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Live Cell Imaging
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. Live-cell imaging was pioneered in the first decade of the 21st century. One of the first time-lapse microcinematographic films of cells ever made was made by Julius Ries, showing the fertilization and development of the sea urchin egg. Since then, several microscopy methods have been developed to study living cells in greater detail with less effort. A newer type of imaging using quantum dots have been used, as they are shown to be more stable. The development of holotomographic microscopy has disregarded phototoxicity and other staining-derived disadvantages by implementing digital staining based on cells’ refractive index. Overview Biological systems exist as a complex interplay of countless cellular components interacting across four dimensions to produce the phenomenon called ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Olympus FluoView FV1000 Confocal Microscope - NCMIR
Olympus or Olympos ( grc, Ὄλυμπος, link=no) may refer to: Mountains In antiquity Greece * Mount Olympus in Thessaly, northern Greece, the home of the twelve gods of Olympus in Greek mythology * Mount Olympus (Lesvos), located in Lesbos * Mount Olympus (Euboea), located in Euboea * Mount Olympus (Attica), located in East Attica * Mount Olympus (Skyros), located in Skyros * Mount Lykaion, located in Arcadia Turkey * Mysian Olympus (present-day Uludağ), in northwest Turkey * Paphlagonian Olympus (present-day Arıt Dağı near Bartın) * Mount Nif (present-day Nif Dağı in Aegean Turkey) * Lycian Olympus (present-day Tahtalı Dağı near Kemer) Cyprus * Mount Olympus (Cyprus), the highest point (1952 m) on the island of Cyprus In modern times United States * Mount Olympus (Washington), on the Olympic Peninsula * Mount Olympus (Utah), on the Wasatch Front * Mount Olympus (San Francisco), in the Ashbury Heights neighborhood New Zealand * Mount Olympus ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Light Microscope
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope. In high-power microscopes, both eyepieces typically show the same image, but with a stereo microscope, slightly different images are used to create a 3-D effect. A camera is typically used to capture the image (micrograph). The sample can be lit in a variety of ways. Transparent objects can be lit from below and solid objects can be lit with light coming through ( bright field) or around (dark field) the objective lens. Polarised ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Inverse Scattering
In mathematics and physics, the inverse scattering problem is the problem of determining characteristics of an object, based on data of how it scatters incoming radiation or particles. It is the inverse problem to the direct scattering problem, which is to determine how radiation or particles are scattered based on the properties of the scatterer. Soliton equations are a class of partial differential equations which can be studied and solved by a method called the inverse scattering transform, which reduces the nonlinear PDEs to a linear inverse scattering problem. The nonlinear Schrödinger equation, the Korteweg–de Vries equation and the KP equation are examples of soliton equations. In one space dimension the inverse scattering problem is equivalent to a Riemann-Hilbert problem. Since its early statement for radiolocation, many applications have been found for inverse scattering techniques, including echolocation, geophysical Geophysics () is a subject of natural sci ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Holography
Holography is a technique that enables a wavefront to be recorded and later re-constructed. Holography is best known as a method of generating real three-dimensional images, but it also has a wide range of other applications. In principle, it is possible to make a hologram for any type of wave. A hologram is made by superimposing a second wavefront (normally called the reference beam) on the wavefront of interest, thereby generating an interference pattern which is recorded on a physical medium. When only the second wavefront illuminates the interference pattern, it is diffracted to recreate the original wavefront. Holograms can also be computer-generated by modelling the two wavefronts and adding them together digitally. The resulting digital image is then printed onto a suitable mask or film and illuminated by a suitable source to reconstruct the wavefront of interest. Overview and history The Hungarian- British physicist Dennis Gabor (in Hungarian: ''Gábor Déne ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Refractive Index
In optics, the refractive index (or refraction index) of an optical medium is a dimensionless number that gives the indication of the light bending ability of that medium. The refractive index determines how much the path of light is bent, or refracted, when entering a material. This is described by Snell's law of refraction, , where ''θ''1 and ''θ''2 are the angle of incidence and angle of refraction, respectively, of a ray crossing the interface between two media with refractive indices ''n''1 and ''n''2. The refractive indices also determine the amount of light that is reflected when reaching the interface, as well as the critical angle for total internal reflection, their intensity ( Fresnel's equations) and Brewster's angle. The refractive index can be seen as the factor by which the speed and the wavelength of the radiation are reduced with respect to their vacuum values: the speed of light in a medium is , and similarly the wavelength in that medium is , where ''Π... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Holotomography
