Forensic Limnology
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Forensic Limnology
Forensic limnology is a sub-field of freshwater ecology, which focuses especially on the presence of diatoms in crime scene samples and victims. Different methods are used to collect this data but all identify the ratios of different diatom colonies present in samples and match those samples with locations at the crime scene. Use of diatoms Diatoms are diverse microscopic algae with silica cell walls that have different characteristics such as color, shape, and size. There are 8,000 known species of diatoms. Diatoms do not have specialized nutrient and water conducting tissues, which affects their dispersion throughout ecosystems. These microscopic organisms mainly inhabit freshwater environments because of their inability to survive the cleaning agents present in domestic water sources. Benefits of diatoms Diatoms are identifiable based on each species' unique silica cell walls (known as "frustules") and vary depending on their environment. Because they have determinant properti ...
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Limnology
Limnology ( ; from Greek λίμνη, ''limne'', "lake" and λόγος, ''logos'', "knowledge") is the study of inland aquatic ecosystems. The study of limnology includes aspects of the biological, chemical, physical, and geological characteristics of fresh and saline, natural and man-made bodies of water. This includes the study of lakes, reservoirs, ponds, rivers, springs, streams, wetlands, and groundwater.Wetzel, R.G. 2001. Limnology: Lake and River Ecosystems, 3rd ed. Academic Press () Water systems are often categorized as either running (lotic) or standing (lentic). Limnology includes the study of the drainage basin, movement of water through the basin and biogeochemical changes that occur en route. A more recent sub-discipline of limnology, termed landscape limnology, studies, manages, and seeks to conserve these ecosystems using a landscape perspective, by explicitly examining connections between an aquatic ecosystem and its drainage basin. Recently, the need to underst ...
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Diatoms
A diatom (New Latin, Neo-Latin ''diatoma''), "a cutting through, a severance", from el, διάτομος, diátomos, "cut in half, divided equally" from el, διατέμνω, diatémno, "to cut in twain". is any member of a large group comprising several Genus, genera of algae, specifically microalgae, found in the oceans, waterways and soils of the world. Living diatoms make up a significant portion of the Earth's Biomass (ecology), biomass: they generate about 20 to 50 percent of the oxygen produced on the planet each year, take in over 6.7 billion metric tons of silicon each year from the waters in which they live, and constitute nearly half of the organic material found in the oceans. The Protist shell, shells of dead diatoms can reach as much as a half-mile (800 m) deep on the ocean floor, and the entire Amazon basin is fertilized annually by 27 million tons of diatom shell dust transported by transatlantic winds from the African Sahara, much of it from the Bodélé Dep ...
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Frustule
A frustule is the hard and porous cell wall or external layer of diatoms. The frustule is composed almost purely of silica, made from silicic acid, and is coated with a layer of organic substance, which was referred to in the early literature on diatoms as pectin, a fiber most commonly found in cell walls of plants. This layer is actually composed of several types of polysaccharides.Progress in Phycological Research: v. 7 (1991) by F.E. Round (Volume editor), David J. Chapman (Volume editor) The frustule's structure is usually composed of two overlapping sections known as thecae (or less formally as valves). The joint between the two thecae is supported by bands of silica (girdle bands) that hold them together. This overlapping allows for some internal expansion room and is essential during the reproduction process. The frustule also contains many pores called areolae and slits that provide the diatom access to the external environment for processes such as waste removal and muci ...
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Flora
Flora is all the plant life present in a particular region or time, generally the naturally occurring (indigenous) native plants. Sometimes bacteria and fungi are also referred to as flora, as in the terms '' gut flora'' or '' skin flora''. Etymology The word "flora" comes from the Latin name of Flora, the goddess of plants, flowers, and fertility in Roman mythology. The technical term "flora" is then derived from a metonymy of this goddess at the end of the sixteenth century. It was first used in poetry to denote the natural vegetation of an area, but soon also assumed the meaning of a work cataloguing such vegetation. Moreover, "Flora" was used to refer to the flowers of an artificial garden in the seventeenth century. The distinction between vegetation (the general appearance of a community) and flora (the taxonomic composition of a community) was first made by Jules Thurmann (1849). Prior to this, the two terms were used indiscriminately.Thurmann, J. (1849). ''Essai de ...
