|
Clearing Factor
In centrifugation the clearing factor or k factor represents the relative pelleting efficiency of a given centrifuge rotor at maximum rotation speed. It can be used to estimate the time t (in hours) required for sedimentation of a fraction with a known sedimentation coefficient s (in svedbergs): : t = \frac The value of the clearing factor depends on the maximum angular velocity \omega of a centrifuge (in rad/s) and the minimum and maximum radius r of the rotor: : k = \frac \times \frac As the rotational speed of a centrifuge is usually specified in RPM, the following formula is often used for convenience: : k = \frac Centrifuge A centrifuge is a device that uses centrifugal force to separate various components of a fluid. This is achieved by spinning the fluid at high speed within a container, thereby separating fluids of different densities (e.g. cream from milk) or ... manufacturers usually specify the minimum, maximum and average radius of a rotor, as well as the k fa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Centrifugation
Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. The denser components of the mixture migrate away from the axis of the centrifuge, while the less dense components of the mixture migrate towards the axis. Chemists and biologists may increase the effective gravitational force of the test tube so that the precipitate (pellet) will travel quickly and fully to the bottom of the tube. The remaining liquid that lies above the precipitate is called a supernatant or supernate. There is a correlation between the size and density of a particle and the rate that the particle separates from a heterogeneous mixture, when the only force applied is that of gravity. The larger the size and the larger the density of the particles, the faster they separate from the mixture. By applying a larger effective gravitational force to the mixture, like a ce ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sedimentation
Sedimentation is the deposition of sediments. It takes place when particles in suspension settle out of the fluid in which they are entrained and come to rest against a barrier. This is due to their motion through the fluid in response to the forces acting on them: these forces can be due to gravity, centrifugal acceleration, or electromagnetism. Settling is the falling of suspended particles through the liquid, whereas sedimentation is the final result of the settling process. In geology, sedimentation is the deposition of sediments which results in the formation of sedimentary rock. The term is broadly applied to the entire range of processes that result in the formation of sedimentary rock, from initial erosion through sediment transport and settling to the lithification of the sediments. However, the strict geological definition of sedimentation is the mechanical deposition of sediment particles from an initial suspension in air or water. Sedimentation may pertain to ob ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sedimentation Coefficient
The sedimentation coefficient () of a particle characterizes its sedimentation during centrifugation. It is defined as the ratio of a particle's sedimentation velocity to the applied acceleration causing the sedimentation. : s = \frac The sedimentation speed is also the terminal velocity. It is constant because the force applied to a particle by gravity or by a centrifuge (typically in multiples of tens of thousands of gravities in an ultracentrifuge) is balanced by the viscous resistance (or "drag") of the fluid (normally water) through which the particle is moving. The applied acceleration can be either the gravitational acceleration , or more commonly the centrifugal acceleration . In the latter case, is the angular velocity of the rotor and is the distance of a particle to the rotor axis (radius). The viscous resistance for a spherical particle is given by Stokes' law: , where is the viscosity of the medium, is the radius of the particle and is the velocity of the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Svedberg
A Svedberg unit or svedberg (symbol S, sometimes Sv) is a non- SI metric unit for sedimentation coefficients. The Svedberg unit offers a measure of a particle's size indirectly based on its sedimentation rate under acceleration (i.e. how fast a particle of given size and shape settles to the bottom of a solution). The svedberg is a measure of time, defined as exactly 10−13 seconds (100 fs). For biological macromolecules and cell organelles like ribosomes, the sedimentation rate is typically measured as the rate of travel in a centrifuge tube subjected to high g-force. The svedberg (S) is distinct from the SI unit sievert or the non-SI unit sverdrup, which also use the symbol Sv. Naming The unit is named after the Swedish chemist Theodor Svedberg (1884–1971), winner of the 1926 Nobel Prize in chemistry for his work on disperse systems, colloids and his invention of the ultracentrifuge. Factors The Svedberg coefficient is a nonlinear function. A particle's mass, dens ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Angular Velocity
In physics, angular velocity or rotational velocity ( or ), also known as angular frequency vector,(UP1) is a pseudovector representation of how fast the angular position or orientation of an object changes with time (i.e. how quickly an object rotates or revolves relative to a point or axis). The magnitude of the pseudovector represents the ''angular speed'', the rate at which the object rotates or revolves, and its direction is normal to the instantaneous plane of rotation or angular displacement. The orientation of angular velocity is conventionally specified by the right-hand rule.(EM1) There are two types of angular velocity. * Orbital angular velocity refers to how fast a point object revolves about a fixed origin, i.e. the time rate of change of its angular position relative to the origin. * Spin angular velocity refers to how fast a rigid body rotates with respect to its center of rotation and is independent of the choice of origin, in contrast to orbital angular ve ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Radius
