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Kiyoshi Nagai
Kiyoshi Nagai (June 25, 1949 – September 27, 2019) was a Japanese structural biologist at the MRC Laboratory of Molecular Biology Cambridge, UK. He was known for his work on the mechanism of RNA splicing and structures of the spliceosome. Education Nagai studied at Osaka University and earned a Doctor of Philosophy under the supervision of Hideki Morimoto working on the allosteric effect in hemoglobin. Career and research In 1981 Nagai moved to the MRC Laboratory of Molecular Biology where he worked as a post-doc with Max Perutz on overproduction of eukaryotic proteins in ''E. coli.'' He produced recombinant hemoglobin and studied its properties and evolution by crystallography and mutagenesis. In 1987 he became a tenured group leader at the LMB and was joint head of the Division of Structural Studies from 2000 to 2010. He was appointed fellow of Darwin College, Cambridge in 1993. In 1990 his group solved the first structure of an RRM (RNA recognition motif) protein, U1 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Osaka University
, abbreviated as , is a public research university located in Osaka Prefecture, Japan. It is one of Japan's former Imperial Universities and a Designated National University listed as a "Top Type" university in the Top Global University Project. The university is often ranked among the top three public universities in Japan, along with the University of Tokyo and Kyoto University. It is ranked third overall among Japanese universities and 75th worldwide in the 2022 QS World University Rankings. Osaka University was one of the earliest modern universities in Japan at its founding in 1931. The history of the institution includes much older predecessors in Osaka such as the Kaitokudō founded in 1724 and the Tekijuku founded in 1838. In 2007, it merged with Osaka University of Foreign Studies and became the largest national university in Japan. Osaka University is one of the most productive research institutions in Japan. Numerous prominent scholars and scientists have attended or w ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Osaka
is a designated city in the Kansai region of Honshu in Japan. It is the capital of and most populous city in Osaka Prefecture, and the third most populous city in Japan, following Special wards of Tokyo and Yokohama. With a population of 2.7 million in the 2020 census, it is also the largest component of the Keihanshin Metropolitan Area, which is the second-largest metropolitan area in Japan and the 10th largest urban area in the world with more than 19 million inhabitants. Osaka was traditionally considered Japan's economic hub. By the Kofun period (300–538) it had developed into an important regional port, and in the 7th and 8th centuries, it served briefly as the imperial capital. Osaka continued to flourish during the Edo period (1603–1867) and became known as a center of Japanese culture. Following the Meiji Restoration, Osaka greatly expanded in size and underwent rapid industrialization. In 1889, Osaka was officially established as a municipality. The construc ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Darwin College, Cambridge
Darwin College is a constituent college of the University of Cambridge. Founded on 28 July 1964, Darwin was Cambridge University's first graduate-only college, and also the first to admit both men and women. The college is named after one of the university's most famous families and alumni, that of Charles Darwin. The Darwin family previously owned some of the land, Newnham Grange, on which the college now stands. The college has between 600 and 700 students, mostly studying for PhD or MPhil degrees with strengths in the sciences, humanities, and law. About half the students come from outside the United Kingdom, representing 80 nationalities as of 2016. Darwin is the largest graduate college of Cambridge. Darwin's sister college at Oxford University is Wolfson College. Members of Darwin College are termed ''Darwinians''. The college has several distinguished alumni including prominent heads of government and state, politicians, diplomats, and scientists from various countr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
European Molecular Biology Organisation
The European Molecular Biology Organization (EMBO) is a professional, non-profit organization of more than 1,800 life scientists. Its goal is to promote research in life science and enable international exchange between scientists. It co-funds courses, workshops and conferences, publishes five scientific journals and supports individual scientists. The organization was founded in 1964 and is a founding member of the Initiative for Science in Europe. the Director of EMBO is Fiona Watt, a stem cell researcher, professor at King's College London and a group leader at the European Molecular Biology Laboratory. Conferences and journals EMBO funds or co-funds over 90 meetings involving more than 11,000 participants every year. EMBO publishes five peer-reviewed scientific journals: ''The EMBO Journal'', ''EMBO Reports'', ''Molecular Systems Biology'', ''EMBO Molecular Medicine'', and ''Life Science Alliance'', History The European Molecular Biology Organization (EMBO) was launched i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Royal Society
The Royal Society, formally The Royal Society of London for Improving Natural Knowledge, is a learned society and the United Kingdom's national academy of sciences. The society fulfils a number of roles: promoting science and its benefits, recognising excellence in science, supporting outstanding science, providing scientific advice for policy, education and public engagement and fostering international and global co-operation. Founded on 28 November 1660, it was granted a royal charter by King Charles II as The Royal Society and is the oldest continuously existing scientific academy in the world. The society is governed by its Council, which is chaired by the Society's President, according to a set of statutes and standing orders. The members of Council and the President are elected from and by its Fellows, the basic members of the society, who are themselves elected by existing Fellows. , there are about 1,700 fellows, allowed to use the postnominal title FRS (Fellow of the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Shi Yigong