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Light Microscope
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope. In high-power microscopes, both eyepieces typically show the same image, but with a stereo microscope, slightly different images are used to create a 3-D effect. A camera is typically used to capture the image (micrograph). The sample can be lit in a variety of ways. Transparent objects can be lit from below and solid objects can be lit with light coming through ( bright field) or around (dark field) the objective lens. Polarised ...
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Post Mortem
An autopsy (post-mortem examination, obduction, necropsy, or autopsia cadaverum) is a surgical procedure that consists of a thorough examination of a corpse by dissection to determine the cause, mode, and manner of death or to evaluate any disease or injury that may be present for research or educational purposes. (The term "necropsy" is generally reserved for non-human animals). Autopsies are usually performed by a specialized medical doctor called a pathologist. In most cases, a medical examiner or coroner can determine the cause of death. However, only a small portion of deaths require an autopsy to be performed, under certain circumstances. Purposes of performance Autopsies are performed for either legal or medical purposes. Autopsies can be performed when any of the following information is desired: * Determine if death was natural or unnatural * Injury source and extent on the corpse * Manner of death must be determined * Post mortem interval * Determining the deceased's ...
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Phase Contrast Microscopy
__NOTOC__ Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than a vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium. Changes in amplitude (brightness) arise from the scattering and absorption of light, which is often wavelength-dependent and may give rise to colors. Photographic equipment and the human eye are only sensitive to amplitude variations. Without special arrangements, phase changes are therefore invisible. Yet, phase changes often convey important information. Phase-contrast microscopy is particularly important in biology. It reveals many cellular structures that are invisible with a bright-field microscope, as exemplif ...
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Ankistrodesmus
''Ankistrodesmus'' is a genus Genus ( plural genera ) is a taxonomic rank used in the biological classification of living and fossil organisms as well as viruses. In the hierarchy of biological classification, genus comes above species and below family. In binomial nom ... of green algae in the family Selenastraceae. Species * '' A. acerosus'' * '' A. acutissimus'' * '' A. amalloides'' * '' A. antarcticus'' * '' A. arcticus'' * '' A. bernardensis'' * '' A. bernardii'' * '' A. caribeum'' * '' A. chlorogonioides'' * '' A. cucumiformis'' * '' A. densus'' * '' A. dulcia'' * '' A. dybowskii'' * '' A. ecsediensis'' * '' A. extensus'' * '' A. falcatus'' * '' A. falciformia'' * '' A. fasciculatus'' * '' A. flexuosus'' * '' A. fractus'' * '' A. fusiformis'' * '' A. gracilis'' * '' A. hindakii'' * '' A. komarekii'' * '' A. lacuster'' * '' A. marinus'' * '' A. minutus'' * '' A. nannoselene'' * '' A. nivalis'' * '' A. pehrii'' * '' A. polymorphus'' * '' A. pseudosabulosum'' * '' ...
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Forensic Disciplines
Forensic science, also known as criminalistics, is the application of science to criminal and civil laws, mainly—on the criminal side—during criminal investigation, as governed by the legal standards of admissible evidence and criminal procedure. Forensic science is a broad field that includes; DNA analysis, fingerprint analysis, blood stain pattern analysis, firearms examination and ballistics, tool mark analysis, serology, toxicology, hair and fiber analysis, entomology, questioned documents, anthropology, odontology, pathology, epidemiology, footwear and tire tread analysis, drug chemistry, paint and glass analysis, digital audio video and photo analysis. Forensic scientists collect, preserve, and analyze scientific evidence during the course of an investigation. While some forensic scientists travel to the scene of the crime to collect the evidence themselves, others occupy a laboratory role, performing analysis on objects brought to them by other individuals. Still o ...
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