Chemical Tests In Mushroom Identification
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Chemical Tests In Mushroom Identification
Chemical tests in mushroom identification are methods that aid in determining the variety of some fungi. The most useful tests are Melzer's reagent and potassium hydroxide. Ammonia Household ammonia can be used. A couple of drops are placed on the flesh. For example, '' Boletus spadiceus'' gives a fleeting blue to blue-green reaction. Iron salts Iron salts are used commonly in ''Russula'' and Bolete identification. It is best to dissolve the salts in water (typically a 10% solution) and then apply to the flesh, but it is sometimes possible to apply the dry salts directly to see a color change. For example, the white flesh of ''Boletus chrysenteron'' stains lemon-yellow or olive. Three results are expected with the iron salts tests: no change indicates a negative reaction; a color change to olive, green or blackish green; or a color change to reddish-pink. Meixner test for amatoxins The Meixner test (also known as the Wieland test) uses concentrated hydrochloric acid and ...
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Agaricus
''Agaricus'' is a genus of mushrooms containing both edible and poisonous species, with over 400 members worldwide and possibly again as many disputed or newly-discovered species. The genus includes the common ("button") mushroom (''Agaricus bisporus'') and the field mushroom ('' A. campestris''), the dominant cultivated mushrooms of the West. Members of ''Agaricus'' are characterized by having a fleshy cap or pileus, from the underside of which grow a number of radiating plates or gills, on which are produced the naked spores. They are distinguished from other members of their family, Agaricaceae, by their chocolate-brown spores. Members of ''Agaricus'' also have a stem or stipe, which elevates it above the object on which the mushroom grows, or substrate, and a partial veil, which protects the developing gills and later forms a ring or annulus on the stalk. The genus contains the most widely consumed and best-known mushroom today, '' A. bisporus'', with '' A. arvensis'', ...
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Melzer's Reagent
Melzer's reagent (also known as Melzer's iodine reagent, Melzer's solution or informally as Melzer's) is a chemical reagent used by mycologists to assist with the identification of fungi, and by phytopathologists for fungi that are plant pathogens. Composition Melzer's reagent is an aqueous solution of chloral hydrate, potassium iodide, and iodine. Depending on the formulation, it consists of approximately 2.50-3.75% potassium iodide and 0.75–1.25% iodine, with the remainder of the solution being 50% water and 50% chloral hydrate. Melzer's is toxic to humans if ingested due to the presence of iodine and chloral hydrate. Due to the legal status of chloral hydrate, Melzer's reagent is difficult to obtain in the United States. In response to difficulties obtaining chloral hydrate, scientists at Rutgers formulated Visikol (compatible with Lugol's iodine) as a replacement. In 2019, research showed that Visikol behaves differently to Melzer’s reagent in several key situations, notin ...
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Genus
Genus ( plural genera ) is a taxonomic rank used in the biological classification of extant taxon, living and fossil organisms as well as Virus classification#ICTV classification, viruses. In the hierarchy of biological classification, genus comes above species and below family (taxonomy), family. In binomial nomenclature, the genus name forms the first part of the binomial species name for each species within the genus. :E.g. ''Panthera leo'' (lion) and ''Panthera onca'' (jaguar) are two species within the genus ''Panthera''. ''Panthera'' is a genus within the family Felidae. The composition of a genus is determined by taxonomy (biology), taxonomists. The standards for genus classification are not strictly codified, so different authorities often produce different classifications for genera. There are some general practices used, however, including the idea that a newly defined genus should fulfill these three criteria to be descriptively useful: # monophyly – all descendants ...
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Section (biology)
In biology a section ( la, sectio) is a taxonomic rank that is applied differently in botany and zoology. In botany Within flora (plants), 'section' refers to a ''botanical'' rank below the genus, but above the species: * Domain > Kingdom > Division > Class > Order > Family > Tribe > Genus > Subgenus > Section > Subsection > Species In zoology Within fauna (animals), 'section' refers to a ''zoological'' rank below the order, but above the family: * Domain > Kingdom > Phylum > Class > Order > Section > Family > Tribe > Genus > Species In bacteriology The International Code of Nomenclature for Bacteria The International Code of Nomenclature of Prokaryotes (ICNP) formerly the International Code of Nomenclature of Bacteria (ICNB) or Bacteriological Code (BC) governs the scientific names for Bacteria and Archaea.P. H. A. Sneath, 2003. A short histor ... states that the Section rank is an informal one, between the subgenus and species (as in botany). References Botanical no ...
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Nitric Acid
Nitric acid is the inorganic compound with the formula . It is a highly corrosive mineral acid. The compound is colorless, but older samples tend to be yellow cast due to decomposition into oxides of nitrogen. Most commercially available nitric acid has a concentration of 68% in water. When the solution contains more than 86% , it is referred to as ''fuming nitric acid''. Depending on the amount of nitrogen dioxide present, fuming nitric acid is further characterized as red fuming nitric acid at concentrations above 86%, or white fuming nitric acid at concentrations above 95%. Nitric acid is the primary reagent used for nitration – the addition of a nitro group, typically to an organic molecule. While some resulting nitro compounds are shock- and thermally-sensitive explosives, a few are stable enough to be used in munitions and demolition, while others are still more stable and used as pigments in inks and dyes. Nitric acid is also commonly used as a strong oxidizing agen ...
