 2021 ** Class " Leptospirillia" *** Order " Leptospirillales" **** Family " Leptospirillaceae" Cavalier-Smith 2020 ***** Genus '' Leptospirillum'' Markosyan 1972 ex Hippe 2000 ****** Species "'' L. ferrodiazotrophum''" Tyson et al. 2005 ****** Species '' L. ferriphilum'' Coram & Rawlings 2002 ****** Spec ...
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Mesophilic
A mesophile is an organism that grows best in moderate temperature, neither too hot nor too cold, with an optimum growth range from . The optimum growth temperature for these organisms is 37°C. The term is mainly applied to microorganisms. Organisms that prefer extreme environments are known as extremophiles. Mesophiles have diverse classifications, belonging to two domains: Bacteria, Archaea, and to kingdom Fungi of domain Eucarya. Mesophiles belonging to the domain Bacteria can either be gram-positive or gram-negative. Oxygen requirements for mesophiles can be aerobic or anaerobic. There are three basic shapes of mesophiles: coccus, bacillus, and spiral. Habitat The habitats of mesophiles can include cheese and yogurt. They are often included during fermentation of beer and wine making. Since normal human body temperature is 37 °C, the majority of human pathogens are mesophiles, as are most of the organisms comprising the human microbiome. Mesophiles vs. extremophiles ...
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