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Kirby-Bauer Antibiotic Testing
The disk diffusion test (also known as the agar diffusion test, Kirby–Bauer test, disc-diffusion antibiotic susceptibility test, disc-diffusion antibiotic sensitivity test and KB test) is a culture-based microbiology assay used in diagnostic and drug discovery laboratories. In diagnostic labs, the assay is used to determine the susceptibility of bacteria isolated from a patient's infection to clinically approved antibiotics. This allows physicians to prescribe the most appropriate antibiotic treatment. In drug discovery labs, especially bioprospecting labs, the assay is used to screen biological material (e.g. plant extracts, bacterial fermentation broths) and drug candidates for antibacterial activity. When bioprospecting, the assay can be performed with paired strains of bacteria to achieve dereplication and provisionally identify antibacterial mechanism of action. In diagnostic laboratories, the test is performed by inoculating the surface of an agar plate with bac ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Kleihauer–Betke Test
The Kleihauer–Betke ("KB") test, Kleihauer–Betke ("KB") stain, Kleihauer test or acid elution test is a blood test used to measure the amount of fetal hemoglobin transferred from a fetus to a mother's bloodstream. It is usually performed on Rhesus blood group system, Rh-negative mothers to determine the required dose of Rho(D) immune globulin (RhIg) to inhibit formation of Rh antibody, antibodies in the mother and prevent Rh disease in future Rh-positive children. It is named after Enno Kleihauer and Klaus Betke who described it in 1957. Test details The KB test is the standard method of quantitating Fetal-maternal haemorrhage, fetal–maternal hemorrhage (FMH). It takes advantage of the differential resistance of fetal hemoglobin to acid. A standard blood smear is prepared from the mother's blood and exposed to an acid bath. This removes adult hemoglobin, but not fetal hemoglobin, from the red blood cells. Subsequent staining, using Shepard's method, makes fetal cells (conta ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Martinus Beijerinck
Martinus Willem Beijerinck (, 16 March 1851 – 1 January 1931) was a Dutch microbiologist and botanist who was one of the founders of virology and environmental microbiology. He is credited with the discovery of viruses, which he called "''contagium vivum fluidum''". Life Early life and education Born in Amsterdam, Beijerinck studied at the Technical School of Delft, where he was awarded the degree of Chemical Engineer in 1872. He obtained his Doctor of Science degree from the University of Leiden in 1877. At the time, Delft, then a Polytechnic, did not have the right to confer doctorates, so Leiden did this for them. He became a teacher in microbiology at the Agricultural School in Wageningen (now Wageningen University) and later at the ''Polytechnische Hogeschool Delft'' (Delft Polytechnic, currently Delft University of Technology) (from 1895). He established the Delft School of Microbiology. His studies of agricultural and industrial microbiology yielded fundamental disco ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Aseptic Technique
Asepsis is the state of being free from disease-causing micro-organisms (such as pathogenic bacteria, viruses, pathogenic fungi, and parasites). There are two categories of asepsis: medical and surgical. The modern day notion of asepsis is derived from the older antiseptic techniques, a shift initiated by different individuals in the 19th century who introduced practices such as the sterilizing of surgical tools and the wearing of surgical gloves during operations. The goal of asepsis is to eliminate infection, not to achieve sterility. Ideally, a surgical field is sterile, meaning it is free of all biological contaminants (e.g. fungi, bacteria, viruses), not just those that can cause disease, putrefaction, or fermentation. Even in an aseptic state, a condition of sterile inflammation may develop. The term often refers to those practices used to promote or induce asepsis in an operative field of surgery or medicine to prevent infection. History The modern concept of asepsis evol ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cell (biology)
The cell is the basic structural and functional unit of life forms. Every cell consists of a cytoplasm enclosed within a membrane, and contains many biomolecules such as proteins, DNA and RNA, as well as many small molecules of nutrients and metabolites.Cell Movements and the Shaping of the Vertebrate Body in Chapter 21 of Molecular Biology of the Cell '' fourth edition, edited by Bruce Alberts (2002) published by Garland Science. The Alberts text discusses how the "cellular building blocks" move to shape developing embryos. It is also common to describe small molecules such as ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
McFarland Standards
In microbiology, McFarland standards are used as a reference to adjust the turbidity of bacterial suspensions so that the number of bacteria will be within a given range to standardize microbial testing. An example of such testing is antibiotic susceptibility testing by measurement of minimum inhibitory concentration which is routinely used in medical microbiology and research. If a suspension used is too heavy or too dilute, an erroneous result (either falsely resistant or falsely susceptible) for any given antimicrobial agent could occur. Original McFarland standards were made by mixing specified amounts of barium chloride and sulfuric acid together. Mixing the two compounds forms a barium sulfate precipitate, which causes turbidity in the solution. A 0.5 McFarland standard is prepared by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl2•2H2O), with 9.95 mL of 1% sulfuric acid (H2SO4). Now there are McFarland standards prepared from suspensions of latex particles, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mueller–Hinton Agar