Shi Yigong (; born May 1967) is a Chinese biophysicist who serves as founding and the current president of Westlake University since April 2018. He previously served as vice president of Tsinghua University from 2015 to 2018 and dean of Tsinghua University School of Life Sciences from 2009 to 2016. Education Shi Yigong received a Bachelor of Science with majors in biology and mathematics from Tsinghua University in 1989 and a Doctor of Philosophy in molecular biophysics from Johns Hopkins University in 1995. During his graduate studies, he determined the crystal structure of several critical apoptotic proteins, including apaf-1, DIAP1, and the BIR3 domain of XIAP. Career Shi Yigong was the Warner-Lambert/Parke-Davis Professor in the department of Molecular Biology at Princeton University. In June 2008, he was selected as a Howard Hughes Medical Institute investigator. However, he rejected the award upon resigning his position at Princeton University in order to pursue ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Reinhard Lührmann
Reinhard is a German, Austrian, Danish, and to a lesser extent Norwegian surname (from Germanic ''ragin'', counsel, and ''hart'', strong), and a spelling variant of Reinhardt. Persons with the given name *Reinhard of Blankenburg (after 1107 – 1123), German bishop *Reinhard Böhler (1945–1995), German sidecarcross racer *Reinhard Bonnke (1940–2019), German evangelist * Rainhard Fendrich (born 1955), Austrian singer * Reinhard Gehlen (1902–1979), German spymaster * Reinhard Heydrich (1904–1942), German Nazi leader * Reinhard Mey (born 1942), German singer * Reinhard Mohn (1921–2009), German media tycoon *Reinhard Odendaal (born 1980), South African award-winning winemaker * Reinhard Scheer (1863–1928), German admiral *Reinhard Selten (1930–2016), German economist * Reinhard Strohm (born 1942), German musicologist *Reinhard Stupperich (born 1951), German classical archaeologist *Reinhard Wendemuth (born 1948), German rower Persons with the surname *Blaire R ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cryogenic Electron Microscopy
Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution. This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy for macromolecular structure determination without the need for crystallization. In 2017, the Nobel Prize in Chemistry was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution." ''Nature ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Prp8
Prp8 refers to both the Prp8 protein and Prp8 gene. Prp8's name originates from its involvement in pre-mRNA processing. The Prp8 protein is a large, highly conserved, and unique protein that resides in the catalytic core of the spliceosome and has been found to have a central role in molecular rearrangements that occur there. Prp8 protein is a major central component of the catalytic core in the spliceosome, and the spliceosome is responsible for splicing of precursor mRNA that contains introns and exons. Unexpressed introns are removed by the spliceosome complex in order to create a more concise mRNA transcript. Splicing is just one of many different post-transcriptional modifications that mRNA must undergo before translation. Prp8 has also been hypothesized to be a cofactor in RNA catalysis. History The systematic name for the PRP8 protein is YHR165C. Prp8 protein is coded by a single gene in humans with 42 exons. The size of Prp8 ranges between 230-280 kDa depending on the o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
U5 Spliceosomal RNA
U5 snRNA is a small nuclear RNA (snRNA) that participates in RNA splicing as a component of the spliceosome. It forms the U5 snRNP (''small nuclear ribonucleoprotein'') by associating with several proteins including Prp8 - the largest and most conserved protein in the spliceosome, Brr2 - a helicase Helicases are a class of enzymes thought to be vital to all organisms. Their main function is to unpack an organism's genetic material. Helicases are motor proteins that move directionally along a nucleic acid phosphodiester backbone, separatin ... required for spliceosome activation, Snu114, and the 7 Sm proteins. U5 snRNA forms a coaxially-stacked series of helices that project into the active site of the spliceosome. Loop 1, which caps this series of helices, forms 4-5 base pairs with the 5'-exon during the two chemical reactions of splicing. This interaction appears to be especially important during step two of splicing, exon ligation. References Further reading * * ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
U1 Spliceosomal RNA
U1 spliceosomal RNA is the small nuclear RNA (snRNA) component of U1 snRNP (''small nuclear ribonucleoprotein''), an RNA-protein complex that combines with other snRNPs, unmodified pre-mRNA, and various other proteins to assemble a spliceosome, a large RNA-protein molecular complex upon which splicing of pre-mRNA occurs. Splicing, or the removal of introns, is a major aspect of post-transcriptional modification, and takes place only in the nucleus of eukaryotes. Structure and function In humans, the U1 spliceosomal RNA is 164 bases long, forms four stem-loops, and possesses a 5'-trimethylguanosine five-prime cap. Bases 3 to 10 are a conserved sequence that base-pairs with the 5' splice site of introns during RNA splicing, and bases 126 to 133 form the Sm site, around which the Sm ring is assembled. Stem-loop I binds to the U1-70K protein, stem-loop II binds to the U1 A protein, stem-loops III and IV bind to the core RNP domain, a heteroheptameric Sm ring consisting of SmB/B', S ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
U2 Spliceosomal RNA
U2 spliceosomal snRNAs are a species of small nuclear RNA ( snRNA) molecules found in the major spliceosomal (Sm) machinery of virtually all eukaryotic organisms. ''In vivo'', U2 snRNA along with its associated polypeptides assemble to produce the U2 small nuclear ribonucleoprotein ( snRNP), an essential component of the major spliceosomal complex. The major spliceosomal-splicing pathway is occasionally referred to as U2 dependent, based on a class of Sm intron—found in mRNA primary transcripts—that are recognized exclusively by the U2 snRNP during early stages of spliceosomal assembly. In addition to U2 dependent intron recognition, U2 snRNA has been theorized to serve a catalytic role in the chemistry of pre-RNA splicing as well. Similar to ribosomal RNAs ( rRNAs), Sm snRNAs must mediate both RNA:RNA and RNA:protein contacts and hence have evolved specialized, highly conserved, primary and secondary structural elements to facilitate these types of interactions. Shortly after ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
