Holographic Interference Microscopy
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Holographic Interference Microscopy
Holographic interference microscopy (HIM) is holographic interferometry applied for microscopy for visualization of phase micro-objects. Phase micro-objects are invisible because they do not change intensity of light, they insert only invisible phase shifts. The holographic interference microscopy distinguishes itself from other microscopy methods by using a hologram and the interference for converting invisible phase shifts into intensity changes. Other microscopy methods related to holographic interference microscopy are phase contrast microscopy and holographic interferometry. Holographic interference microscopy methods Holography was born as "new microscopy principle". D. Gabor invented holography for electron microscopy. For some reasons his idea is not applied in this branch of microscopy. But invention of holography opened up new possibilities in imaging of phase micro-objects due to the application of the holographic interference methods in microscopy that allow ...
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Holographic Interferometry
Holographic interferometry (HI)Powell RL & Stetson KA, 1965, J. Opt. Soc. Am., 55, 1593-8 is a technique which enables static and dynamic displacements of objects with optically rough surfaces to be measured to optical interferometric precision (i.e. to fractions of a wavelength of light). These measurements can be applied to stress, strain and vibration analysis, as well as to non-destructive testing and radiation dosimetry. It can also be used to detect optical path length variations in transparent media, which enables, for example, fluid flow to be visualised and analyzed. It can also be used to generate contours representing the form of the surface. Holography is the two-step process of recording a diffracted light field scattered from an object, and performing image rendering. This process can be achieved with traditional photographic plates or with a digital sensor array, in digital holography. If the recorded field is superimposed on the 'live field' scattered from the objec ...
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Contrast (vision)
Contrast is the contradiction in luminance or colour that makes an object (or its representation in an image or display) distinguishable. In visual perception of the real world, contrast is determined by the difference in the colour and brightness of the object and other objects within the same field of view. The human visual system is more sensitive to contrast than absolute luminance; we can perceive the world similarly regardless of the huge changes in illumination over the day or from place to place. The maximum ''contrast'' of an image is the contrast ratio or dynamic range. Images with a contrast ratio close to their medium's maximum possible contrast ratio experience a ''conservation of contrast'', wherein any increase in contrast in some parts of the image must necessarily result in a decrease in contrast elsewhere. Brightening an image will increase contrast in dark areas but decrease contrast in bright areas, while darkening the image will have the opposite effect. B ...
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Optical Microscope
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope. In high-power microscopes, both eyepieces typically show the same image, but with a stereo microscope, slightly different images are used to create a 3-D effect. A camera is typically used to capture the image (micrograph). The sample can be lit in a variety of ways. Transparent objects can be lit from below and solid objects can be lit with light coming through ( bright field) or around (dark field) the objective lens. Polarised ...
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Optical Aberrations
In optics, aberration is a property of optical systems, such as lenses, that causes light to be spread out over some region of space rather than focused to a point. Aberrations cause the image formed by a lens to be blurred or distorted, with the nature of the distortion depending on the type of aberration. Aberration can be defined as a departure of the performance of an optical system from the predictions of paraxial optics. In an imaging system, it occurs when light from one point of an object does not converge into (or does not diverge from) a single point after transmission through the system. Aberrations occur because the simple paraxial theory is not a completely accurate model of the effect of an optical system on light, rather than due to flaws in the optical elements. An image-forming optical system with aberration will produce an image which is not sharp. Makers of optical instruments need to correct optical systems to compensate for aberration. Aberration can be anal ...
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Homogeneous
Homogeneity and heterogeneity are concepts often used in the sciences and statistics relating to the uniformity of a substance or organism. A material or image that is homogeneous is uniform in composition or character (i.e. color, shape, size, weight, height, distribution, texture, language, income, disease, temperature, radioactivity, architectural design, etc.); one that is heterogeneous is distinctly nonuniform in at least one of these qualities. Heterogeneous Mixtures, in chemistry, is where certain elements are unwillingly combined and, when given the option, will separate. Etymology and spelling The words ''homogeneous'' and ''heterogeneous'' come from Medieval Latin ''homogeneus'' and ''heterogeneus'', from Ancient Greek ὁμογενής (''homogenēs'') and ἑτερογενής (''heterogenēs''), from ὁμός (''homos'', “same”) and ἕτερος (''heteros'', “other, another, different”) respectively, followed by γένος (''genos'', “kind”); - ...
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Refractive Index
In optics, the refractive index (or refraction index) of an optical medium is a dimensionless number that gives the indication of the light bending ability of that medium. The refractive index determines how much the path of light is bent, or refracted, when entering a material. This is described by Snell's law of refraction, , where ''θ''1 and ''θ''2 are the angle of incidence and angle of refraction, respectively, of a ray crossing the interface between two media with refractive indices ''n''1 and ''n''2. The refractive indices also determine the amount of light that is reflected when reaching the interface, as well as the critical angle for total internal reflection, their intensity ( Fresnel's equations) and Brewster's angle. The refractive index can be seen as the factor by which the speed and the wavelength of the radiation are reduced with respect to their vacuum values: the speed of light in a medium is , and similarly the wavelength in that medium is , where '' ...
