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MacConkey Agar
MacConkey agar is a selective and differential culture medium for bacteria. It is designed to selectively isolate gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation. Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color. The media inhibits growth of gram-positive organisms with crystal violet and bile salts, allowing for the selection and isolation of gram-negative bacteria. The media detects lactose fermentation by enteric bacteria with the pH indicator neutral red. Contents It contains bile salts (to inhibit most gram-positive bacteria), crystal violet dye (which also inhibits certain gram-positive bacteria), and neutral red dye (which turns pink if the microbes are fermenting lactose). Composition: * Peptone – 17 g * Proteose peptone – 3 g * Lactose – 10 g * Bile salts – 1.5 g * Sodium chloride – 5 g * Neutral red – 0.03 g * Crystal violet – ...
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Proteus McConkey
In Greek mythology, Proteus ( ; ) is an early prophetic sea god or god of rivers and oceanic bodies of water, one of several deities whom Homer calls the "Old Man of the Sea" (''hálios gérôn''). Some who ascribe a specific domain to Proteus call him the god of "elusive sea change", which suggests the changeable nature of the sea or the liquid quality of water. He can foretell the future, but, in a mytheme familiar to several cultures, will change his shape to avoid doing so; he answers only to those who are capable of capturing him. From this feature of Proteus comes the adjective protean, meaning "versatile", "mutable", or "capable of assuming many forms". "Protean" has positive connotations of flexibility, versatility and adaptability. Name origin Proteus's name suggests the "first" (from Greek language, Greek "" , "first"), as () is the "primordial" or the "firstborn". It is not certain to what this refers, but in myths where he is the son of Poseidon, it possibly refe ...
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Bile Salts
Bile acids are steroid acids found predominantly in the bile of mammals and other vertebrates. Diverse bile acids are synthesized in the liver in peroxisomes. Bile acids are conjugated with taurine or glycine residues to give anions called bile salts. Primary bile acids are those synthesized by the liver. Secondary bile acids result from bacterial actions in the colon (anatomy), colon. In humans, taurocholic acid and glycocholic acid (derivatives of cholic acid) and taurochenodeoxycholic acid and glycochenodeoxycholic acid (derivatives of chenodeoxycholic acid) are the major bile salts. The salts of their 7-alpha-dehydroxylated derivatives, deoxycholic acid and lithocholic acid, are also found, with derivatives of cholic, chenodeoxycholic and deoxycholic acids accounting for over 90% of human biliary bile acids. Description Bile acids comprise about 80% of the organic compounds in bile (others are phospholipids and cholesterol). An increased secretion of bile acids produces an ...
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Klebsiella
''Klebsiella'' is a genus of Gram-negative, oxidase-negative, rod-shaped bacteria with a prominent polysaccharide-based capsule. ''Klebsiella'' is named after German-Swiss microbiologist Edwin Klebs (1834–1913). Carl Friedlander described ''Klebsiella'' bacillus which is why it was termed ''Friedlander bacillus'' for many years. The species of ''Klebsiella'' are all gram-negative and usually non-motile. They tend to be shorter and thicker when compared to others in the family Enterobacteriaceae. ''Klebsiella'' species are found everywhere in nature. This is thought to be due to distinct sublineages developing specific niche adaptations, with associated biochemical adaptations which make them better suited to a particular environment. They can be found in water, soil, plants, insects and other animals including humans, including as part of the human and animal's normal flora in the nose, mouth and intestines. List of species * ''K. aerogenes'', previously known as ''Enter ...
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Enterobacter
''Enterobacter'' is a genus of common Gram-negative, Facultative anaerobic organism, facultatively anaerobic, bacillus (shape), rod-shaped, non-spore-forming bacteria of the family Enterobacteriaceae. Cultures are found in soil, water, sewage, feces and gut environments. It is the type genus of the order Enterobacterales. Several strains of these bacteria are pathogenic and cause opportunistic infections in immunocompromised (usually hospitalized) hosts and in those who are on mechanical ventilation. The urinary tract, urinary and respiratory tracts are the most common sites of infection. The genus ''Enterobacter'' is a member of the coliform group of bacteria. It does not belong to the fecal coliforms (or thermotolerant coliforms) group of bacteria, unlike ''Escherichia coli'', because it is incapable of growth at 44.5 °C in the presence of bile salts. Some of them show quorum sensing properties. One clinically important species from this genus is ''Enterobacter cloac ...
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Escherichia Coli
''Escherichia coli'' ( )Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. is a gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus '' Escherichia'' that is commonly found in the lower intestine of warm-blooded organisms. Most ''E. coli'' strains are part of the normal microbiota of the gut, where they constitute about 0.1%, along with other facultative anaerobes. These bacteria are mostly harmless or even beneficial to humans. For example, some strains of ''E. coli'' benefit their hosts by producing vitamin K2 or by preventing the colonization of the intestine by harmful pathogenic bacteria. These mutually beneficial relationships between ''E. coli'' and humans are a type of mutualistic biological relationship—where both the humans and the ''E. coli'' are benefitting each other. ''E. coli'' is expelled into the environment within fecal matter. The bacterium grows massi ...
