Marasmius Funalis
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Marasmius Funalis
''Marasmius funalis'' is a species of Marasmiaceae fungus known only from Japan. The species produces small mushrooms with reddish-brown caps up to in diameter and dark-brown, threadlike stems of up to in length. The species has a number of distinctive microscopic features, including very long cystidia on the stem, visible as bristles. Described in 2002 by Haruki Takahashi, the species grows on dead wood. The closest relative of ''M. funalis'' is '' M. liquidambari'', known from Mexico and Papua New Guinea, and it is also similar in appearance to '' M. hudonii'' and '' Setulipes funaliformis'', the latter of which was named after ''M. funalis''. Taxonomy ''Marasmius funalis'' was first described and named in a 2002 article in '' Mycoscience'' by Haruki Takahashi, based on specimens collected in 2000. The specific name, '' funalis'', is Latin for "rope-like", and is in reference to the shape and character of the stem. Within the genus ''Marasmius'', the s ...
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Kanagawa Prefecture
is a prefecture of Japan located in the Kantō region of Honshu. Kanagawa Prefecture is the second-most populous prefecture of Japan at 9,221,129 (1 April 2022) and third-densest at . Its geographic area of makes it fifth-smallest. Kanagawa Prefecture borders Tokyo to the north, Yamanashi Prefecture to the northwest and Shizuoka Prefecture to the west. Yokohama is the capital and largest city of Kanagawa Prefecture and the second-largest city in Japan, with other major cities including Kawasaki, Sagamihara, and Fujisawa. Kanagawa Prefecture is located on Japan's eastern Pacific coast on Tokyo Bay and Sagami Bay, separated by the Miura Peninsula, across from Chiba Prefecture on the Bōsō Peninsula. Kanagawa Prefecture is part of the Greater Tokyo Area, the most populous metropolitan area in the world, with Yokohama and many of its cities being major commercial hubs and southern suburbs of Tokyo. Kanagawa Prefecture was the political and economic center of Japan du ...
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Hemispherical
A sphere () is a Geometry, geometrical object that is a solid geometry, three-dimensional analogue to a two-dimensional circle. A sphere is the Locus (mathematics), set of points that are all at the same distance from a given point in three-dimensional space.. That given point is the centre (geometry), centre of the sphere, and is the sphere's radius. The earliest known mentions of spheres appear in the work of the Greek mathematics, ancient Greek mathematicians. The sphere is a fundamental object in many fields of mathematics. Spheres and nearly-spherical shapes also appear in nature and industry. Bubble (physics), Bubbles such as soap bubbles take a spherical shape in equilibrium. spherical Earth, The Earth is often approximated as a sphere in geography, and the celestial sphere is an important concept in astronomy. Manufactured items including pressure vessels and most curved mirrors and lenses are based on spheres. Spheres rolling, roll smoothly in any direction, so mos ...
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Lugol's Solution
Lugol's iodine, also known as aqueous iodine and strong iodine solution, is a solution of potassium iodide with iodine in water. It is a medication and disinfectant used for a number of purposes. Taken by mouth it is used to treat thyrotoxicosis until surgery can be carried out, protect the thyroid gland from radioactive iodine, and to treat iodine deficiency. When applied to the cervix it is used to help in screening for cervical cancer. As a disinfectant it may be applied to small wounds such as a needle stick injury. A small amount may also be used for emergency disinfection of drinking water. Side effects may include allergic reactions, headache, vomiting, and conjunctivitis. Long term use may result in trouble sleeping and depression. It should not typically be used during pregnancy or breastfeeding. Lugol's iodine is a liquid made up of two parts potassium iodide for every one part elemental iodine in water. Lugol's iodine was first made in 1829 by the French physicia ...
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Melzer's Reagent
Melzer's reagent (also known as Melzer's iodine reagent, Melzer's solution or informally as Melzer's) is a chemical reagent used by mycologists to assist with the identification of fungi, and by phytopathologists for fungi that are plant pathogens. Composition Melzer's reagent is an aqueous solution of chloral hydrate, potassium iodide, and iodine. Depending on the formulation, it consists of approximately 2.50-3.75% potassium iodide and 0.75–1.25% iodine, with the remainder of the solution being 50% water and 50% chloral hydrate. Melzer's is toxic to humans if ingested due to the presence of iodine and chloral hydrate. Due to the legal status of chloral hydrate, Melzer's reagent is difficult to obtain in the United States. In response to difficulties obtaining chloral hydrate, scientists at Rutgers formulated Visikol (compatible with Lugol's iodine) as a replacement. In 2019, research showed that Visikol behaves differently to Melzer’s reagent in several key situations, notin ...
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Iodine
Iodine is a chemical element with the symbol I and atomic number 53. The heaviest of the stable halogens, it exists as a semi-lustrous, non-metallic solid at standard conditions that melts to form a deep violet liquid at , and boils to a violet gas at . The element was discovered by the French chemist Bernard Courtois in 1811 and was named two years later by Joseph Louis Gay-Lussac, after the Ancient Greek 'violet-coloured'. Iodine occurs in many oxidation states, including iodide (I−), iodate (), and the various periodate anions. It is the least abundant of the stable halogens, being the sixty-first most abundant element. As the heaviest essential mineral nutrient, iodine is required for the synthesis of thyroid hormones. Iodine deficiency affects about two billion people and is the leading preventable cause of intellectual disabilities. The dominant producers of iodine today are Chile and Japan. Due to its high atomic number and ease of attachment to organic compound ...
