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Fish DNA Barcoding
DNA barcoding methods for fish are used to identify groups of fish based on DNA sequences within selected regions of a genome. These methods can be used to study fish, as genetic material, in the form of environmental DNA (eDNA) or cells, is freely diffused in the water. This allows researchers to identify which species are present in a body of water by collecting a water sample, extracting DNA from the sample and isolating DNA sequences that are specific for the species of interest. Barcoding methods can also be used for biomonitoring and food safety validation, DNA barcoding in diet assessment, animal diet assessment, assessment of food webs and species distribution, and for detection of invasive species. In fish research, barcoding can be used as an alternative to traditional sampling methods. Barcoding methods can often provide information without damage to the studied animal. Aquatic environments have unique properties that affect how genetic material from organisms is distri ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
DNA Barcoding
DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise of DNA barcoding is that by comparison with a reference library of such DNA sections (also called "sequences"), an individual sequence can be used to uniquely identify an organism to species, just as a supermarket scanner uses the familiar black stripes of the UPC barcode to identify an item in its stock against its reference database. These "barcodes" are sometimes used in an effort to identify unknown species or parts of an organism, simply to catalog as many taxa as possible, or to compare with traditional taxonomy in an effort to determine species boundaries. Different gene regions are used to identify the different organismal groups using barcoding. The most commonly used barcode region for animals and some protists is a portion of the cytochrome ''c'' oxidase I (COI or COX1) gene, found in mitochondrial DNA. Other genes suitable for DNA barcoding ar ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spawn (biology)
Spawn is the Egg cell, eggs and Spermatozoa, sperm released or deposited into water by aquatic animals. As a verb, ''to spawn'' refers to the process of releasing the eggs and sperm, and the act of both sexes is called spawning. Most aquatic animals, except for aquatic mammals and marine reptile, reptiles, reproduce through the process of spawning. Spawn consists of the reproductive cells (gametes) of many aquatic animals, some of which will become fertilized and produce offspring. The process of spawning typically involves females releasing Ovum, ova (unfertilized eggs) into the water, often in large quantities, while males simultaneously or sequentially release spermatozoa (milt) to fertilize the eggs. Most fish reproduce by spawning, as do most other aquatic animals, including crustaceans such as crabs and shrimps, molluscs such as oysters and squid, echinoderms such as sea urchins and sea cucumbers, amphibians such as frogs and newts, aquatic insects such as mayflies and mos ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Algae DNA Barcoding
DNA barcoding of algae is commonly used for species identification and phylogenetic studies. Algae form a phylogenetically heterogeneous group, meaning that the application of a single universal barcode/ marker for species delimitation is unfeasible, thus different markers/barcodes are applied for this aim in different algal groups. Diatoms Diatom DNA barcoding is a method for taxonomical identification of diatoms even to species level. It is conducted using DNA or RNA followed by amplification and sequencing of specific, conserved regions in the diatom genome followed by taxonomic assignment. One of the main challenges of identifying diatoms is that it is often collected as a mixture of diatoms from several species. DNA metabarcoding is the process of identifying the individual species from a mixed sample of environmental DNA (also called eDNA) which is DNA extracted straight from the environment such as in soil or water samples. A newly applied method is diatom DNA m ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
GenBank
The GenBank sequence database is an open access, annotated collection of all publicly available nucleotide sequences and their protein translations. It is produced and maintained by the National Center for Biotechnology Information (NCBI; a part of the National Institutes of Health in the United States) as part of the International Nucleotide Sequence Database Collaboration (INSDC). GenBank and its collaborators receive sequences produced in laboratories throughout the world from more than 500,000 formally described species. The database started in 1982 by Walter Goad and Los Alamos National Laboratory. GenBank has become an important database for research in biological fields and has grown in recent years at an exponential rate by doubling roughly every 18 months. Release 250.0, published in June 2022, contained over 17 trillion nucleotide bases in more than 2,45 billion sequences. GenBank is built by direct submissions from individual laboratories, as well as from bulk submis ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Barcode Of Life Data System
The Barcode of Life Data System (commonly known as BOLD or BOLDSystems) is a web platform specifically devoted to DNA barcoding. It is a cloud-based data storage and analysis platform developed at the Centre for Biodiversity Genomics in Canada. It consists of four main modules, a data portal, an educational portal, a registry of BINs (putative species), and a data collection and analysis workbench which provides an online platform for analyzing DNA sequences. Since its launch in 2005, BOLD has been extended to provide a range of functionality including data organization, validation, visualization and publication. The most recent version of the system, version 4, launched in 2017, brings a set of improvements supporting data collection and analysis but also includes novel functionality improving data dissemination, citation, and annotation. Before November 16, 2020, BOLD already contained barcode sequences for 318,105 formally described species covering animals, plants, fungi, protist ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sequence Database