In classical geometry, a radius ( : radii) of a circle or sphere is any of the line segments from its center to its perimeter, and in more modern usage, it is also their length. The name comes from the latin ''radius'', meaning ray but also the spoke of a chariot wheel. as a function of axial position ../nowiki>" Spherical coordinates In a spherical coordinate system, the radius describes the distance of a point from a fixed origin. Its position if further defined by the polar angle measured between the radial direction and a fixed zenith direction, and the azimuth angle, the angle between the orthogonal projection of the radial direction on a reference plane that passes through the origin and is orthogonal to the zenith, and a fixed reference direction in that plane. See also *Bend radius *Filling radius in Riemannian geometry *Radius of convergence * Radius of convexity *Radius of curvature *Radius of gyration ''Radius of gyration'' or gyradius of a body about the axis of r ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Turbine
A turbine ( or ) (from the Greek , ''tyrbē'', or Latin ''turbo'', meaning vortex) is a rotary mechanical device that extracts energy from a fluid flow and converts it into useful work. The work produced by a turbine can be used for generating electrical power when combined with a generator.Munson, Bruce Roy, T. H. Okiishi, and Wade W. Huebsch. "Turbomachines." Fundamentals of Fluid Mechanics. 6th ed. Hoboken, NJ: J. Wiley & Sons, 2009. Print. A turbine is a turbomachine with at least one moving part called a rotor assembly, which is a shaft or drum with blades attached. Moving fluid acts on the blades so that they move and impart rotational energy to the rotor. Early turbine examples are windmills and waterwheels. Gas, steam, and water turbines have a casing around the blades that contains and controls the working fluid. Credit for invention of the steam turbine is given both to Anglo-Irish engineer Sir Charles Parsons (1854–1931) for invention of the reaction turbine, and to ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Revolutions Per Minute
Revolutions per minute (abbreviated rpm, RPM, rev/min, r/min, or with the notation min−1) is a unit of rotational speed or rotational frequency for rotating machines. Standards ISO 80000-3:2019 defines a unit of rotation as the dimensionless unit equal to 1, which it refers to as a revolution, but does not define the revolution as a unit. It defines a unit of rotational frequency equal to s−1. The superseded standard ISO 80000-3:2006 did however state with reference to the unit name 'one', symbol '1', that "The special name revolution, symbol r, for this unit is widely used in specifications on rotating machines." The International System of Units (SI) does not recognize rpm as a unit, and defines the unit of frequency, Hz, as equal to s−1. :\begin 1~&\text &&=& 60~&\text \\ \frac~&\text &&=& 1~&\text \end A corresponding but distinct quantity for describing rotation is angular velocity, for which the SI unit is the ra ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Centrifuge
A centrifuge is a device that uses centrifugal force to separate various components of a fluid. This is achieved by spinning the fluid at high speed within a container, thereby separating fluids of different densities (e.g. cream from milk) or liquids from solids. It works by causing denser substances and particles to move outward in the radial direction. At the same time, objects that are less dense are displaced and moved to the centre. In a laboratory centrifuge that uses sample tubes, the radial acceleration causes denser particles to settle to the bottom of the tube, while low-density substances rise to the top. A centrifuge can be a very effective filter that separates contaminants from the main body of fluid. Industrial scale centrifuges are commonly used in manufacturing and waste processing to sediment suspended solids, or to separate immiscible liquids. An example is the cream separator found in dairies. Very high speed centrifuges and ultracentrifuges able to provi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Laboratory Techniques
A laboratory (; ; colloquially lab) is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurement may be performed. Laboratory services are provided in a variety of settings: physicians' offices, clinics, hospitals, and regional and national referral centers. Overview The organisation and contents of laboratories are determined by the differing requirements of the specialists working within. A physics laboratory might contain a particle accelerator or vacuum chamber, while a metallurgy laboratory could have apparatus for casting or refining metals or for testing their strength. A chemist or biologist might use a wet laboratory, while a psychologist's laboratory might be a room with one-way mirrors and hidden cameras in which to observe behavior. In some laboratories, such as those commonly used by computer scientists, computers (sometimes supercomputers) are used for either simulations or the analysis of data. Scienti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
