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Laminar Flow Cabinet
A laminar flow cabinet or tissue culture hood is a carefully enclosed bench designed to prevent contamination of semiconductor wafers, biological samples, or any particle sensitive materials. Air is drawn through a HEPA filter and blown in a very smooth, laminar flow towards the user. Due to the direction of air flow, the sample is protected from the user but the user is not protected from the sample. The cabinet is usually made of stainless steel with no gaps or joints where spores might collect. Such hoods exist in both horizontal and vertical configurations, and there are many different types of cabinets with a variety of airflow patterns and acceptable uses. Laminar flow cabinets may have a UV-C germicidal lamp to sterilize the interior and contents before usage to prevent contamination of the experiment. Germicidal lamps are usually kept on for fifteen minutes to sterilize the interior before the cabinet is used. The light must be switched off when the cabinet is being ...
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Particulate
Particulates – also known as atmospheric aerosol particles, atmospheric particulate matter, particulate matter (PM) or suspended particulate matter (SPM) – are microscopic particles of solid or liquid matter suspended in the air. The term ''aerosol'' commonly refers to the particulate/air mixture, as opposed to the particulate matter alone. Sources of particulate matter can be natural or anthropogenic. They have impacts on climate and precipitation that adversely affect human health, in ways additional to direct inhalation. Types of atmospheric particles include suspended particulate matter; thoracic and respirable particles; inhalable coarse particles, designated PM, which are coarse particles with a diameter of 10 micrometers (μm) or less; fine particles, designated PM, with a diameter of 2.5 μm or less; ultrafine particles, with a diameter of 100 nm or less; and soot. The IARC and WHO designate airborne particulates as a Group 1 carcinogen. Particula ...
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Biosafety Cabinet
A biosafety cabinet (BSC)—also called a biological safety cabinet or microbiological safety cabinet—is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with (or potentially contaminated with) pathogens requiring a defined biosafety level. Several different types of BSC exist, differentiated by the degree of biocontainment they provide. BSCs first became commercially available in 1950. Purposes The primary purpose of a BSC is to serve as a means to protect the laboratory worker and the surrounding environment from pathogens. All exhaust air is HEPA-filtered as it exits the biosafety cabinet, removing harmful bacteria and viruses. This is in contrast to a laminar flow clean bench, which blows unfiltered exhaust air towards the user and is not safe for work with pathogenic agents. Neither are most BSCs safe for use as fume hoods. Likewise, a fume hood fails to provide the environmental protection that HEPA filtration in a BSC wou ...
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Fume Hood
A fume hood (sometimes called a fume cupboard or fume closet) is a type of local ventilation device that is designed to limit exposure to hazardous or toxic fumes, vapors or dusts. Description A fume hood is typically a large piece of equipment enclosing five sides of a work area, the bottom of which is most commonly located at a standing work height. Two main types exist, ducted and recirculating (ductless). The principle is the same for both types: air is drawn in from the front (open) side of the cabinet, and either expelled outside the building or made safe through filtration and fed back into the room. This is used to: * protect the user from inhaling toxic gases (fume hoods, biosafety cabinets, glove boxes) * protect the product or experiment (biosafety cabinets, glove boxes) * protect the environment (recirculating fume hoods, certain biosafety cabinets, and any other type when fitted with appropriate filters in the exhaust airstream) Secondary functions of these devic ...
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Preparation Of Microbiological Samples In A Laminar Chamber
Preparation may refer to: * Preparation (dental), the method by which a tooth is prepared when removing decay and designing a form that will provide adequate retention for a dental restoration * Preparation (music), treatment of dissonance in tonal music * Preparation, Iowa, a ghost town * Preparation time, time to prepare speeches in policy debate * ''The Preparation'', a 2017 South Korean film * ''Preparations'' (album), a 2007 album by Prefuse 73 * Prepared dosage form * Prepared drug * Prepared food * Prepared supplement * Special modifications to instruments, see **Prepared piano **Prepared guitar *Fossil preparation See also * Preparation H, popular hemorrhoids medicine * Preparation for the Gospel, early Christian book * * Prep (other) * Preparationism Preparationism is the view in Christian theology that unregenerate people can take steps in preparation for conversion, and should be exhorted to do so. Preparationism advocates a series of things that people ...
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HEPA
HEPA (, high-efficiency particulate air) filter, also known as high-efficiency particulate absorbing filter and high-efficiency particulate arrestance filter, is an efficiency standard of air filters. Filters meeting the HEPA standard must satisfy certain levels of efficiency. Common standards require that a HEPA air filter must remove—from the air that passes through—at least 99.95% (ISO, European Standard) or 99.97% (ASME, U.S. DOE) of particles whose diameter is equal to 0.3  μm, with the filtration efficiency increasing for particle diameters both less than and greater than 0.3 μm. HEPA filters capture pollen, dirt, dust, moisture, bacteria (0.2-2.0 μm), virus (0.02-0.3 μm), and submicron liquid aerosol (0.02-0.5 μm). Some microorganisms, for example, ''Aspergillus niger'', '' Penicillium citrinum'', '' Staphylococcus epidermidis'', and ''Bacillus subtilis'' are captured by HEPA filters with photocatalytic oxidation (PCO). A HEPA filt ...