In the field of bioinformatics, a sequence database is a type of biological database that is composed of a large collection of computerized ("digital") nucleic acid sequences, protein sequences, or other polymer sequences stored on a computer. The UniProt database is an example of a protein sequence database. As of 2013 it contained over 40 million sequences and is growing at an exponential rate. Historically, sequences were published in paper form, but as the number of sequences grew, this storage method became unsustainable. Search Searching in a sequence database involves looking for similarities between a genomic/protein sequence and a query string and, finding the sequence in the database that "best" matches the target sequence (based on criteria which vary depending on the search method). The number of matches/hits is used to formulate a score that determines the similarity between the sequence query and the sequences in the sequence database. The main goal is to have a goo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
EDNA Metabarcoding
Metabarcoding is the barcoding of DNA/ RNA (or eDNA/ eRNA) in a manner that allows for the simultaneous identification of many taxa within the same sample. The main difference between barcoding and metabarcoding is that metabarcoding does not focus on one specific organism, but instead aims to determine species composition within a sample. A barcode consists of a short variable gene region (for example, see different markers/barcodes) which is useful for taxonomic assignment flanked by highly conserved gene regions which can be used for primer design. This idea of general barcoding originated in 2003 from researchers at the University of Guelph. The metabarcoding procedure, like general barcoding, proceeds in order through stages of DNA extraction, PCR amplification, sequencing and data analysis. Different genes are used depending if the aim is to barcode single species or metabarcoding several species. In the latter case, a more universal gene is used. Metabarcoding ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
EDNA Water Sampling (36024122736)
Edna or EDNA may refer to: Places United States *Edna, California, a census-designated place *Edna Lake, Idaho *Edna, Iowa, an unincorporated town in Lyon County * Edna Township, Cass County, Iowa *Edna, Kansas, a city *Edna, Kentucky, an unincorporated community *Edna Township, Otter Tail County, Minnesota * Edna Township, Barnes County, North Dakota *Edna, Texas, a city *Edna, Washington, an unincorporated community *Edna, West Virginia, an unincorporated community Outer space *445 Edna, an asteroid Arts and entertainment * ''Edna'' (album), a 2020 album by Headie One People and fictional characters *Edna (given name) Other uses * DNA#Extracellular nucleic acids – eDNA (extracellular DNA) *Edna High School, Edna, Texas *''Edna, the Inebriate Woman'', 1971 television drama * Electronic Declarations for National Authorities, a software developed by OPCW for national authorities *Environmental DNA (eDNA), DNA isolated from natural settings for the purpose of screening fo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
CITES
CITES (shorter name for the Convention on International Trade in Endangered Species of Wild Fauna and Flora, also known as the Washington Convention) is a multilateral treaty to protect endangered plants and animals from the threats of international trade. It was drafted as a result of a resolution adopted in 1963 at a meeting of members of the International Union for Conservation of Nature (IUCN). The convention was opened for signature in 1973 and CITES entered into force on 1 July 1975. Its aim is to ensure that international trade (import/export) in specimens of animals and plants included under CITES, does not threaten the survival of the species in the wild. This is achieved via a system of permits and certificates. CITES affords varying degrees of protection to more than 38,000 species. , Secretary-General of CITES is Ivonne Higuero. Background CITES is one of the largest and oldest conservation and sustainable use agreements in existence. There are three working langu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Shark Fins
Sharks are a group of elasmobranch fish characterized by a cartilaginous skeleton, five to seven gill slits on the sides of the head, and pectoral fins that are not fused to the head. Modern sharks are classified within the clade Selachimorpha (or Selachii) and are the sister group to the rays. However, the term "shark" has also been used to refer to all extinct members of Chondrichthyes with a shark-like morphology, such as hybodonts and xenacanths. The oldest modern sharks are known from the Early Jurassic. They range in size from the small dwarf lanternshark (''Etmopterus perryi''), a deep sea species that is only in length, to the whale shark (''Rhincodon typus''), the largest fish in the world, which reaches approximately in length. Sharks are found in all seas and are common to depths up to . They generally do not live in freshwater, although there are a few known exceptions, such as the bull shark and the river shark, which can be found in both seawater an ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ciguatera Fish Poisoning
Ciguatera fish poisoning (CFP), also known simply as ciguatera, is a foodborne illness caused by eating reef fish whose flesh is contaminated with certain toxins. Such individual fish are said to be ciguatoxic. Symptoms may include diarrhea, vomiting, numbness, itchiness, sensitivity to hot and cold, dizziness, and weakness. The onset of symptoms varies with the amount of toxin eaten from half an hour to up to two days. The diarrhea may last for up to four days. Some symptoms typically remain for a few weeks to months. Heart difficulties such as slow heart rate and low blood pressure may also occur. The specific toxins involved are ciguatoxin and maitotoxin. They are originally made by a small marine organism, ''Gambierdiscus toxicus'', that grows on and around coral reefs in tropical and subtropical waters. These are eaten by herbivorous fish which in turn are eaten by larger carnivorous fish. The toxins become more concentrated as they move up the food chain. The fish most oft ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fish As Food
Many species of fish are caught by humans and consumed as food in virtually all regions around the world. Fish has been an important dietary source of protein and other nutrients throughout human history. The English language does not have a special culinary name for food prepared from fish like with other animals (as with ''pig'' vs. ''pork''), or as in other languages (such as Spanish ''pez'' vs. '' pescado''). In culinary and fishery contexts, ''fish'' may include so-called shellfish such as molluscs, crustaceans, and echinoderms; more expansively, seafood covers both fish and other marine life used as food. Since 1961, the average annual increase in global apparent food fish consumption (3.2 percent) has outpaced population growth (1.6 percent) and exceeded consumption of meat from all terrestrial animals, combined (2.8 percent) and individually (bovine, ovine, porcine, etc.), except poultry (4.9 percent). In ''per capita'' terms, food fish consumption has grown from ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
