Eijkman Test
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Eijkman Test
Eijkman test, or differential coliform test, or confirmed Escherichia coli count, is a test used for the identification of coliform bacteria from warm-blooded animals based on the bacteria's ability to produce gas when grown in glucose media at 46°C (114.8°F). The test to determine whether coliform bacteria come from warm-blooded animals. By means of this test it can be readily established if water has been polluted by human and animal defecation containing coli bacilli. The test was introduced by Christiaan Eijkman Christiaan Eijkman ( , , ; 11 August 1858 – 5 November 1930) was a Dutch physician and professor of physiology whose demonstration that beriberi is caused by poor diet led to the discovery of antineuritic vitamins (thiamine). Together with S ... (1858–1930) in his paper in 1904. References {{Med-diagnostic-stub Medical tests ...
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Coliform Bacteria
Coliform bacteria are defined as either motile or non-motile Gram-negative non- spore forming Bacilli that possess β-galactosidase to produce acids and gases under their optimal growth temperature of 35-37°C. They can be aerobes or facultative aerobes, and are a commonly used indicator of low sanitary quality of foods, milk, and water. Coliforms can be found in the aquatic environment, in soil and on vegetation; they are universally present in large numbers in the feces of warm-blooded animals as they are known to inhabit the gastrointestinal system. While coliform bacteria are not normally causes of serious illness, they are easy to culture, and their presence is used to infer that other pathogenic organisms of fecal origin may be present in a sample, or that said sample is not safe to consume. Such pathogens include disease-causing bacteria, viruses, or protozoa and many multicellular parasites. Genera Typical genera include: * '' Citrobacter ''are peritrichous facultative ...
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Warm-blooded
Warm-blooded is an informal term referring to animal species which can maintain a body temperature higher than their environment. In particular, homeothermic species maintain a stable body temperature by regulating metabolic processes. The only known living homeotherms are birds and mammals, and the only living homeothermic lizard is the Argentine black and white tegu. Some extinct reptiles such as ichthyosaurs, pterosaurs, plesiosaurs and some non-avian dinosaurs are believed to have been homeotherms. Other species have various degrees of thermoregulation. Animal body temperature control varies by species, so the terms "warm-blooded" and " cold-blooded" (though still in everyday use) suggest a false idea of there being only two categories of body temperature control, and are no longer used scientifically. Terminology In general, warm-bloodedness refers to three separate categories of thermoregulation. *Endothermy is the ability of some creatures to control their body temp ...
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Water
Water (chemical formula ) is an inorganic, transparent, tasteless, odorless, and nearly colorless chemical substance, which is the main constituent of Earth's hydrosphere and the fluids of all known living organisms (in which it acts as a solvent). It is vital for all known forms of life, despite not providing food, energy or organic micronutrients. Its chemical formula, H2O, indicates that each of its molecules contains one oxygen and two hydrogen atoms, connected by covalent bonds. The hydrogen atoms are attached to the oxygen atom at an angle of 104.45°. "Water" is also the name of the liquid state of H2O at standard temperature and pressure. A number of natural states of water exist. It forms precipitation in the form of rain and aerosols in the form of fog. Clouds consist of suspended droplets of water and ice, its solid state. When finely divided, crystalline ice may precipitate in the form of snow. The gaseous state of water is steam or water vapor. Water co ...
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Defecation
Defecation (or defaecation) follows digestion, and is a necessary process by which organisms eliminate a solid, semisolid, or liquid waste material known as feces from the digestive tract via the anus. The act has a variety of names ranging from the common, like pooping or crapping, to the technical, e.g. bowel movement, to the obscene ('' shitting''), to the euphemistic ("dropping a deuce" or "taking a dump"). The topic, usually avoided among polite company, can become the basis for some potty humour. Humans expel feces with a frequency varying from a few times daily to a few times weekly. Waves of muscular contraction (known as ''peristalsis'') in the walls of the colon move fecal matter through the digestive tract towards the rectum. Undigested food may also be expelled this way, in a process called ''egestion''. When birds defecate, they also expel urine and urates in the same mass, whereas other animals may also urinate at the same time, but spatially separated. Defeca ...
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Coli Bacilli
''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus '' Escherichia'' that is commonly found in the lower intestine of warm-blooded organisms. Most ''E. coli'' strains are harmless, but some serotypes ( EPEC, ETEC etc.) can cause serious food poisoning in their hosts, and are occasionally responsible for food contamination incidents that prompt product recalls. Most strains do not cause disease in humans and are part of the normal microbiota of the gut; such strains are harmless or even beneficial to humans (although these strains tend to be less studied than the pathogenic ones). For example, some strains of ''E. coli'' benefit their hosts by producing vitamin K2 or by preventing the colonization of the intestine by pathogenic bacteria. These mutually beneficial relationships between ''E. coli ...
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Christiaan Eijkman
Christiaan Eijkman ( , , ; 11 August 1858 – 5 November 1930) was a Dutch physician and professor of physiology whose demonstration that beriberi is caused by poor diet led to the discovery of antineuritic vitamins (thiamine). Together with Sir Frederick Hopkins, he received the Nobel Prize for Physiology or Medicine in 1929 for the discovery of vitamins. Biography Early life and education Christiaan Eijkman was born on 11 August 1858, at Nijkerk, Netherlands as the seventh child of Christiaan Eijkman, the headmaster of a local school, and Johanna Alida Pool. His elder brother Johann Frederik Eijkman (1851–1915) was also a chemist. A year later, in 1859, the Eijkman family moved to Zaandam, where his father was appointed head of a newly founded school for advanced elementary education. It was here that Christiaan and his brothers received their early education. In 1875, after taking his preliminary examinations, Eijkman became a student at the Military Medical School of t ...
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