Mueller–Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing, specifically disk diffusion tests. It is also used to isolate and maintain ''Neisseria'' and ''Moraxella'' species. It typically contains: *2.0 g beef extract *17.5 g casein hydrolysate *1.5 g starch *17.0 g agar *1 liter of distilled water. *pH adjusted to neutral at 25 °C. Five percent sheep's blood and nicotinamide adenine dinucleotide may also be added when susceptibility testing is done on ''Streptococcus'' and '' Campylobacter'' species. It has a few properties that make it excellent for antibiotic use. First of all, it is a nonselective, nondifferential medium. This means that almost all organisms plated on it will grow. Additionally, it contains starch. Starch is known to absorb toxins released from bacteria, so that they cannot interfere with the antibiotics. Second, it is a loose agar. This allows for better diffusion of the antibiotics than mo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
KB Test
KB test can refer to two different medical tests: * The Kirby–Bauer test (agar diffusion test The disk diffusion test (also known as the agar diffusion test, Kirby–Bauer test, disc-diffusion antibiotic susceptibility test, disc-diffusion antibiotic sensitivity test and KB test) is a culture-based microbiology assay used in diagnos ...), a test of the antibiotic sensitivity of bacteria * The Kleihauer–Betke test ( acid elution test), a test of the amount of fetal hemoglobin transferred from a fetus to a mother's bloodstream {{disambig ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Minimum Inhibitory Concentration
In microbiology, the minimum inhibitory concentration (MIC) is the lowest concentration of a chemical, usually a drug, which prevents visible growth of a bacterium or bacteria. MIC depends on the microorganism, the affected human being (in vivo only), and the antibiotic itself. It is often expressed in micrograms per milliliter (μg/mL) or milligrams per liter (mg/L). The MIC is determined by preparing solutions of the chemical in vitro at increasing concentrations, incubating the solutions with separate batches of cultured bacteria, and measuring the results using agar dilution or broth microdilution. Results have been graded into susceptible (often called sensitive), increased exposure, or resistant to a particular antimicrobial by using a breakpoint. Breakpoints are agreed upon values, published in guidelines of a reference body, such as the U.S. Clinical and Laboratory Standards Institute (CLSI), the British Society for Antimicrobial Chemotherapy (BSAC) or the European Committee ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
British Society For Antimicrobial Chemotherapy
The British Society for Antimicrobial Chemotherapy (BSAC) is a UK-based multi-professional organisation with worldwide membership for clinicians and scientists with a specialist interest in antibiotic management and therapy. It is headquartered in Birmingham, UK. BSAC was founded in 1971 at a meeting in Prague. Activities Current BSAC activities include: * Publishes the ''Journal of Antimicrobial Chemotherapy''. * Organises numerous annual educational sessions, workshops and small conferences for clinicians, researchers, clinical scientists and academics. * Development of national standardised tests to determine micro-organism susceptibility to antibiotics, * Surveillance of resistance rates of organisms involved in the development of respiratory infections and bacteraemias. * Out-patient parenteral antibiotic therapy (OPAT) Initiative. The OPAT Initiative is involved in the development of a body of evidence that include good practice recommendations, a business case toolkit a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Deutsches Institut Für Normung
' (DIN; in English, the German Institute for Standardisation Registered Association) is the German national organization for standardization and is the German ISO member body. DIN is a German Registered Association ('' e.V.'') headquartered in Berlin. There are currently around thirty thousand DIN Standards, covering nearly every field of technology. History Founded in 1917 as the ' (NADI, "Standardisation Committee of German Industry"), the NADI was renamed ' (DNA, "German Standardisation Committee") in 1926 to reflect that the organization now dealt with standardization issues in many fields; viz., not just for industrial products. In 1975 it was renamed again to ', or 'DIN' and is recognised by the German government as the official national-standards body, representing German interests at the international and European levels. The acronym, 'DIN' is often incorrectly expanded as ' ("German Industry Standard"). This is largely due to the historic origin of the DIN as "NADI" ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Clinical And Laboratory Standards Institute
The Clinical and Laboratory Standards Institute (CLSI) is a volunteer-driven, membership-supported, not-for-profit, standards development organization. CLSI promotes the development and use of voluntary laboratory consensus standards and guidelines within the health care community. History In 1968, 31 clinicians and laboratory scientists representing 15 organizations met to develop a formal consensus process for standardization. In 1977, CLSI was accredited by the American National Standards Institute (ANSI) as a voluntary consensus standards organization. At about the same time, CLSI became the home of the National Reference System for the Clinical Laboratory (NRSCL), a collection of broadly understood reference systems intended to improve the comparability of test results, consistent with the needs of medical practice. CLSI is a global association of 1,500+ member organizations and individual members, as well as more than 2,000 volunteers. Until 2005, CLSI was called the Natio ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
World Health Organization
The World Health Organization (WHO) is a specialized agency of the United Nations responsible for international public health. The WHO Constitution states its main objective as "the attainment by all peoples of the highest possible level of health". Headquartered in Geneva, Switzerland, it has six regional offices and 150 field offices worldwide. The WHO was established on 7 April 1948. The first meeting of the World Health Assembly (WHA), the agency's governing body, took place on 24 July of that year. The WHO incorporated the assets, personnel, and duties of the League of Nations' Health Organization and the , including the International Classification of Diseases (ICD). Its work began in earnest in 1951 after a significant infusion of financial and technical resources. The WHO's mandate seeks and includes: working worldwide to promote health, keeping the world safe, and serve the vulnerable. It advocates that a billion more people should have: universal health care coverag ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