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American Society For Microbiology
The American Society for Microbiology (ASM), originally the Society of American Bacteriologists, is a professional organization for scientists who study viruses, bacteria, fungi, algae, and protozoa as well as other aspects of microbiology. It was founded in 1899. The Society publishes a variety of scientific journals, textbooks, and other educational materials related to microbiology and infectious diseases. ASM organizes annual meetings, as well as workshops and professional development opportunities for its members. History ASM was founded in 1899 under the name the "Society of American Bacteriologists." In December 1960, it was renamed the "American Society for Microbiology." Mission ASM's mission is "to promote and advance the microbial sciences." The society seeks to accomplish this mission through: * Publishing highly cited publications * Running multi-disciplinary meetings * Deploying resources and expertise around the world * Advocating for scientific research * Fosteri ...
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Royal Commission On Sewage Disposal
{{Use dmy dates, date=April 2022 The Royal Commission on Sewage Disposal was established by the British government in 1898 to report on: (1) What method or methods of treating and disposing of sewage (including any liquid from any factory, or manufacturing process) may properly be adopted, consistently with due regard for the requirements of the existing law, for the protection of the public health, and for the economical and efficient discharge of the duties of local, authorities; and (2) If more than one method may be so adopted, by what rules, in relation to the nature or volume of sewage, or the population to be served, or other varying circumstances or requirements, should the particular method of treatment and disposal to be adopted be determined The commission convened and re-convened eight times under three different reigns, Victoria, Edward VII and King George V, and remained active until 1912 and in that period published nine reports. *The first report (Cd. 685), publis ...
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Bacteriologist
A bacteriologist is a microbiologist, or similarly trained professional, in bacteriology— a subdivision of microbiology that studies bacteria, typically Pathogenic bacteria, pathogenic ones. Bacteriologists are interested in studying and learning about bacteria, as well as using their skills in clinical settings. This includes investigating properties of bacteria such as Morphology (biology), morphology, ecology, genetics and biochemistry, phylogenetics, genomics and many other areas related to bacteria like Medical diagnosis, disease diagnostic testing. Alongside human and animal health care, healthcare providers, they may carry out various functions as Biomedical scientist, medical scientists, veterinary scientists, Pathology, pathologists, or Medical laboratory scientist, diagnostic technicians in locations like Clinic, clinics, blood banks, hospitals, laboratories and Veterinary medicine, animal hospitals. Bacteriologists working in public health or biomedical research help de ...
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Alfred Theodore MacConkey
Alfred Theodore MacConkey (1861-17 May 1931) was a British bacteriologist. He developed MacConkey's agar, a selective medium that is used in the diagnosis of enteric pathogens. He was born McConkey but appears to have spelled his name "MacConkey" from at least 1881 and in all his published papers Early life and education MacConkey was the youngest of six children born to West Derby minister Andrew McConkey and wife Margaret. He matriculated at Liverpool Collegiate Institution in 1880, studied Natural Sciences at Gonville and Caius College, Cambridge and Medicine at Guy's Hospital, completing his studies in 1889. Medical practice and early scientific career He initially went into private practice at Beckenham, Kent, but after illness decided to specialize in bacteriology, joining the Bacteriology department at Guy's Hospital in 1897. This is where he started to develop the culture medium that bears his name. His contemporaries included Herbert Durham. Liverpool and the Royal Co ...
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Sodium Taurocholate
Taurocholic acid, known also as cholaic acid, cholyltaurine, or acidum cholatauricum, is a deliquescent yellowish crystalline bile acid involved in the emulsification of fats. It occurs as a sodium salt in the bile of mammals. It is a conjugate of cholic acid with taurine. In medical use, it is administered as a cholagogue and choleretic. Hydrolysis of taurocholic acid yields taurine. For commercial use, taurocholic acid is manufactured from cattle bile, a byproduct of the meat-processing industry. This acid is also one of the many molecules in the body that has cholesterol as its precursor. In a large prospective study (involving 569 incident colon cancer cases and 569 matched controls) it was found that prediagnostic concentrations of circulating taurocholic acid, as well as six other bile acids, were statistically significantly associated with increased colon cancer risk. Toxicity The median lethal dose of taurocholic acid in newborn rats is 380 mg/kg. See also * Deoxycho ...
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Water
Water is an inorganic compound with the chemical formula . It is a transparent, tasteless, odorless, and Color of water, nearly colorless chemical substance. It is the main constituent of Earth's hydrosphere and the fluids of all known living organisms (in which it acts as a solvent). It is vital for all known forms of life, despite not providing food energy or organic micronutrients. Its chemical formula, , indicates that each of its molecules contains one oxygen and two hydrogen atoms, connected by covalent bonds. The hydrogen atoms are attached to the oxygen atom at an angle of 104.45°. In liquid form, is also called "water" at standard temperature and pressure. Because Earth's environment is relatively close to water's triple point, water exists on Earth as a solid, a liquid, and a gas. It forms precipitation in the form of rain and aerosols in the form of fog. Clouds consist of suspended droplets of water and ice, its solid state. When finely divided, crystalline ice ...
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Agar
Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from " ogonori" and " tengusa". As found in nature, agar is a mixture of two components, the linear polysaccharide agarose and a heterogeneous mixture of smaller molecules called agaropectin. It forms the supporting structure in the cell walls of certain species of algae and is released on boiling. These algae are known as agarophytes, belonging to the Rhodophyta (red algae) phylum. The processing of food-grade agar removes the agaropectin, and the commercial product is essentially pure agarose. Agar has been used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture media for microbiological work. Agar can be used as a laxative; an appetite suppressant; a vegan substitute for gelatin; a thickener for soups; in fruit preserves, ice cream, and other desserts; as a clarifying ...
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