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Aniline
Aniline is an organic compound with the formula C6 H5 NH2. Consisting of a phenyl group attached to an amino group, aniline is the simplest aromatic amine In organic chemistry, an aromatic amine is an organic compound consisting of an aromatic ring attached to an amine. It is a broad class of compounds that encompasses aniline Aniline is an organic compound with the formula C6 H5 NH2. Consi .... It is an industrially significant Commodity chemicals, commodity chemical, as well as a versatile starting material for fine chemical synthesis. Its main use is in the manufacture of precursors to polyurethane, dyes, and other industrial chemicals. Like most volatile amines, it has the odor of rotten fish. It Combustion, ignites readily, burning with a smoky flame characteristic of aromatic compounds. It is toxic to humans. Relative to benzene, it is electron-rich. It thus participates more rapidly in electrophilic aromatic substitution reactions. Likewise, it is also prone ...
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Julius Schäffer
Julius Schäffer (3 June 1882 – 21 October 1944) was a German mycologist. His contributions include studies on the Agaricales (gilled mushrooms), especially the genus ''Russula'', about which he wrote a monograph in 1933. Later, he revised the genus in the series ''Die Pilze Mitteleuropas'' (1926–1967); his notes were published posthumously by his wife Liesel in 1952 with the help of other mycologists. The work was considered the "authoritative treatment of the group for Central Europe". One of the ''Russula'' species that was first described in this publication was '' R. laeta''. Schäffer developed a chemical test to help with the identification of ''Agaricus'' species. A positive reaction of Schaeffer's test, which uses the reaction of aniline and nitric acid on the surface of the mushroom, is indicated by an orange to red color; it is characteristic of species in the section ''Flavescentes''. The compounds responsible for the reaction were named schaefferal A and B to honor Sc ...
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Boletes
{{refimprove, date=July 2020 A bolete is a type of mushroom, or fungal fruiting body. It can be identified thanks to a unique mushroom cap. The cap is clearly different from the stem. On the underside of the cap there is usually a spongy surface with pores, instead of the gills typical of mushrooms. However, there are some boletes that are gilled, such as species of ''Chroogomphus'', ''Gomphidius'', ''Paxillus'', ''Phylloporus'' and ''Hygrophoropsis aurantiaca''. "Bolete" is the English common name for fungus species whose mushroom caps have this appearance. The boletes are classified in the order Boletales. Not all members of the order Boletales are boletes. The micromorphology and molecular phylogeny of the order Boletales have established that it also contains many gilled, puffball, and other fruit body shapes. A similar pore surface is found in polypores, but these species generally have a different physical structure from boletes, and have different microscopic characte ...
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Cortinarius
''Cortinarius'' is a globally distributed genus of mushrooms in the family Cortinariaceae. It is suspected to be the largest genus of agarics, containing over 2,000 widespread species. A common feature among all species in the genus ''Cortinarius'' is that young specimens have a cortina (veil) between the cap and the stem, hence the name, meaning ''curtained''. Most of the fibres of the cortina are ephemeral and will leave no trace once gone, except for limited remnants on the stem or cap edge in some species. All have a rusty brown spore print. The common names cortinar and webcap refer to members of the genus. Due to dangerous toxicity of several species (such as ''Cortinarius orellanus'') and the fact that it is difficult to distinguish between various species of the genus, non-expert consumption of mushrooms from the genus is discouraged. Distinguishing features The veil protects the gills in younger specimens and usually disappears leaving little to no trace of itself as the ...
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Agaricus Subrutilescens
''Agaricus subrutilescens'', also known as the wine-colored agaricus, is a mushroom of the genus ''Agaricus''. It was first described scientifically in 1925 as ''Psalliota subrutilescens'', and later transferred to ''Agaricus'' in 1938. Description ''Agaricus subrutilescens'' has a cap that is across, dry, and has many wine to brown colored fibrils, especially near the center. The gills are close and white at first, turning pinkish and then dark brown in age. The stalk has a skirt-like ring and is long, thick, white, and covered with soft woolly scales below the ring. The flesh is white and does not stain, and the odor and taste are mild. The purplish fibrous cap and shaggy white stem differentiate this mushroom from others which resemble it. Similar species include ''Agaricus hondensis'' and ''Agaricus moelleri''. This mushroom is regarded variously described as edible, inedible, or responsible for causing gastric upset. Habitat and distribution The mushroom fruits in un ...
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Agaricus Xanthodermus
''Agaricus xanthodermus'', commonly known as the yellow-staining mushroom or simply the yellow-stainer, is a mushroom of the genus ''Agaricus'', which displays a strong yellow colouration at the base of the stem when cut. It is poisonous for most people, causing gastrointestinal upset, but can be eaten by some without apparent negative effect. Taxonomy This species was first officially defined under the name ''Agaricus xanthodermus'' in 1876 by Léon Gaston Genevier, in a letter published in the bulletin of the French Botanical Society. Genevier described the ''Agaricus'' mushrooms commonly eaten (perhaps sometimes inadvisedly) in the region of Nantes, and attempted to clarify the distinctions between them. He proposed a detailed reclassification into 5 species, including this new one. Apparently up until that time, these yellow-staining mushrooms were considered to be just varieties of other species which are edible: '' A. arvensis'', A. edulis', and '' A. silvi ...
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Phenol
Phenol (also called carbolic acid) is an aromatic organic compound with the molecular formula . It is a white crystalline solid that is volatile. The molecule consists of a phenyl group () bonded to a hydroxy group (). Mildly acidic, it requires careful handling because it can cause chemical burns. Phenol was first extracted from coal tar, but today is produced on a large scale (about 7 billion kg/year) from petroleum-derived feedstocks. It is an important industrial commodity as a precursor to many materials and useful compounds. It is primarily used to synthesize plastics and related materials. Phenol and its chemical derivatives are essential for production of polycarbonates, epoxies, Bakelite, nylon, detergents, herbicides such as phenoxy herbicides, and numerous pharmaceutical drugs. Properties Phenol is an organic compound appreciably soluble in water, with about 84.2 g dissolving in 1000 mL (0.895 M). Homogeneous mixtures of phenol and water at phenol ...
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