Holotomography (HT) is a laser technique to measure three-dimensional refractive index (RI) tomogram of a microscopic sample such as biological cells and tissues. Because the RI can serve as an intrinsic imaging contrast for transparent or phase objects, measurements of RI tomograms can provide label-free quantitative imaging of microscopic phase objects. In order to measure 3-D RI tomogram of samples, HT employs the principle of holographic imaging and inverse scattering. Typically, multiple 2D holographic images of a sample are measured at various illumination angles, employing the principle of interferometric imaging. Then, a 3D RI tomogram of the sample is reconstructed from these multiple 2D holographic images by inversely solving light scattering in the sample. History The first theoretical proposal was presented by Emil Wolf, and the first experimental demonstration was shown by Fercher et al. From 2000s, HT techniques had been extensively studied and applied to the field ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Image Sensor
An image sensor or imager is a sensor that detects and conveys information used to make an image. It does so by converting the variable attenuation of light waves (as they pass through or reflect off objects) into signals, small bursts of current that convey the information. The waves can be light or other electromagnetic radiation. Image sensors are used in electronic imaging devices of both analog and digital types, which include digital cameras, camera modules, camera phones, optical mouse devices, medical imaging equipment, night vision equipment such as thermal imaging devices, radar, sonar, and others. As technology changes, electronic and digital imaging tends to replace chemical and analog imaging. The two main types of electronic image sensors are the charge-coupled device (CCD) and the active-pixel sensor (CMOS sensor). Both CCD and CMOS sensors are based on metal–oxide–semiconductor (MOS) technology, with CCDs based on MOS capacitors and CMOS sensors based on M ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Digital Holographic Microscopy Video Showing Cell Division Of Unlabeled JIMT-1 Breast Cancer Cells
Digital usually refers to something using discrete digits, often binary digits. Technology and computing Hardware *Digital electronics, electronic circuits which operate using digital signals **Digital camera, which captures and stores digital images ***Digital versus film photography **Digital computer, a computer that handles information represented by discrete values **Digital recording, information recorded using a digital signal Socioeconomic phenomena *Digital culture, the anthropological dimension of the digital social changes * Digital divide, a form of economic and social inequality in access to or use of information and communication technologies * Digital economy, an economy based on computing and telecommunications resources Other uses in technology and computing * Digital data, discrete data, usually represented using binary numbers *Digital marketing, search engine & social media presence booster, usually represented using online visibility. * Digital media, media ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Phototoxicity
Phototoxicity, also called photoirritation, is a chemically induced skin irritation, requiring light, that does not involve the immune system. It is a type of photosensitivity. The skin response resembles an exaggerated sunburn. The involved chemical may enter into the skin by topical administration or it may reach the skin via systemic circulation following ingestion or parenteral administration. The chemical needs to be "photoactive," which means that when it absorbs light, the absorbed energy produces molecular changes that cause toxicity. Many synthetic compounds, including drug substances like tetracyclines or fluoroquinolones, are known to cause these effects. Surface contact with some such chemicals causes photodermatitis; many plants cause phytophotodermatitis. Light-induced toxicity is a common phenomenon in humans; however, it also occurs in other animals. Scientific background A phototoxic substance is a chemical compound which becomes toxic when exposed to light. * So ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorophore
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions). More generally they are covalently bonded to a macromolecule, serving as a marker (or dye, or tag, or reporter) for affine or bioactive reagents (antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, i.e., fluorescent imaging and spectroscopy. Fluorescein, via its amine-reactive isothiocyanate derivative fluorescein isothiocyanate (FITC), has been one of the most popular fluorophores. Fro ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Time-lapse Video Of 16-cell Purple Urchin Embryo, CIL15792
Time-lapse photography is a technique in which the frequency at which film frames are captured (the frame rate) is much lower than the frequency used to view the sequence. When played at normal speed, time appears to be moving faster and thus ''lapsing''. For example, an image of a scene may be captured at 1 frame per second but then played back at 30 frames per second; the result is an apparent ''30 times'' speed increase. Similarly, film can also be played at a much lower rate than at which it was captured, which slows down an otherwise fast action, as in slow motion or high-speed photography. Processes that would normally appear subtle and slow to the human eye, such as the motion of the sun and stars in the sky or the growth of a plant, become very pronounced. Time-lapse is the extreme version of the cinematography technique of ''undercranking''. Stop motion animation is a comparable technique; a subject that does not actually move, such as a puppet, can repeatedly be ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