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Laminar Flow
In fluid dynamics, laminar flow is characterized by fluid particles following smooth paths in layers, with each layer moving smoothly past the adjacent layers with little or no mixing. At low velocities, the fluid tends to flow without lateral mixing, and adjacent layers slide past one another like playing cards. There are no cross-currents perpendicular to the direction of flow, nor eddies or swirls of fluids. In laminar flow, the motion of the particles of the fluid is very orderly with particles close to a solid surface moving in straight lines parallel to that surface. Laminar flow is a flow regime characterized by high momentum diffusion and low momentum convection. When a fluid is flowing through a closed channel such as a pipe or between two flat plates, either of two types of flow may occur depending on the velocity and viscosity of the fluid: laminar flow or turbulent flow. Laminar flow occurs at lower velocities, below a threshold at which the flow becomes turbulent. ...
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Stainless Steel
Stainless steel is an alloy of iron that is resistant to rusting and corrosion. It contains at least 11% chromium and may contain elements such as carbon, other nonmetals and metals to obtain other desired properties. Stainless steel's resistance to corrosion results from the chromium, which forms a passive film that can protect the material and self-heal in the presence of oxygen. The alloy's properties, such as luster and resistance to corrosion, are useful in many applications. Stainless steel can be rolled into sheets, plates, bars, wire, and tubing. These can be used in cookware, cutlery, surgical instruments, major appliances, vehicles, construction material in large buildings, industrial equipment (e.g., in paper mills, chemical plants, water treatment), and storage tanks and tankers for chemicals and food products. The biological cleanability of stainless steel is superior to both aluminium and copper, having a biological cleanability comparable to glass ...
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Airflow
Airflow, or air flow, is the movement of air. The primary cause of airflow is the existence of air. Air behaves in a fluid manner, meaning particles naturally flow from areas of higher pressure to those where the pressure is lower. Atmospheric air pressure is directly related to altitude, temperature, and composition. In engineering, airflow is a measurement of the amount of air per unit of time that flows through a particular device. It can be described as a volumetric flow rate (volume of air per unit time) or a mass flow rate (mass of air per unit time). What relates both forms of description is the air density, which is a function of pressure and temperature through the ideal gas law. The flow of air can be induced through mechanical means (such as by operating an electric or manual fan) or can take place passively, as a function of pressure differentials present in the environment. Types of airflow Like any fluid, air may exhibit both laminar and turbulent flow patterns ...
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UV-C
Ultraviolet (UV) is a form of electromagnetic radiation with wavelength from 10 nm (with a corresponding frequency around 30  PHz) to 400 nm (750  THz), shorter than that of visible light, but longer than X-rays. UV radiation is present in sunlight, and constitutes about 10% of the total electromagnetic radiation output from the Sun. It is also produced by electric arcs and specialized lights, such as mercury-vapor lamps, tanning lamps, and black lights. Although long-wavelength ultraviolet is not considered an ionizing radiation because its photons lack the energy to ionize atoms, it can cause chemical reactions and causes many substances to glow or fluoresce. Consequently, the chemical and biological effects of UV are greater than simple heating effects, and many practical applications of UV radiation derive from its interactions with organic molecules. Short-wave ultraviolet light damages DNA and sterilizes surfaces with which it comes into contact. For hu ...
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Germicidal Lamp
A germicidal lamp (also known as disinfection lamp or sterilizer lamp) is an electric light that produces ultraviolet C (UVC) light. This short-wave ultraviolet light disrupts DNA base pairing, causing formation of pyrimidine dimers, and leads to the inactivation of bacteria, viruses, and protozoans. It can also be used to produce ozone for water disinfection. They are used in ultraviolet germicidal irradiation (UVGI). There are four common types available: * Low-pressure mercury lamps * High-pressure mercury lamps * Excimer lamps * LEDs Low-pressure mercury lamps Low-pressure mercury lamps are very similar to a fluorescent lamp, with a wavelength of 253.7 nm (1182.5 THz). The most common form of germicidal lamp looks similar to an ordinary fluorescent lamp but the tube contains no fluorescent phosphor. In addition, rather than being made of ordinary borosilicate glass, the tube is made of fused quartz or vycor 7913 glass. These two changes combine to allow the ...
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Cataracts
A cataract is a cloudy area in the lens of the eye that leads to a decrease in vision. Cataracts often develop slowly and can affect one or both eyes. Symptoms may include faded colors, blurry or double vision, halos around light, trouble with bright lights, and trouble seeing at night. This may result in trouble driving, reading, or recognizing faces. Poor vision caused by cataracts may also result in an increased risk of falling and depression. Cataracts cause 51% of all cases of blindness and 33% of visual impairment worldwide. Cataracts are most commonly due to aging but may also occur due to trauma or radiation exposure, be present from birth, or occur following eye surgery for other problems. Risk factors include diabetes, longstanding use of corticosteroid medication, smoking tobacco, prolonged exposure to sunlight, and alcohol. The underlying mechanism involves accumulation of clumps of protein or yellow-brown pigment in the lens that reduces transmission ...
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Laminar Chamber Simple
Laminar means "flat". Laminar may refer to: Terms in science and engineering: * Laminar electronics or organic electronics, a branch of material sciences dealing with electrically conductive polymers and small molecules * Laminar armour or "banded mail", armour made from horizontal overlapping rows or bands of solid armour plates * Laminar flame speed, a property of a combustible mixture * Laminar flow, a fluid flowing in parallel layers with no disruption between the layers * Laminar organization, the way certain tissues are arranged in layers * Laminar set family, a mathematical structure. *A common leaf shape. Proper nouns: * Laminar Research, a Columbia, South Carolina, software company * Icaro Laminar, an Italian hang glider design * Pazmany Laminar, a personal light aircraft designed by Ladislao Pazmany See also * Lamina (other) Lamina may refer to: Science and technology * Planar lamina, a two-dimensional planar closed surface with mass and density, in ma ...
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