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Instruments Used In Microbiology
Instruments used specially in microbiology Microbiology () is the scientific study of microorganisms, those being unicellular (single cell), multicellular (cell colony), or acellular (lacking cells). Microbiology encompasses numerous sub-disciplines including virology, bacteriology, prot ... include:''Textbook of Microbiology'' by Ananthanarayan and Panikar, Instrument list As well as those "used in microbiological sterilization and disinfection" (see relevant section). Image gallery Image:Inoculation loop.JPG, An inoculation loop is used to transfer bacteria for microbiological culture. Image:Szalka petriego.jpg, Petri dish Image:Agarplates.jpg, Agar plate Image:Tuberculin Syringe.jpg, Tuberculin syringe Image:Trager & Jensen.jpg, Candle jar References {{MedInst Medical equipment Microbiology equipment ...
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Microbiology
Microbiology () is the scientific study of microorganisms, those being unicellular (single cell), multicellular (cell colony), or acellular (lacking cells). Microbiology encompasses numerous sub-disciplines including virology, bacteriology, protistology, mycology, immunology, and parasitology. Eukaryotic microorganisms possess membrane-bound organelles and include fungi and protists, whereas prokaryotic organisms—all of which are microorganisms—are conventionally classified as lacking membrane-bound organelles and include Bacteria and Archaea. Microbiologists traditionally relied on culture, staining, and microscopy. However, less than 1% of the microorganisms present in common environments can be cultured in isolation using current means. Microbiologists often rely on molecular biology tools such as DNA sequence based identification, for example the 16S rRNA gene sequence used for bacteria identification. Viruses have been variably classified as organisms, as they have ...
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Glass Slide
A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a microscope. Typically the object is mounted (secured) on the slide, and then both are inserted together in the microscope for viewing. This arrangement allows several slide-mounted objects to be quickly inserted and removed from the microscope, labeled, transported, and stored in appropriate slide cases or folders etc. Microscope slides are often used together with a cover slip or cover glass, a smaller and thinner sheet of glass that is placed over the specimen. Slides are held in place on the microscope's stage by slide clips, slide clamps or a cross-table which is used to achieve precise, remote movement of the slide upon the microscope's stage (such as in an automated/computer operated system, or where touching the slide with fingers is inappropriate either due to the risk of contamination or lack of precision ...
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Anaerobic
Anaerobic means "living, active, occurring, or existing in the absence of free oxygen", as opposed to aerobic which means "living, active, or occurring only in the presence of oxygen." Anaerobic may also refer to: * Anaerobic adhesive, a bonding agent that does not cure in the presence of air *Anaerobic respiration, respiration in the absence of oxygen, using some other molecule as the final electron acceptor **Anaerobic organism, any organism whose redox metabolism does not depend on free oxygen **Anammox, anaerobic ammonium oxidation, a globally important microbial process of the nitrogen cycle **Anaerobic filter, an anaerobic digester with a tank containing a filter medium where anaerobic microbes can establish themselves **Anaerobic digestion, the use of anaerobic bacteria to break down waste, with biogas as a byproduct ***Anaerobic clarigester, an anaerobic digester that treats dilute biodegradable feedstocks and allows different retention times for solids and liquids ***Anaer ...
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McIntosh And Filde's Anaerobic Jar
McIntosh and Fildes' anaerobic jar is an instrument used in the production of an anaerobic environment. This method of ''anaerobiosis'' as others is used to culture bacteria which die or fail to grow in presence of oxygen (''anaerobes''). It was originally introduced by James McIntosh, Paul Fildes and William Bulloch in 1916. McIntosh and Fildes, after whom the device has been named, published an improved version in 1921. Construction The jar, about 20″×12.5″ is made up of a metal. Its parts are as follows: #The body made up of metal (airtight) #The lid, also metal can be placed in an airtight fashion #A screw going through a curved metal strip to secure and hold the lid in place #A thermometer to measuring the internal temperature #A pressure gauge to measuring the internal pressure (or a side tube is attached to a manometer) # Another side tube for evacuation and introduction of gases (to a gas cylinder or a vacuum pump) #A wire cage hanging from the lid to hold a ...
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Colorimeter (chemistry)
A colorimeter is a device used in Colorimetry (chemical method), colorimetry that measures the absorbance of particular wavelengths of light by a specific Solution (chemistry), solution. It is commonly used to determine the concentration of a known solute in a given solution by the application of the Beer–Lambert law, which states that the concentration of a solute is proportional to the absorbance. Construction The essential parts of a colorimeter are: * a light source (often an ordinary low-voltage filament lamp); * an adjustable aperture; * a set of colored Filter (optics), filters; * a cuvette to hold the working solution; * a detector (usually a photoresistor) to measure the transmitted light; * a meter to display the output from the detector. In addition, there may be: * a voltage regulator, to protect the instrument from fluctuations in Mains power systems, mains voltage; * a second light path, cuvette and detector. This enables comparison between the working solut ...