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Optical Thickness
In physics, optical depth or optical thickness is the natural logarithm of the ratio of incident to ''transmitted'' radiant power through a material. Thus, the larger the optical depth, the smaller the amount of transmitted radiant power through the material. Spectral optical depth or spectral optical thickness is the natural logarithm of the ratio of incident to transmitted spectral radiant power through a material. Optical depth is dimensionless, and in particular is not a length, though it is a monotonically increasing function of optical path length, and approaches zero as the path length approaches zero. The use of the term "optical density" for optical depth is discouraged. In chemistry, a closely related quantity called "absorbance" or "decadic absorbance" is used instead of optical depth: the common logarithm of the ratio of incident to transmitted radiant power through a material, that is the optical depth divided by ln 10. Mathematical definitions Optical depth Op ...
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Interferogram Of Blood Erythrocyte
In physics, interference is a phenomenon in which two waves combine by adding their displacement together at every single point in space and time, to form a resultant wave of greater, lower, or the same amplitude. Constructive and destructive interference result from the interaction of waves that are correlated or coherent with each other, either because they come from the same source or because they have the same or nearly the same frequency. Interference effects can be observed with all types of waves, for example, light, radio, acoustic, surface water waves, gravity waves, or matter waves. Etymology The word ''interference'' is derived from the Latin words ''inter'' which means "between" and ''fere'' which means "hit or strike", and was coined by Thomas Young in 1801. Mechanisms The principle of superposition of waves states that when two or more propagating waves of the same type are incident on the same point, the resultant amplitude at that point is equal to ...
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Photographic Development
Photographic processing or photographic development is the chemical means by which photographic film or paper is treated after photographic exposure to produce a negative or positive image. Photographic processing transforms the latent image into a visible image, makes this permanent and renders it insensitive to light.Karlheinz Keller et al. "Photography" in Ullmann's Encyclopedia of Industrial Chemistry, 2005, Wiley-VCH, Weinheim. All processes based upon the gelatin silver process are similar, regardless of the film or paper's manufacturer. Exceptional variations include instant films such as those made by Polaroid and thermally developed films. Kodachrome required Kodak's proprietary K-14 process. Kodachrome film production ceased in 2009, and K-14 processing is no longer available as of December 30, 2010. Ilfochrome materials use the dye destruction process. Deliberately using the wrong process for a film is known as cross processing. Common processes All photographic ...
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Vibration
Vibration is a mechanical phenomenon whereby oscillations occur about an equilibrium point. The word comes from Latin ''vibrationem'' ("shaking, brandishing"). The oscillations may be periodic function, periodic, such as the motion of a pendulum—or random, such as the movement of a tire on a gravel road. Vibration can be desirable: for example, the motion of a tuning fork, the Reed (music), reed in a woodwind instrument or harmonica, a mobile phone, or the cone of a loudspeaker. In many cases, however, vibration is undesirable, wasting energy and creating unwanted sound. For example, the vibrational motions of engines, electric motors, or any Machine, mechanical device in operation are typically unwanted. Such vibrations could be caused by Engine balance, imbalances in the rotating parts, uneven friction, or the meshing of gear teeth. Careful designs usually minimize unwanted vibrations. The studies of sound and vibration are closely related. Sound, or pressure waves, are ge ...
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Intensity (physics)
In physics, the intensity or flux of radiant energy is the Power (physics), power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy. In the SI system, it has units watts per square metre (W/m2), or kilogram, kg⋅second, s−3 in SI base unit, base units. Intensity is used most frequently with waves such as acoustic waves (sound) or electromagnetic waves such as light or radio waves, in which case the time averaging, ''average'' power transfer over one Period (physics), period of the wave is used. ''Intensity'' can be applied to other circumstances where energy is transferred. For example, one could calculate the intensity of the kinetic energy carried by drops of water from a garden sprinkler. The word "intensity" as used here is not synonymous with "wikt:strength, strength", "wikt:amplitude, amplitude", "wikt:magnitude, magnitude", or "wikt:level, level", as it sometimes is in colloquial speech. Intensi ...
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Operational Definition
An operational definition specifies concrete, replicable procedures designed to represent a construct. In the words of American psychologist S.S. Stevens (1935), "An operation is the performance which we execute in order to make known a concept." For example, an operational definition of "fear" (the construct) often includes measurable physiologic responses that occur in response to a perceived threat. Thus, "fear" might be operationally defined as specified changes in heart rate, galvanic skin response, pupil dilation, and blood pressure. Overview An operational definition is designed to model or represent a concept or theoretical definition, also known as a construct. Scientists should describe the operations (procedures, actions, or processes) that define the concept with enough specificity such that other investigators can replicate their research. Operational definitions are also used to define system states in terms of a specific, publicly accessible process of preparation ...
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