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Staining
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells. In biochemistry, it involves adding a class-specific ( DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for viewin ...
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Inamyloid
In mycology a tissue or feature is said to be amyloid if it has a positive amyloid reaction when subjected to a crude chemical test using iodine as an ingredient of either Melzer's reagent or Lugol's solution, producing a blue to blue-black staining. The term "amyloid" is derived from the Latin ''amyloideus'' ("starch-like"). It refers to the fact that starch gives a similar reaction, also called an amyloid reaction. The test can be on microscopic features, such as spore walls or hyphal walls, or the apical apparatus or entire ascus wall of an ascus, or be a macroscopic reaction on tissue where a drop of the reagent is applied. Negative reactions, called inamyloid or nonamyloid, are for structures that remain pale yellow-brown or clear. A reaction producing a deep reddish to reddish-brown staining is either termed a dextrinoid reaction (pseudoamyloid is a synonym) or a hemiamyloid reaction. Melzer's reagent reactions Hemiamyloidity Hemiamyloidity in mycology refers to a special ...
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Cell Wall
A cell wall is a structural layer surrounding some types of cells, just outside the cell membrane. It can be tough, flexible, and sometimes rigid. It provides the cell with both structural support and protection, and also acts as a filtering mechanism. Cell walls are absent in many eukaryotes, including animals, but are present in some other ones like fungi, algae and plants, and in most prokaryotes (except mollicute bacteria). A major function is to act as pressure vessels, preventing over-expansion of the cell when water enters. The composition of cell walls varies between taxonomic group and species and may depend on cell type and developmental stage. The primary cell wall of land plants is composed of the polysaccharides cellulose, hemicelluloses and pectin. Often, other polymers such as lignin, suberin or cutin are anchored to or embedded in plant cell walls. Algae possess cell walls made of glycoproteins and polysaccharides such as carrageenan and agar that are absent ...
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Micrometre
The micrometre ( international spelling as used by the International Bureau of Weights and Measures; SI symbol: μm) or micrometer (American spelling), also commonly known as a micron, is a unit of length in the International System of Units (SI) equalling (SI standard prefix "micro-" = ); that is, one millionth of a metre (or one thousandth of a millimetre, , or about ). The nearest smaller common SI unit is the nanometre, equivalent to one thousandth of a micrometre, one millionth of a millimetre or one billionth of a metre (). The micrometre is a common unit of measurement for wavelengths of infrared radiation as well as sizes of biological cells and bacteria, and for grading wool by the diameter of the fibres. The width of a single human hair ranges from approximately 20 to . The longest human chromosome, chromosome 1, is approximately in length. Examples Between 1 μm and 10 μm: * 1–10 μm – length of a typical bacterium * 3–8 μm – width of ...
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Ellipsoidal
An ellipsoid is a surface that may be obtained from a sphere by deforming it by means of directional scalings, or more generally, of an affine transformation. An ellipsoid is a quadric surface;  that is, a surface that may be defined as the zero set of a polynomial of degree two in three variables. Among quadric surfaces, an ellipsoid is characterized by either of the two following properties. Every planar cross section is either an ellipse, or is empty, or is reduced to a single point (this explains the name, meaning "ellipse-like"). It is bounded, which means that it may be enclosed in a sufficiently large sphere. An ellipsoid has three pairwise perpendicular axes of symmetry which intersect at a center of symmetry, called the center of the ellipsoid. The line segments that are delimited on the axes of symmetry by the ellipsoid are called the ''principal axes'', or simply axes of the ellipsoid. If the three axes have different lengths, the figure is a triaxial ellipsoid (ra ...
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Basidiospore
A basidiospore is a reproductive spore produced by Basidiomycete fungi, a grouping that includes mushrooms, shelf fungi, rusts, and smuts. Basidiospores typically each contain one haploid nucleus that is the product of meiosis, and they are produced by specialized fungal cells called basidia. Typically, four basidiospores develop on appendages from each basidium, of which two are of one strain and the other two of its opposite strain. In gills under a cap of one common species, there exist millions of basidia. Some gilled mushrooms in the order Agaricales have the ability to release billions of spores. The puffball fungus ''Calvatia gigantea'' has been calculated to produce about five trillion basidiospores. Most basidiospores are forcibly discharged, and are thus considered ballistospores. These spores serve as the main air dispersal units for the fungi. The spores are released during periods of high humidity and generally have a night-time or pre-dawn peak concentration in the ...
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Spore Print
300px, Making a spore print of the mushroom ''Volvariella volvacea'' shown in composite: (photo lower half) mushroom cap laid on white and dark paper; (photo upper half) cap removed after 24 hours showing pinkish-tan spore print. A 3.5-centimeter glass slide placed in middle allows for examination of spore characteristics under a microscope. image:spore Print ID.gif, 300px, A printable chart to make a spore print and start identification The spore print is the powdery deposit obtained by allowing spores of a fungal sporocarp (fungi), fruit body to fall onto a surface underneath. It is an important diagnostic character in most handbooks for identifying mushrooms. It shows the colour of the mushroom spores if viewed en masse. Method A spore print is made by placing the spore-producing surface flat on a sheet of dark and white paper or on a sheet of clear, stiff plastic, which facilitates moving the spore print to a darker or lighter surface for improved contrast; for example, it ...
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