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Lovibond Comparator
The Lovibond comparator is an example of a colorimeter made in Britain by The Tintometer Ltd. It was invented in the 19th century by Joseph Williams Lovibond anupdated versionsare still available. Description The device is used to determine the color of liquids. A sample is put in a glass tube. The tube is inserted in the comparator and compared with a series of coloured glass discs until the nearest possible match is found. Among other things, the device is used to determine the concentration of certain chemicals in solution. In this use, some assumptions are made about what is in the sample. Given those assumptions, the concentration will be indicated by the disc which best matches the color of the solution. There are a number of standard tests in which a sample to be tested is mixed a colour reagent. In such tests, the resulting color indicates the concentration of the sample under test. Results can be approximate compared to other testing techniques, but the comparator i ...
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Agglutination
In linguistics, agglutination is a morphological process in which words are formed by stringing together morphemes, each of which corresponds to a single syntactic feature. Languages that use agglutination widely are called agglutinative languages. Turkish is an example of an agglutinative language. The Turkish word ("from your houses") consists of the morphemes ''ev-ler-iniz-den,'' literally translated morpheme-by-morpheme as ''house-plural-your(plural)-from''. Agglutinative languages are often contrasted with isolating languages, in which words are monomorphemic, and fusional languages, in which words can be complex, but morphemes may correspond to multiple features. Examples of agglutinative languages Although agglutination is characteristic of certain language families, this does not mean that when several languages in a certain geographic area are all agglutinative they are necessarily related phylogenetically. In the past, this assumption led linguists to propose the ...
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Latex
Latex is an emulsion (stable dispersion) of polymer microparticles in water. Latexes are found in nature, but synthetic latexes are common as well. In nature, latex is found as a milky fluid found in 10% of all flowering plants (angiosperms). It is a complex emulsion that coagulates on exposure to air, consisting of proteins, alkaloids, starches, sugars, oils, tannins, resins, and gums. It is usually exuded after tissue injury. In most plants, latex is white, but some have yellow, orange, or scarlet latex. Since the 17th century, latex has been used as a term for the fluid substance in plants, deriving from the Latin word for "liquid". It serves mainly as defense against herbivorous insects. Latex is not to be confused with plant sap; it is a distinct substance, separately produced, and with different functions. The word latex is also used to refer to natural latex rubber, particularly non-vulcanized rubber. Such is the case in products like latex gloves, latex condoms ...
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Laminar Flow Cabinet
A laminar flow cabinet or tissue culture hood is a carefully enclosed bench designed to prevent contamination of semiconductor wafers, biological samples, or any particle sensitive materials. Air is drawn through a HEPA filter and blown in a very smooth, laminar flow towards the user. Due to the direction of air flow, the sample is protected from the user but the user is not protected from the sample. The cabinet is usually made of stainless steel with no gaps or joints where spores might collect. Such hoods exist in both horizontal and vertical configurations, and there are many different types of cabinets with a variety of airflow patterns and acceptable uses. Laminar flow cabinets may have a UV-C germicidal lamp to sterilize the interior and contents before usage to prevent contamination of the experiment. Germicidal lamps are usually kept on for fifteen minutes to sterilize the interior before the cabinet is used. The light must be switched off when the cabinet is being u ...
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Culture Media
A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss ''Physcomitrella patens''. Different types of media are used for growing different types of cells. The two major types of growth media are those used for cell culture, which use specific cell types derived from plants or animals, and those used for microbiological culture, which are used for growing microorganisms such as bacteria or fungi. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellular parasites and require a growth medium containing living cells. Types The most common growth media fo ...
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Inoculate
Inoculation is the act of implanting a pathogen or other microorganism. It may refer to methods of artificially inducing immunity against various infectious diseases, or it may be used to describe the spreading of disease, as in "self-inoculation," the spreading of disease from one part of the body to another, or even to the spreading of bacteria in a Petri dish for culturing purposes. The terms "inoculation", "vaccination", and "immunization" are often used synonymously, but there are some important differences among them. Inoculation is the act of implanting a disease inside a person or animal, vaccination is the act of implanting or giving someone a vaccine specifically, and immunization is what happens to the immune system as a result. Terminology Until the early 1800s inoculation referred only to variolation (from the Latin word ''variola'' = smallpox), the predecessor to the smallpox vaccine. The smallpox vaccine, introduced by Edward Jenner in 1796, was called cowpox inoc ...
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Inoculation Loop
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate.Ronald Westphal (1988): Microbiological Techniques in School', page 34. Document No. 28 in the series ''Science and Technology Education''. This process is called inoculation. The tool consists of a thin handle with a loop about 5 mm wide or smaller at the end. It was originally made of twisted metal wire (such as platinum, tungsten or nichrome), but disposable molded plastic versions are now common. See also * Cell spreader * Micropipette A pipette (sometimes spelled as pipett) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with diff ... References Laborat ...
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